A framework for the identification of promising bio‐based chemicals

Wu, Wenzhao; Long, Matthew R.; Zhang, Xiaolin; Reed, Jennifer L.; Maravelias, Christos T.

doi:10.1002/bit.26779

Cited by 27 publications

(27 citation statements)

References 72 publications

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“…However, new technologies can be incorporated by changing model parameters and/or adding new constraints for the corresponding technologies. The insights from the base case results, as well as the predictions associated with the varying model parameters, provide important guidance on the selection of economically promising chemicals generated from microbial conversions [114], and on the design of cost efficient separation processes. Some insights regarding future research directions for technology enhancement as well as product titer improvements are also provided for low cost production of bio-based chemicals.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Yenkie

Maravelias

2019

BMC Chem Eng

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Recent advances in metabolic engineering have enabled the production of chemicals via bio-conversion using microbes. However, downstream separation accounts for 60-80% of the total production cost in many cases. Previous work on microbial production of extracellular chemicals has been mainly restricted to microbiology, biochemistry, metabolomics, or techno-economic analysis for specific product examples such as succinic acid, xanthan gum, lycopene, etc. In these studies, microbial production and separation technologies were selected apriori without considering any competing alternatives. However, technology selection in downstream separation and purification processes can have a major impact on the overall costs, product recovery, and purity. To this end, we apply a superstructure optimization based framework that enables the identification of critical technologies and their associated parameters in the synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions. We divide extracellular chemicals into three categories based on their physical properties, such as water solubility, physical state, relative density, volatility, etc. We analyze three major extracellular product categories (insoluble light, insoluble heavy and soluble) in detail and provide suggestions for additional product categories through extension of our analysis framework. The proposed analysis and results provide significant insights for technology selection and enable streamlined decision making when faced with any microbial product that is released extracellularly. The parameter variability analysis for the product as well as the associated technologies and comparison with novel alternatives is a key feature which forms the basis for designing better bioseparation strategies that have potential for commercial scalability and can compete with traditional chemical production methods.

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Section: Discussionmentioning

confidence: 99%

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Yenkie

Maravelias

2019

BMC Chem Eng

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“…Estimation of raw material costs for the two cases [7,9]. A sensitivity analysis of the influences on the production costs was performed for both the practical and ideal scenarios, and the resulting minimum and maximum production costs [13,20] that reflect the impact of common variations of the key input parameters from the base case are shown in Figures 5 and 6 The costs of materials and utilities as well as the yield of final products are included. Bio-fuel yield has the strongest positive influence on the production cost; this implies that slight improvements in the overall performance of bio-oil upgrading process could reduce the cost of fuel significantly.…”

Section: Operating Costsmentioning

confidence: 99%

Process Simulation and Economic Evaluation of Bio-Oil Two-Stage Hydrogenation Production

Pang

Zhang

et al. 2019

Applied Sciences

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Bio-oil hydrogenation upgrading process is a method that can convert crude bio-oil into high-quality bio-fuel oil, which includes two stages of mild and deep hydrogenation. However, coking in the hydrogenation process is the key issue which negatively affects the catalyst activity and consequently the degree of hydrogenation in both stages. In this paper, an Aspen Plus process simulation model was developed for the two-stage bio-oil hydrogenation demonstration plant which was used to evaluate the effect of catalyst coking on the bio-oil upgrading process and the economic performance of the process. The model was also used to investigate the effect of catalyst deactivation caused by coke deposition in the mild stage. Three reaction temperatures in the mild stage (250 °C, 280 °C, and 300 °C) were considered. The simulation results show that 45% yield of final product is obtained at the optimal reaction condition which is 280 °C for the mild stage and 400 °C for the deep stage. Economic analysis shows that the capital cost of industrial production is $15.2 million for a bio-oil upgrading plant at a scale of 107 thousand tons per year. The operating costs are predicted to be $1024.27 per ton of final product.

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“…A major hurdle to microbial production of biorenewable fuels and chemicals at economically viable titers, rates and yields is the fact that many of the target products, along with certain types of biomass-derived substrates, are harmful to the production organism [1][2][3][4]. One strategy for addressing this problem is to increase the robustness of the production organism to the problematic inhibitor, such as through evolutionary or targeted strain development, [3,[5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance

Santoscoy

Jarboe

2019

Journal of Industrial Microbiology and Biotechnology

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The economic viability of bio-production processes is often limited by damage to the microbial cell membrane and thus there is a demand for strategies to increase the robustness of the cell membrane. Damage to the microbial membrane is also a common mode of action by antibiotics. Membrane-impermeable DNA-binding dyes are often used to assess membrane integrity in conjunction with flow cytometry. We demonstrate that in situ assessment of the membrane permeability of E. coli to SYTOX Green is consistent with flow cytometry, with the benefit of lower experimental intensity, lower cost, and no need for a priori selection of sampling times. This method is demonstrated by the characterization of four membrane engineering strategies (deletion of aas, deletion of cfa, increased expression of cfa, and deletion of bhsA) for their effect on octanoic acid tolerance, with the finding that deletion of bhsA increased tolerance and substantially decreased membrane leakage. ABSTRACTThe economic viability of bio-production processes is often limited by damage to the microbial cell membrane and thus there is a demand for strategies to increase the robustness of the cell membrane. Damage to the microbial membrane is also a common mode of action by antibiotics. Membrane-impermeable DNA-binding dyes are often used to assess membrane integrity in conjunction with flow cytometry. We demonstrate that in situ assessment of the membrane permeability of E. coli to SYTOX Green is consistent with flow cytometry, with the benefit of lower experimental intensity, lower cost, and no need for a priori selection of sampling times. This method is demonstrated by the characterization of four membrane engineering strategies (deletion of aas, deletion of cfa, increased expression of cfa, and deletion of bhsA) for their effect on octanoic acid tolerance, with the finding that deletion of bhsA increased tolerance and substantially decreased membrane leakage.

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A framework for the identification of promising bio‐based chemicals

Cited by 27 publications

References 72 publications

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Synthesis and analysis of separation processes for extracellular chemicals generated from microbial conversions

Process Simulation and Economic Evaluation of Bio-Oil Two-Stage Hydrogenation Production

Streamlined assessment of membrane permeability and its application to membrane engineering of Escherichia coli for octanoic acid tolerance

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