A functional molecular marker for detecting blister blight disease resistance in tea (Camellia sinensis L.)

Karunarathna, K. H. T.; Mewan, K.M.; Weerasena, O.V.D.S.J.; Perera, S. A. C. N.; Edirisinghe, E.N.U.

doi:10.1007/s00299-020-02637-6

Cited by 18 publications

(12 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PARP-1 is able to promote (directly or indirectly) epigenetic modifications, creating conditions for development of heterogeneity of tumor cells and formation of super-resistant clones in a heterogeneous population [ 64 ]. Another PARP-1-mediated mechanism of drug resistance is a non-lethal autophagy [ 65 , 66 , 67 ]. PARP-1 is also known to control the expression of heat shock protein 70 [ 68 , 69 ], which makes a significant contribution to the survival of tumor cells and their resistance to antitumor agents [ 70 ].…”

Section: Parp-1 and Oncological Diseasesmentioning

confidence: 99%

PARP-1-Associated Pathological Processes: Inhibition by Natural Polyphenols

Feofanov

Studitsky

2021

IJMS

View full text Add to dashboard Cite

Poly (ADP-ribose) polymerase-1 (PARP-1) is a nuclear enzyme involved in processes of cell cycle regulation, DNA repair, transcription, and replication. Hyperactivity of PARP-1 induced by changes in cell homeostasis promotes development of chronic pathological processes leading to cell death during various metabolic disorders, cardiovascular and neurodegenerative diseases. In contrast, tumor growth is accompanied by a moderate activation of PARP-1 that supports survival of tumor cells due to enhancement of DNA lesion repair and resistance to therapy by DNA damaging agents. That is why PARP inhibitors (PARPi) are promising agents for the therapy of tumor and metabolic diseases. A PARPi family is rapidly growing partly due to natural polyphenols discovered among plant secondary metabolites. This review describes mechanisms of PARP-1 participation in the development of various pathologies, analyzes multiple PARP-dependent pathways of cell degeneration and death, and discusses representative plant polyphenols, which can inhibit PARP-1 directly or suppress unwanted PARP-dependent cellular processes.

show abstract

Section: Parp-1 and Oncological Diseasesmentioning

confidence: 99%

PARP-1-Associated Pathological Processes: Inhibition by Natural Polyphenols

Feofanov

Studitsky

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…It has the advantages of good reproducibility, codominance, abundant polymorphisms, and easy detection [ 20 ]. In recent years, SSR markers have been widely used in genetic diversity analysis [ 21 , 22 ], linkage genetic map construction and QTL identification [ 23 , 24 ], and marker-assisted breeding [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

De novo transcriptome assembly, gene annotation, and EST-SSR marker development of an important medicinal and edible crop, Amomum tsaoko (Zingiberaceae)

Meng

Lei

et al. 2022

BMC Plant Biol

View full text Add to dashboard Cite

Background Amomum tsaoko is a medicinal and food dual-use crop that belongs to the Zingiberaceae family. However, the lack of transcriptomic and genomic information has limited the understanding of the genetic basis of this species. Here, we performed transcriptome sequencing of samples from different A. tsaoko tissues, and identified and characterized the expressed sequence tag-simple sequence repeat (EST-SSR) markers. Results A total of 58,278,226 high-quality clean reads were obtained and de novo assembled to generate 146,911 unigenes with an N50 length of 2002 bp. A total of 128,174 unigenes were successfully annotated by searching seven protein databases, and 496 unigenes were identified as annotated as putative terpenoid biosynthesis-related genes. Furthermore, a total of 55,590 EST-SSR loci were detected, and 42,333 primer pairs were successfully designed. We randomly selected 80 primer pairs to validate their polymorphism in A. tsaoko; 18 of these primer pairs produced distinct, clear, and reproducible polymorphisms. A total of 98 bands and 96 polymorphic bands were amplified by 18 pairs of EST-SSR primers for the 72 A. tsaoko accessions. The Shannon's information index (I) ranged from 0.477 (AM208) to 1.701 (AM242) with an average of 1.183, and the polymorphism information content (PIC) ranged from 0.223 (AM208) to 0.779 (AM247) with an average of 0.580, indicating that these markers had a high level of polymorphism. Analysis of molecular variance (AMOVA) indicated relatively low genetic differentiation among the six A. tsaoko populations. Cross-species amplification showed that 14 of the 18 EST-SSR primer pairs have transferability between 11 Zingiberaceae species. Conclusions Our study is the first to provide transcriptome data of this important medicinal and edible crop, and these newly developed EST-SSR markers are a very efficient tool for germplasm evaluation, genetic diversity, and molecular marker-assisted selection in A. tsaoko.

show abstract

“…In the last 15 years, several SSRs markers have been developed from microRNA (miRNA), mRNA, genome, and chloroplast sequences to study the genetic variation and population structure in diferent genera of Camellia , such as C. sinensis, C. osmanthus, C. vietnamensis, C. gauchowensis, C. huana, C. sasanqua, C. oleifera, C. japonica, and C. reticulata. In the last three years, SSR markers in the genus Camellia have emerged as a highly interesting research topic, with at least 14 studies on SSR markers [28][29][30][31][32][33][34][35][36][37][38][39][40][41], including both genome-wide SSR markers and SSR identifcation of single resistance genes, gene families, whole transcription factors, and the development of SSR databases. For example, an SSR marker was used as a molecular marker to tag the blister blight disease-resistance trait of C. sinensis [29,35].…”

Section: Introductionmentioning

confidence: 99%

“…In the last three years, SSR markers in the genus Camellia have emerged as a highly interesting research topic, with at least 14 studies on SSR markers [28][29][30][31][32][33][34][35][36][37][38][39][40][41], including both genome-wide SSR markers and SSR identifcation of single resistance genes, gene families, whole transcription factors, and the development of SSR databases. For example, an SSR marker was used as a molecular marker to tag the blister blight disease-resistance trait of C. sinensis [29,35]. Similarly, 72 SSR loci were detected in 14 and 15 phospholipase D gene families of C. sinensis for marker-assisted selection of resistance genes [37].…”

Section: Introductionmentioning

confidence: 99%

Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae)

Bai

Yang

et al. 2022

Genetics Research

View full text Add to dashboard Cite

Camellia nitidissima Chi (CNC), a species of golden Camellia, is well known as “the queen of camellias.” It is an ornamental, medicinal, and edible plant grown in China. In this study, we conducted a genome survey sequencing analysis and simple sequence repeat (SSR) identification of CNC using the Illumina sequencing platform. The 21-mer analysis predicted its genome size to be 2,778.82 Mb, with heterozygosity and repetition rates of 1.42% and 65.27%, respectively. The CNC genome sequences were assembled into 9,399,197 scaffolds, covering ∼2,910 Mb and an N50 of 869 base pair. Its genomic characteristics were found to be similar to those of Camellia oleifera. In addition, 1,940,616 SSRs were identified from the genome data, including mono-(61.85%), di-(28.71%), tri-(6.51%), tetra-(1.85%), penta-(0.57%), and hexanucleotide motifs (0.51%). We believe these data will provide a useful foundation for the development of novel molecular markers for CNC as well as for further whole-genome sequencing of CNC.

show abstract

A functional molecular marker for detecting blister blight disease resistance in tea (Camellia sinensis L.)

Cited by 18 publications

References 20 publications

PARP-1-Associated Pathological Processes: Inhibition by Natural Polyphenols

PARP-1-Associated Pathological Processes: Inhibition by Natural Polyphenols

De novo transcriptome assembly, gene annotation, and EST-SSR marker development of an important medicinal and edible crop, Amomum tsaoko (Zingiberaceae)

Genome Survey and SSR Analysis of Camellia nitidissima Chi (Theaceae)

Contact Info

Product

Resources

About