A Function for Ly-1+ B Cells

Ja, Hardin; K, Vos; Kawano, Yoshiaki; Dh, Sherr

doi:10.3181/00379727-195-43129c

Cited by 11 publications

(11 citation statements)

References 10 publications

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“…The failure of "anti-CD8 antibody adherent" cells to augment NPb PFC responses (13% NPb idiotype) and the recovery of this activity in the anti-CD8 nonadherent fraction demonstrated the specificity of this selection method. As in all previous studies (10,(25)(26)(27)(28)(29)(30), the magnitudes of the PFC responses were not significantly altered by idiotype-specific helper activity. These data support the hypothesis that a CD5' B cell population can provide NPb idiotype-specific activation signals to responding B cell subsets.…”

supporting

confidence: 67%

“…To approach this question, the maturational state of this B cell subset was defined by the ability of CD5' B cells to provide idiotype-specific activation signals to conventional, antigen-specific CD5-B cells (10,(25)(26)(27)(28)(29)(30). We reported previously that CD5+ B cell populations from normal or autoimmune mice or CD5+ hybridomas can provide activation signals to (4-hydroxy-3-nitrophenyl acetyl hapten (NP)-specific responder B cell populations that express members of the IgHhlinked idiotypic determinants on anti-NP antibod) (NP)b. CD5' B cell function is observed as an increase in the NP" idiotype portion of B cell responses to NP-Ficoll in the absence of T helper cells.…”

mentioning

confidence: 99%

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Interleukin 4 Induces the Maturation of Idiotype-Specific Regulatory B Cell Populations

Hardin

Gibson

Grant

et al. 1992

Experimental Biology and Medicine

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CD5+ B cells have been shown to be disproportionately associated with autoimmune diseases and transformation. In many cases, their apparent ability to bypass self-tolerance is manifested by the production of autoantibodies. These observations, plus the hypothesis that CD5+ B cells represent a distinct B cell lineage, encourage studies into the conditions and factors that influence their development. In the present study, we employed a well-established assay for murine CD5+ B cell function, i.e., their ability to augment the responses of IgHb-linked idiotypic determinants on anti-(4-hydroxy-3-nitrophenyl) acetyl (NP) antibody (Nbb) idiotype-bearing CD5- B cells to a T-independent antigen, together with multiple methods of cell surface phenotyping, to evaluate the potential for interleukin (IL) 4 to effect maturation of CD5+ B cells, CD5+, IgM+, Thy-1-, and NPb idiotype-specific cells panned on antibody-coated petri dishes or sorted by flow cytometry from spleen were capable of augmenting NPb idiotypic responses of NP-KLH-primed responder cells to NP-Ficoll. Splenic B cell populations depleted of CD5+ B cells failed to affect idiotype expression even after 2 days in culture, a time when a small percentage of CD5+ B cells appeared to be regenerated. However, idiotype-specific regulatory activity could be restored in CD5- splenic B cell populations by culture for 2 days with recombinant IL-4. Cells responsible for idiotype-specific regulatory activity after culture with IL-4 were in fact CD5+, IgM+, and Thy-1.2- B cells, demonstrating that IL-4 can drive the functional, if not the phenotypic, maturation of splenic B cells associated with the CD5+ B cell lineage. The results illustrate one possible mechanism by which T cells could control the maturation of cells belonging to the CD5+ B cell lineage.

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supporting

confidence: 67%

mentioning

confidence: 99%

Interleukin 4 Induces the Maturation of Idiotype-Specific Regulatory B Cell Populations

Hardin

Gibson

Grant

et al. 1992

Experimental Biology and Medicine

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“…Ly-1 + B cells appear to regulate the activity of Ly-1 -memory B cells [39], thus the differences observed in immune responses induced via the s.c. or i.p. routes may be a reflection of proportional differences in Ly-1 + and Ly-1-B cell populations in the respective inoculum sites.…”

Section: Secondary Immune Responses To Rabbit Ab2jil (Igg) Internal Imentioning

confidence: 99%

“…Ly-1 + B cells which can serve as regulators of B cell development and function make up 25-30% of the B cell population in the mouse peritoneal cavity [37] while Ly-1-B cells predominate in the spleen (98-99%) |38] and peripheral lymphoid tissues [39]. Ly-1 + B cells appear to regulate the activity of Ly-1 -memory B cells [39], thus the differences observed in immune responses induced via the s.c. or i.p.…”

Section: Secondary Immune Responses To Rabbit Ab2jil (Igg) Internal Imentioning

confidence: 99%

The Immune Response to Anti-Idiotype Antibodies Bearing an Internal Image Epitope of Tetanus Toxin/Toxoid

Poskitt¹,

Turnbull²,

Macdonald³

et al. 1991

Int Arch Allergy Immunol

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Primary immune responses to tetanus toxoid (TT) and primary and secondary immune responses to a rabbit TT internal image bearing anti-idiotype antibody (Ab2β1) inoculated in Freund’s complete adjuvant (FCA), saline (SAL) or syntex adjuvant formulation vehicle (SAF) via the intraperitoneal or subcutaneous route, were examined in mice. High anti-TT antibody (Ab3) titres are reported although the titre and persistence of the antibody response varied according to the adjuvant used in the priming and challenge inocula of Ab2β1. Mouse Ab3 antibodies were elicited in mice inoculated with rabbit Ab2β1 antibodies which in turn were elicited by an inoculum of mouse monoclonal anti-TT Ab1 antibody. Ab3 was shown to be identical to Ab1 by immunoblot analysis. Primary and secondary immune responses elicited by rabbit Ab2β1 antibody protected mice against a lethal dose of tetanus toxin.

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“…the roles played by many cell surface markers remain obscure. It is also suggested that these cells are functionally important to provide idiotype-specific activation signals to antigen-specific CD5-B cells (9). This antigen was initially described as a pan T cell marker.…”

mentioning

confidence: 99%

Zinc Depletion Modifies CD5 Expression by 70Z/3 Murine Pre-B Leukemia Cell Line

Jyonouchi

Voss

Good

1991

Experimental Biology and Medicine

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CD5 expression on B cells is regulated by certain humoral factors. In a pre-B leukemia cell line 70Z/3, we found that interleukin 4 down-regulates it. Herein, we report that zinc influences spontaneous CD5 expression by this cell line as well as actions of these factors on CD5 expression considerably. In zinc-depleted culture media, spontaneous CD5 expression by 70Z/3 cells was enhanced. In contrast, the down-regulatory action of interleukin 4 was significantly reduced under culture conditions of zinc depletion. The supplementation of zinc to physiologic concentrations (1 to 2 microM) abolished such effects of zinc-depleted medium. The reduction of the suppressive action of interleukin 4 was observed at the level of gene expression. However, CD5 mRNA expression enhanced by lipopolysaccharide or NZB-SF was not further enhanced under conditions of zinc deficiency. These observations may suggest that CD5 expression by malignant or even normal B cells may be influenced by cellular/serum zinc levels.

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A Function for Ly-1+ B Cells

Cited by 11 publications

References 10 publications

Interleukin 4 Induces the Maturation of Idiotype-Specific Regulatory B Cell Populations

Interleukin 4 Induces the Maturation of Idiotype-Specific Regulatory B Cell Populations

The Immune Response to Anti-Idiotype Antibodies Bearing an Internal Image Epitope of Tetanus Toxin/Toxoid

Zinc Depletion Modifies CD5 Expression by 70Z/3 Murine Pre-B Leukemia Cell Line

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