A fully integrated rapid on-chip antibiotic susceptibility test – A case study for Mycobacterium smegmatis

DNA nanotechnology for nucleic acid analysis: sensing of nucleic acids with DNA junction-probes

“…44 Several recent studies have demonstrated impressive LOD, ranging from 2.8 to 10 fM, and even reaching up to 10 CFU mL −1 . These achievements were realized by utilizing DNA linear probes with biotin-labeled reporters, 45 microfluidic chip devices, 46 or nanocomposites based on molybdenum disulfide, 47 with detection times spanning between 80 minutes to 4 hours. While these outcomes are noteworthy, there is room for improvement by employing stem-loop probes.…”

Simple and low‐cost antibiotic susceptibility testing for Mycobacterium tuberculosis using screen‐printed electrodes

“…In our previous study, we successfully performed AST assays for MSM after 1-day incubation, due to the much more rapid growth of this organism. 29,43 To specify the sensitivity of the proposed sensor, we examined EIS changes for different concentrations of TB (10 8 -10 3 ). The results demonstrate that the applied sensor platform detects TBs in the 10 8 -10 3 CFU/mL range with a significant trend (Figure 3a).…”

“…TB AST assays were, therefore, evaluated on the fourth day (96 h) post‐incubation. In our previous study, we successfully performed AST assays for MSM after 1‐day incubation, due to the much more rapid growth of this organism 29,43 …”

Microfluidic systems for infectious disease diagnostics

“…After thermal lysis, the samples were transferred into adjacent chambers where the resulting nucleic acid levels in response to the specific incubation conditions were measured via integrated Au microelectrodes. 232 A microfluidic PDMS chip for real-time monitoring of the growth dynamics and phenotypic alterations of M. smegmatis has been proposed as a tool for investigating drug-induced stress with single cell resolution. The device was composed of arrays of 0.9 μm high chambers for bacteria trapping and culture under different conditions, including drug exposure.…”