A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats

Ahmed, Hijab; Hannan, Johanna L.; Apolzan, John W.; Osikoya, Oluwatobiloba; Cushen, Spencer C.; Romero, Steven A.; Goulopoulou, Styliani

doi:10.1152/ajpregu.00391.2018

Cited by 15 publications

(11 citation statements)

References 57 publications

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“…To determine the effects of pregnancy on adipose tissue morphology, we performed histological analysis as described previously (Ahmed et al . ). Briefly, samples of uterine PVAT and ovarian adipose tissue were embedded in paraffin, sectioned at 5 µm sections, and stained with haematoxylin and eosin by the Histology Core in the University of North Texas Health Science Centre.…”

Section: Methodsmentioning

confidence: 97%

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Uterine perivascular adipose tissue is a novel mediator of uterine artery blood flow and reactivity in rat pregnancy

Osikoya

Ahmed

Panahi

et al. 2019

The Journal of Physiology

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Key pointsr In vivo, uterine perivascular adipose tissue (PVAT) potentiates uterine artery blood flow in pregnant rats, although not in non-pregnant rats.r In isolated preparations, uterine PVAT has pro-contractile and anti-dilatory effects on uterine arteries.r Pregnancy induces changes in uterine arteries that makes them responsive to uterine PVAT signalling.Abstract An increase in uterine artery blood flow (UtBF) is a common and necessary feature of a healthy pregnancy. In the present study, we tested the hypothesis that adipose tissue surrounding uterine arteries (uterine perivascular adipose tissue; PVAT) is a novel local mediator of UtBF and uterine artery tone during pregnancy. In vivo experiments in anaesthetized Sprague-Dawley rats showed that pregnant animals (gestational day 16, term = 22--23 days) had a three-fold higher UtBF compared to non-pregnant animals. Surgical removal of uterine PVAT reduced UtBF only in pregnant rats. In a series of ex vivo bioassays, we demonstrated that uterine PVAT had pro-contractile and anti-dilatory effects on rat uterine arteries. In the presence of PVAT-conditioned media, isolated uterine arteries from both pregnant and non-pregnant rats had reduced vasodilatory responses. In non-pregnant rats, these responses were mediated at the level of uterine vascular smooth muscle, whereas, in pregnant rats, PVAT-media reduced endothelium-dependent relaxation. Pregnancy increased adipocyte size in ovarian adipose tissue but had no effect on uterine PVAT adipocyte morphology. In addition, pregnancy down-regulated the gene expression of metabolic adipokines in uterine but not in aortic PVAT. In conclusion, this is the first study to demonstrate that uterine PVAT plays a regulatory role in UtBF, at least in part, as a result of its actions on uterine artery tone. We propose that the interaction between the

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Section: Methodsmentioning

confidence: 97%

“…; Ahmed et al . ), adipocyte cell number and size (Resi et al . ), fat accretion, and lipid metabolism (Herrera, ).…”

Section: Introductionmentioning

confidence: 99%

Uterine perivascular adipose tissue is a novel mediator of uterine artery blood flow and reactivity in rat pregnancy

Osikoya

Ahmed

Panahi

et al. 2019

The Journal of Physiology

Self Cite

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“…We previously showed that HFD feeding from early age and continued through pregnancy resulted in a slight increase in fat weight and elevated blood pressure by the end of pregnancy at gestational day 19 in rats [ 5 ]. Others have also reported that HFD promotes increased fat weight in the P generation during their pregnancies [ 6 – 9 ]. However, it has not been examined whether HFD feeding during pregnancy in the P generation alters body or fat weights as well as blood pressure in the F1 offspring during their own pregnancies.…”

Section: Introductionmentioning

confidence: 99%

High-fat diet from parental generation exaggerates body and adipose tissue weights in pregnant offspring

Spradley

2020

PLoS ONE

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Parental high-fat diet (HFD) programs for obesity and hypertension in female offspring in rats, but it is unknown how the pregnancies of these offspring are impacted. Therefore, the hypothesis was tested that parental HFD exaggerates obesity and hypertension during pregnancy of the offspring. Wistar Hannover rat dams (the parental, P generation) were maintained on normal-fat diet (NFD) or HFD from weaning and were kept on respective diets through pregnancy and lactation. Their offspring (the first filial, F1 generation) were weaned onto the same diet as the P generation, or they were changed to the other diet to determine if combined HFD in the P and F1 generations exaggerates body weight and blood pressure levels during pregnancy in these offspring. This diet paradigm resulted in the following groups of pregnant F1 offspring: P-NFD/F1-NFD, P-HFD/F1-NFD, P-NFD/F1-HFD, and P-HFD/F1-HFD. Maternal body and adipose tissue weights were greatest in the P-HFD/F1-HFD group compared to the other 3 groups by the end of pregnancy. Plasma leptin and conscious mean arterial blood pressure were not significantly different between any group, although there was a main effect for increased blood pressure in the F1-HFD groups. Circulating levels of the antihypertensive pregnancy factor, placental growth factor (PlGF), were assessed. Although average PlGF levels were similar among all groups, correlative studies revealed that lower levels of PlGF were associated with higher blood pressure only in the P-HFD/F1-HFD group. In summary, HFD feeding from the P generation exaggerated HFD-induced body and adipose tissue weights in the pregnant offspring.

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“…We also added sucrose and hydrogenated fat. Several studies indicate that diets that are rich in sucrose or hydrogenated fat may induce endothelial dysfunction, raise blood pressure, and cause long-term cardiovascular and renal complications [32,33]. In addition to diet, one alternative to reduce rodent resistance to atherosclerosis is the co-administration of drugs that favor development of the disease.…”

Section: Discussionmentioning

confidence: 99%

Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats

et al. 2019

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Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats.

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A free-choice high-fat, high-sucrose diet induces hyperphagia, obesity, and cardiovascular dysfunction in female cycling and pregnant rats

Cited by 15 publications

References 57 publications

Uterine perivascular adipose tissue is a novel mediator of uterine artery blood flow and reactivity in rat pregnancy

Uterine perivascular adipose tissue is a novel mediator of uterine artery blood flow and reactivity in rat pregnancy

High-fat diet from parental generation exaggerates body and adipose tissue weights in pregnant offspring

Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats

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