A functional analysis of angiotensin II targets through genome wide surveys

Sterio, Jelena; Wang, Chenguang; Francis, Robert C.; Murray, B. Stuart; Batlle, Daniel; Mayhew, Bobbie; Bassett, Mary H.; Rainey, William E.; Pestell, Richard G.

doi:10.1016/s0895-7061(01)01946-x

Cited by 1 publication

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“…In most cases this requires a means of selecting equally among structures irrespective of their size. The stereology probe used to make this selection is the disector (Sterio, 1984) or more typically the optical disector (Gundersen, 1986; Dorph-Petersen et al, 2001), consisting of an unbiased counting frame located within an optical plane of a thick section and at least one subsequent optical plane in the section, shown in Figures 4E,F. In each plane, any structure falling within the counting frame, or in contact with either of the two green inclusion sides, but not the two red exclusion lines and their extensions, and not in contact with the subsequent plane, is counted.…”

Section: Multiple Label Fluorescence Confocal Microscopy For Quantifimentioning

confidence: 99%

Mapping synaptic pathology within cerebral cortical circuits in subjects with schizophrenia

Sweet

Fish

Lewis

2010

Front. Hum. Neurosci.

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Converging lines of evidence indicate that schizophrenia is characterized by impairments of synaptic machinery within cerebral cortical circuits. Efforts to localize these alterations in brain tissue from subjects with schizophrenia have frequently been limited to the quantification of structures that are non-selectively identified (e.g., dendritic spines labeled in Golgi preparations, axon boutons labeled with synaptophysin), or to quantification of proteins using methods unable to resolve relevant cellular compartments. Multiple label fluorescence confocal microscopy represents a means to circumvent many of these limitations, by concurrently extracting information regarding the number, morphology, and relative protein content of synaptic structures. An important adaptation required for studies of human disease is coupling this approach to stereologic methods for systematic random sampling of relevant brain regions. In this review article we consider the application of multiple label fluorescence confocal microscopy to the mapping of synaptic alterations in subjects with schizophrenia and describe the application of a novel, readily automated, iterative intensity/morphological segmentation algorithm for the extraction of information regarding synaptic structure number, size, and relative protein level from tissue sections obtained using unbiased stereological principles of sampling. In this context, we provide examples of the examination of pre- and post-synaptic structures within excitatory and inhibitory circuits of the cerebral cortex.

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Section: Multiple Label Fluorescence Confocal Microscopy For Quantifimentioning

confidence: 99%