A Functional MicroRNA-155 Ortholog Encoded by the Oncogenic Marek's Disease Virus

Zhao, Yuguang; Yao, Yongxiu; Xu, Huji; Lambeth, Luke S.; Smith, Lorraine P.; Kgosana, Lydia; Wang, Xiaowei; Nair, Venugopal

doi:10.1128/jvi.01166-08

Cited by 135 publications

(127 citation statements)

References 39 publications

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“…Intriguingly, KSHV, a related oncogenic g-herpesvirus, encodes a viral miRNA, miR-K11, that not only has the same seed as miR-155, but also targets many of the same cellular mRNAs for down-regulation (Gottwein et al 2007;Skalsky et al 2007). Moreover, the oncogenic chicken a-herpesvirus virus Marek's disease virus type 1 (MDV-1) also encodes a miR-155 ortholog, while MDV-2, a related nononcogenic virus, does not (Zhao et al 2009). Together, these data suggest that miR-155 and its viral homologs play a significant role in some aspect of the life cycle of these diverse herpesviruses, presumably by downregulating cellular mRNAs that remain to be identified.…”

Section: Viral Induction Of Cellular Mirnasmentioning

confidence: 99%

The role of RNAi and microRNAs in animal virus replication and antiviral immunity

Umbach¹,

Cullen²

2009

Genes Dev.

352

324

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The closely related microRNA (miRNA) and RNAi pathways have emerged as important regulators of virus–host cell interactions. Although both pathways are relatively well conserved all the way from plants to invertebrates to mammals, there are important differences between these systems. A more complete understanding of these differences will be required to fully appreciate the relationship between these diverse host organisms and the various viruses that infect them. Insights derived from this research will facilitate a better understanding of viral pathogenesis and the host innate immune response to viral infection.

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Section: Viral Induction Of Cellular Mirnasmentioning

confidence: 99%

The role of RNAi and microRNAs in animal virus replication and antiviral immunity

Umbach¹,

Cullen²

2009

Genes Dev.

352

324

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“…miR-204 and miR-211 directly repress PPM1K expression via 3'UTR To investigate whether PPM1K 3'UTR is a direct target of miR-204 and miR-211, we cloned mouse Ppm1k 3'UTR (4216 base pairs) and human PPM1K 3'UTR (2214 base pairs) fragments into the reporter vector psiCHECK_2, where Renilla luciferase expression can be regulated by a downstream 3'UTR [31] , and generated Luc-mouse 3'UTR and Luc-human 3'UTR reporters, respectively. After co-transfecting these reporter vectors with miRNA mimics into cells, the luciferase activity was measured.…”

Section: Luciferase Reporter Assaysmentioning

confidence: 99%

Regulation of PP2Cm expression by miRNA-204/211 and miRNA-22 in mouse and human cells

et al. 2015

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Aim:The mitochondrial targeted 2C-type serine/threonine protein phosphatase (PP2Cm) is encoded by the gene PPM1K and is highly conserved among vertebrates. PP2Cm plays a critical role in branched-chain amino acid catabolism and regulates cell survival. Its expression is dynamically regulated by the nutrient environment and pathological stresses. However, little is known about the molecular mechanism underlying the regulation of PPM1K gene expression. In this study, we aimed to reveal how PPM1K expression is affected by miRNA-mediated post-transcriptional regulation. Methods: Computational analysis based on conserved miRNA binding motifs was applied to predict the candidate miRNAs that potentially affect PPM1K expression. Dual-luciferase reporter assay was performed to verify the miRNAs' binding sites in the PPM1K gene and their influence on PPM1K 3'UTR activity. We further over-expressed the mimics of these miRNAs in human and mouse cells to examine whether miRNAs affected the mRNA level of PPM1K. Results: Computational analysis identified numerous miRNAs potentially targeting PPM1K. Luciferase reporter assays demonstrated that the 3'UTR of PPM1K gene contained the recognition sites of miR-204 and miR-211. Overexpression of these miRNAs in human and mouse cells diminished the 3'UTR activity and the endogenous mRNA level of PPM1K. However, the miR-22 binding site was found only in human and not mouse PPM1K 3'UTR. Accordingly, PPM1K 3'UTR activity was suppressed by miR-22 overexpression in human but not mouse cells. Conclusion: These data suggest that different miRNAs contribute to the regulation of PP2Cm expression in a species-specific manner. miR-204 and miR-211 are efficient in both mouse and human cells, while miR-22 regulates PP2Cm expression only in human cells.

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“…The mechanism of MDV oncogenesis has historically been an important focus of research for virologists (Nair & Kung, 2004). Recently a large number of viral miRNAs have been reported to be encoded in the genomes of all three serotypes of MDV (Burnside et al, 2006;Yao et al, 2007Yao et al, , 2008Yao et al, , 2009Waidner et al, 2009) and several recent studies suggest that some of the MDV-1-encoded miRNAs, such as miR-M4-5p and miR-M32-3p, have possible functions in MDV oncogenesis (Morgan et al, 2008;Lambeth et al, 2009;Zhao et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Expression profiles of microRNAs encoded by the oncogenic Marek's disease virus reveal two distinct expression patterns in vivo during different phases of disease

Luo

Sun

Teng

et al. 2010

Journal of General Virology

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Marek's disease virus (MDV) is a long-recognized oncogenic herpesvirus, which induces lymphoma in its natural host that can be prevented by vaccination. MDV infection provides an excellent biological model for investigating the biology, genetics and immunology of viral oncogenesis. Recently discovered microRNAs (miRNAs) in the MDV genome have been suggested to have regulatory roles during MDV oncogenesis. We have examined the expression profiles of all 22 previously reported miRNAs encoded by MDV-1 in chickens artificially challenged with MDV-GX0101. We found that a subset of the miRNAs was differentially expressed during different phases of the developing disease. These miRNAs show early or late expression during disease progression, accompanied by obvious tissue-specific and differential expression patterns. This temporal and differential tissue distribution suggest that these miRNAs may perform different regulatory roles in switching from latency to lytic replication, immunosupression, neoplastic transformation or other aspects of lymphoma formation. These reported in vivo expression profiles indicate the potentially functional MDV-1-encoded miRNAs that should be selected for further investigation of their functions in MDV oncogenesis.

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A Functional MicroRNA-155 Ortholog Encoded by the Oncogenic Marek's Disease Virus

Cited by 135 publications

References 39 publications

The role of RNAi and microRNAs in animal virus replication and antiviral immunity

The role of RNAi and microRNAs in animal virus replication and antiviral immunity

Regulation of PP2Cm expression by miRNA-204/211 and miRNA-22 in mouse and human cells

Expression profiles of microRNAs encoded by the oncogenic Marek's disease virus reveal two distinct expression patterns in vivo during different phases of disease

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