A functional homologue of Arabidopsis TTG1 from Freesia interacts with bHLH proteins to regulate anthocyanin and proanthocyanidin biosynthesis in both Freesia hybrida and Arabidopsis thaliana

Shan, Xiaotong; Li, Yueqing; Song, Yang; Gao, Ruifang; Zhou, Liudi; Bao, Tingting; Han, Tian; Wang, Shucai; Gao, Xiang; Wang, Li

doi:10.1016/j.plaphy.2019.05.015

Cited by 51 publications

(48 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The co-transfection of Gal4: GUS and GD-fused FhTT8L , FhTTG1 , FhMYB27ΔC or FhMYBx showed no significant GUS expression (Fig. 3c ) 39 , 49 , 50 . However, high GUS activity was detected when GD-tagged FhTT8L and the reformed transactivator FhMYB27 Δ C-VP16 or FhMYBx-VP16 were cotransfected together as effectors, indicating FhTT8L might be the bridge linking the MYB repressors to MBW complex (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…2a ). The constructed heat map allowed visualization of the general gene expression tendencies of FhMYB27 and FhMYBx , as well as Freesia anthocyanin biosynthetic genes and the earlier characterized MBW components 39 , 48 , 50 . The FhMYB27 and FhMYBx were grouped together with LBGs and MBW components, which were shown to concurrently increase during flower development (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the versatile transient expression system based on the flower petals or protoplasts isolated from Freesia callus partially overcomes the obstacle of the recalcitrant characters of monocots against Agrobacterium and immensely accelerates the molecular investigations in Freesia per se 47 , 48 . To date, the components of MBW activator complex have been elucidated in Freesia and the regulatory characteristics are conserved but with slight differences compared with their orthologs in model plants 39 , 48 – 50 . However, the hierarchical and feedback gene regulatory network of Freesia anthocyanin biosynthesis including MYB repressors remains unresolved.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida

Shan

Gao

et al. 2020

Commun Biol

Self Cite

View full text Add to dashboard Cite

Floral anthocyanin has multiple ecological and economic values, its biosynthesis largely depends on the conserved MYB-bHLH-WD40 (MBW) activation complex and MYB repressors hierarchically with the MBW complex. In contrast to eudicots, the MBW regulatory network model has not been addressed in monocots because of the lack of a suitable system, as grass plants exhibit monotonous floral pigmentation patterns. Presently, the MBW regulatory network was investigated in a non-grass monocot plant, Freesia hybrida . FhMYB27 and FhMYBx with different functional manners were confirmed to be anthocyanin related R2R3 and R3 MYB repressors, respectively. Particularly, FhMYBx could obstruct the formation of positive MBW complex by titrating bHLH proteins, whereas FhMYB27 mainly defected the activator complex into suppressor via its repression domains in C-terminus. Furthermore, the hierarchical and feedback regulatory loop was verified, indicating the synergistic and sophisticated regulatory network underlying Freesia anthocyanin biosynthesis was quite similar to that reported in eudicot plants.

show abstract

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida

Shan

Gao

et al. 2020

Commun Biol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Anthocyanins are the major class of water-soluble pigments found in plants, and belong to the flavonoid polyphenols [7]. The metabolic pathway of anthocyanin is relatively well documented in model plants [8,9]. The structural genes for anthocyanin biosynthesis include chalcone synthase (CHS), chalcone isomerase (CHI), flavonoid-3-hydroxylase (F3H), flavonoid-3′-hydroxylase (F3'H), flavonoid-3′,5′-hydroxylase (F3'5'H), and dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS) [10].…”

Section: Introductionmentioning

confidence: 99%

Functional MYB transcription factor gene HtMYB2 is associated with anthocyanin biosynthesis in Helianthus tuberosus L

et al. 2020

View full text Add to dashboard Cite

Background: Tuber color is an important trait for Helianthus tuberosus L. (Jerusalem artichoke). Usually, purple tubers with high anthocyanin content are more nutritious than white tuber. But, the molecular mechanism underlying it is unknown. Results: In the current study, high-throughput RNA-sequencing was used to compare the transcriptomes between plants with tubers with red or white epidermis. Compared with the white-skinned tubers of cultivar QY3, anthocyanin biosynthesis structural genes had greater expression in the red-skinned tubers of cultivar QY1, indicating that the anthocyanin biosynthesis pathway was activated in 'QY1'; quantitative PCR confirmed this difference in expression. HtMYB2 (Unigene44371_All) was the only MYB transcription factor, homologous to the MYB transcription factor regulating anthocyanin biosynthesis, expressed in the red tuber epidermis of 'QY1'. The anthocyanin concentration in the root, stem, leaf, flower, and tuber epidermis of 'QY1' was higher than in 'QY3', especially tuber epidermis. Correspondingly, HtMYB2 had greater expression in these tissues of 'QY1' than in 'QY3'. The expression of HtMYB2 was associated with anthocyanin accumulation in the different tissues. Overexpression of HtMYB2 activated the anthocyanin biosynthesis pathway, accumulating the pigment in leaves of transgenic tobacco, supporting the model that HtMYB2 regulated anthocyanin biosynthesis. Further experiments found that HtMYB2 had the same coding sequence and genomic sequence in 'QY1' and 'QY3', but that there were several single nucleotide polymorphisms and one insertiondeletion (indel) mutation of 21 nucleotides in the promoter region between the two alleles. The deletion of three nucleotides "AAA" made the promoter of 'QY1' predicted to contain one more possible promoter region. A specific primer, based on the indel, could differentiate between cultivars with red or white tuber epidermis. The genetic variation in HtMYB2 was associated with the tuber skin color in a natural population. Conclusions: RNA-seq can successfully isolate the candidate gene (HTMYB2) controlling anthocyanin biosynthesis in purple epidermis of Jerusalem artichoke tuber. HTMYB2 can regulate anthocyanin biosynthesis in plants and is closely related to the formation of purple phenotype in tubers. This study should be useful in understanding the genetic mechanism underlying different tuber skin colors and in breeding new H. tuberosus cultivars with different tuber skin colors.

show abstract

“…Anthocyanins are the major class of water-soluble pigments found in plants, and belong to the avonoid polyphenols [7]. The metabolic pathway of anthocyanin is relatively well documented in model plants [8,9]. The structural genes for anthocyanin biosynthesis include chalcone synthase (CHS), chalcone isomerase (CHI), avonoid-3-hydroxylase (F3H), avonoid-3'-hydroxylase (F3'H), avonoid-3',5'hydroxylase (F3'5'H), and dihydro avonol 4-reductase (DFR), anthocyanidin synthase (ANS) [10].…”

Section: Introductionmentioning

confidence: 99%

Article Functional MYB transcription factor gene HtMYB2 is associated with anthocyanin biosynthesis in Helianthus tuberosus L.

Gao

Sun

Zong³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Tuber color is an important trait for Helianthus tuberosus L. (Jerusalem artichoke). Usually, purple tubers with high anthocyanin content are more nutritious than white tuber. But, the molecular mechanism underlying it is unknown. Results: In the current study, high-throughput RNA-sequencing was used to compare the transcriptomes between plants with tubers with red or white epidermis. Compared with the white-skinned tubers of cultivar QY3, anthocyanin biosynthesis structural genes had greater expression in the red-skinned tubers of cultivar QY1, indicating that the anthocyanin biosynthesis pathway was activated in ‘QY1’; quantitative PCR confirmed this difference in expression. HtMYB2 (Unigene44371_All) was the only MYB transcription factor，homologous to the MYB transcription factor regulating anthocyanin biosynthesis, expressed in the red tuber epidermis of ‘QY1’. The anthocyanin concentration in the root, stem, leaf, flower, and tuber epidermis of ‘QY1’ was higher than in ‘QY3’, especially tuber epidermis. Correspondingly, HtMYB2 had greater expression in these tissues of ‘QY1’ than in ‘QY3’. The expression of HtMYB2 was associated with anthocyanin accumulation in the different tissues. Overexpression of HtMYB2 activated the anthocyanin biosynthesis pathway, accumulating the pigment in leaves of transgenic tobacco, supporting the model that HtMYB2 regulated anthocyanin biosynthesis. Further experiments found that HtMYB2 had the same coding sequence and genomic sequence in ‘QY1’ and ‘QY3’, but that there were several single nucleotide polymorphisms and one insertion–deletion (indel) mutation of 21 nucleotides in the promoter region between the two alleles. The deletion of three nucleotides “AAA” made the promoter of ‘QY1’ predicted to contain one more possible promoter region. A specific primer, based on the indel, could differentiate between cultivars with red or white tuber epidermis. The genetic variation in HtMYB2 was associated with the tuber skin color in a natural population.Conclusions: RNA-seq can successfully isolate the candidate gene (HTMYB2) controlling anthocyanin biosynthesis in purple epidermis of Jerusalem artichoke tuber. HTMYB2 can regulate anthocyanin biosynthesis in plants and is closely related to the formation of purple phenotype in tubers. This study should be useful in understanding the genetic mechanism underlying different tuber skin colors and in breeding new H. tuberosus cultivars with different tuber skin colors.

show abstract

A functional homologue of Arabidopsis TTG1 from Freesia interacts with bHLH proteins to regulate anthocyanin and proanthocyanidin biosynthesis in both Freesia hybrida and Arabidopsis thaliana

Cited by 51 publications

References 63 publications

MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida

MYB repressors and MBW activation complex collaborate to fine-tune flower coloration in Freesia hybrida

Functional MYB transcription factor gene HtMYB2 is associated with anthocyanin biosynthesis in Helianthus tuberosus L

Article Functional MYB transcription factor gene HtMYB2 is associated with anthocyanin biosynthesis in Helianthus tuberosus L.

Contact Info

Product

Resources

About