A fully automatable enzymatic method for DNA extraction from plant tissues

Abstract

Background: DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall around the plant cells. Currently used methods inevitably require a laborious mechanical grinding step, necessary to disrupt the cell wall for the release of DNA.

Comparative Effect of Ca, K, Mn and B on Post-Drought Stress Recovery in Tea [<i>Camellia sinensis</i> (L.) O <i>Kuntze</i>]

“…Since these phenolics can be oxidized to derivatives such as quinones, they have a higher potential to produce toxic oxygen species [53]. However, recently plants phenolic metabolites are also reported to function as free radical scavengers and mutagenesis inhibitors [54]. In case of tea, different catechins are the important phenolic compounds contributing to free radical protection and anti mutagenesis property of the plants.…”

Review: DNA Barcoding and Chromatography Fingerprints for the Authentication of Botanicals in Herbal Medicinal Products

“…A physical disruption method using liquid nitrogen is more preferred as it will help to prevent cross contamination more especially when multiple samples are used [242]. The use of chemical disruptors is also convenient as it can bypass the possibility of DNA shearing due to the mechanical force applied [243]. The prime limitation of using chemical disruptors is that they are expensive [240].…”

Effect ofCucumber mosaic virus(CMV) infection on antineoplastic alkaloids from periwinkle (Catharanthus roseusL.) cultured in the Mecca region and resistance induction by plant-growth-promoting rhizobacteria (PGPR)

“…Briefly, 2.5 mL of reverse-transcribed TNA mixtures were subjected to amplification with the addition of 2.5 mL of 10 £ Taq polymerase buffer (Promega Corporation, USA), with a final concentration of 1.5 mmol/L MgCl 2 for a total volume of 25 mL. PCR products were analysed with electrophoresis, stained with ethidium bromide and examined on an ultraviolet (UV) transilluminator [25].…”

“…The CMV inocula were prepared from CMV-infected top periwinkle leaves that yielded a positive reaction on ELISA. The periwinkle seedlings were conserved in a greenhouse at [24][25][26][27] C. ELISA was used in addition to symptom observation to check for the presence of the virus. Seedling pots were placed in a completely randomized block design with three seedlings per pot, three pots per replicate and three replicates per treatment, giving a total of 27 seedlings per treatment.…”