A Fully Automated Robotic System for High Throughput Fermentation

Zimmermann, Hartmut F.; Rieth, Jochen

doi:10.1016/j.cll.2006.12.007

Cited by 8 publications

(9 citation statements)

References 8 publications

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“…2001; Dieting et al. 2005; Zimmermann and Rieth 2006). However, techniques for growing filamentous fungi in microplate systems have lagged behind and have been limited to organisms which produce abundant, easily manipulated spores, e.g.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays

et al. 2008

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Aims: We asked to what extent does the application of the OSMAC (one strain, many compounds) approach lead to enhanced detection of antibiotics and secondary metabolites in fungi? Protocols for bacterial microfermentations were adapted to grow fungi in nutritional arrays. Methods and Results: Protocols for microfermentations of non‐sporulating fungi were validated using known antifungal‐producing fungi. Detection of antifungal activity was often medium dependent. The effects of medium arrays and numbers of strains on detection of antifungal signals were modelled by interpolation of rarefaction curves derived from matrices of positive and negative extracts. Increasing the number of fermentation media for any given strain increased the probability of detection of growth inhibition of Candida albicans. Increasing biodiversity increased detection of antifungal phenotypes, however, nutritional arrays could partly compensate for lost antibiotic phenotypes when biodiversity was limiting. Conclusions: Growth and extraction in microtiter plates can enable a discovery strategy emphasizing low‐cost medium arrays that can better exploit the metabolic potential of strains. Significance and Impact of the Study: Increasing fermentation parameters raise the probability of detecting bioactive metabolites from strains. The protocols can be used to pre‐select strains and their growth conditions for scale up that will most likely yield antibiotics and secondary metabolites.

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Section: Introductionmentioning

confidence: 99%

Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays

et al. 2008

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“…For instance, Puskeiler et al have developed such a system, consisting of a MBR device (called bioreactor block) integrated in a liquid-handling robot [ 20 ]. Zimmermann and Rieth established a system that utilizes microtiter plates and standard robotic equipment in a climate chamber to screen mutant libraries [ 21 ]. Another MBR device with 24 individual minireactors (M24, Applikon) can be combined with a plate crane and a single-channel pipettor for sampling and feeding [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

et al. 2009

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Background: In industry and academic research, there is an increasing demand for flexible automated microfermentation platforms with advanced sensing technology. However, up to now, conventional platforms cannot generate continuous data in high-throughput cultivations, in particular for monitoring biomass and fluorescent proteins. Furthermore, microfermentation platforms are needed that can easily combine cost-effective, disposable microbioreactors with downstream processing and analytical assays.

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“…In their realistic appraisal, Bolivar and co-workers suggest that, at the current stage of development, and where process development is concerned, namely at the key stage of screening enzymatic activity [ 144 , 145 ], the throughput available from microtiter plates is higher than that from microstructured reactors. The former configuration is furthermore more advanced when automation and integration with in-line analysis is considered, namely with commercially available systems [ 12 , 140 , 146 , 147 ]. Significant developments are taking place within the field of optical sensing systems, (such as microresonator sensing systems), with particular focus on the application to microfluidic devices, which are likely to result in the delivery of commercially available microfluidic devices with in-line analysis capability.…”

Section: Application Of Microstructured Devices To Bioprocesses: Smentioning

confidence: 99%

Microfluidic Devices: Useful Tools for Bioprocess Intensification

Marques

Fernandes

2011

Molecules

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The dawn of the new millennium saw a trend towards the dedicated use of microfluidic devices for process intensification in biotechnology. As the last decade went by, it became evident that this pattern was not a short-lived fad, since the deliverables related to this field of research have been consistently piling-up. The application of process intensification in biotechnology is therefore seemingly catching up with the trend already observed in the chemical engineering area, where the use of microfluidic devices has already been upgraded to production scale. The goal of the present work is therefore to provide an updated overview of the developments centered on the use of microfluidic devices for process intensification in biotechnology. Within such scope, particular focus will be given to different designs, configurations and modes of operation of microreactors, but reference to similar features regarding microfluidic devices in downstream processing will not be overlooked. Engineering considerations and fluid dynamics issues, namely related to the characterization of flow in microchannels, promotion of micromixing and predictive tools, will also be addressed, as well as reflection on the analytics required to take full advantage of the possibilities provided by microfluidic devices in process intensification. Strategies developed to ease the implementation of experimental set-ups anchored in the use of microfluidic devices will be briefly tackled. Finally, realistic considerations on the current advantages and limitation on the use of microfluidic devices for process intensification, as well as prospective near future developments in the field, will be presented.

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A Fully Automated Robotic System for High Throughput Fermentation

Cited by 8 publications

References 8 publications

Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays

Enhancement of antibiotic and secondary metabolite detection from filamentous fungi by growth on nutritional arrays

Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content

Microfluidic Devices: Useful Tools for Bioprocess Intensification

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