Turbulence, quantified as the rate of dissipation of turbulent kinetic energy (e), was measured with 1400 temperature-gradient microstructure profiles obtained concurrently with time series measurements of temperature and current profiles, meteorology, and lake-atmosphere fluxes using eddy covariance in a 4 km 2 temperate lake during fall cooling. Ãw /kz, where u Ãw is the water friction velocity computed from wind shear stress, k is von Karman's constant, z is depth, and J B0 is surface buoyancy flux. Below a depth equal to |L MO | during cooling, dissipation was uniform with depth and controlled by buoyancy flux. Departures from similarity scaling enabled identification of additional processes that moderate near-surface turbulence including mixed layer deepening at the onset of cooling, high-frequency internal waves when the diurnal thermocline was adjacent to the air-water interface, and horizontal advection caused by differential cooling. The similarity scaling enables prediction of near-surface e as required for estimating the gas transfer coefficient using the surface renewal model and for understanding controls on scalar transport.