A functional neuron maturation device provides convenient application on microelectrode array for neural network measurement

Han, Xiaobo; Matsuda, Naoki; Ishibashi, Yasuyuki; Odawara, Aoi; Takahashi, Sayuri; Tooi, Norie; Kinoshita, Koshi; Suzuki, I.

doi:10.1186/s40824-022-00324-z

Cited by 6 publications

(2 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that the development of ultra-sensitive neurotransmitter biosensors with sensitivity at the levels of ≤ nano units is meaningful for early disease diagnosis and monitoring [ 15 , 16 , 17 , 18 , 19 ]. In this context, the fusion of biosensors and biomaterials enhances the potential for sensing specific biomolecules [ 20 , 21 , 22 , 23 , 24 , 25 ]. In particular, biocompatible molecules, such as DNA- and RNA-based nucleic acids, are highly beneficial for the sensitive and selective detection of analytes.…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

2023

View full text Add to dashboard Cite

In recent years, there has been an increased demand for highly sensitive and selective biosensors for neurotransmitters, owing to advancements in science and technology. Real-time sensing is crucial for effective prevention of neurological and cardiovascular diseases. In this review, we summarise the latest progress in aptamer-based biosensor technology, which offers the aforementioned advantages. Our focus is on various biomaterials utilised to ensure the optimal performance and high selectivity of aptamer-based biosensors. Overall, this review aims to further aptamer-based biosensor technology.

show abstract

Section: Introductionmentioning

confidence: 99%

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

2023

View full text Add to dashboard Cite

show abstract

“…These materials need to possess mechanical properties and biochemical cues that support the functional maturation of 3D neuronal networks [14,15]. Consequently, there is a need for a material that can support long-term cultures, especially those based on 3D neuronal networks derived from h-iPSCs, as achieving functional maturation of induced neurons (iNeurons) in 3D settings requires a relatively long time (6 weeks or more) [16,17], necessitating thus a stable biomaterial [18]. Hydrogels are widely used biomaterials for mimicking the brain's ECM [19,20].…”

Section: Introductionmentioning

confidence: 99%

Long-term in vitro culture of 3D brain tissue model based on chitosan thermogel

Lisa,

Muzzi,

Lagazzo

et al. 2023

Biofabrication

View full text Add to dashboard Cite

Methods for studying brain function and disease heavily rely on in vivo animal models, ex-vivo tissue slices, and 2D cell culture platforms. These methods all have limitations that significantly impact the clinical translatability of results. Consequently, models able to better recapitulate some aspects of in vivo human brain are needed as additional preclinical tools. In this context, 3D hydrogel-based in vitro models of the brain are considered promising tools. To create a 3D brain-on-a-chip model, a hydrogel capable of sustaining neuronal maturation over extended culture periods is required. Among biopolymeric hydrogels, chitosan-β-glycerophosphate (CHITO-β-GP) thermogels have demonstrated their versatility and applicability in the biomedical field over the years. In this study, we investigated the ability of this thermogel to encapsulate neuronal cells and support the functional maturation of a 3D neuronal network in long-term cultures. To the best of our knowledge, we demonstrated for the first time that CHITO-β-GP thermogel possesses optimal characteristics for promoting neuronal growth and the development of an electrophysiologically functional neuronal network derived from both primary rat neurons and neurons differentiated from human induced pluripotent stem cells (h-iPSCs) co-cultured with astrocytes. Specifically, two different formulations were firstly characterized by rheological, mechanical and injectability tests. Primary nervous cells and neurons differentiated from h-iPSCs were embedded into the two thermogel formulations. The 3D cultures were then deeply characterized by immunocytochemistry, confocal microscopy, and electrophysiological recordings, employing both 2D and 3D micro-electrode arrays. The thermogels supported the long-term culture of neuronal networks for up to 100 d. In conclusion, CHITO-β-GP thermogels exhibit excellent mechanical properties, stability over time under culture conditions, and bioactivity toward nervous cells. Therefore, they are excellent candidates as artificial extracellular matrices in brain-on-a-chip models, with applications in neurodegenerative disease modeling, drug screening, and neurotoxicity evaluation.

show abstract

Decoding Epileptic Seizures: Exploring In Vitro Approaches to Unravel Pathophysiology and Propel Future Therapeutic Breakthroughs

Heydari,

Bozzi,

Pavesi

2024

Biomedical Materials & Devices

View full text Add to dashboard Cite

Epilepsy is a chronic neurological disorder associated with various symptoms, contingent upon the specific brain region involved. Unpredictable seizures characterize epilepsy, significantly influencing the quality of the patient’s life. Globally, epilepsy affects 1% of the population, with 30% of individuals developing drug resistant epilepsy despite anti-epileptic pharmacological treatment. While several anticonvulsant drugs alleviate epilepsy symptoms, there is currently no effective medication to cure this neurological disorder. Therefore, overcoming the challenges of predicting and controlling drug-resistant seizures requires further knowledge of the pathophysiology of epilepsy at the molecular and cellular levels. In this review, we delve into in vitro experiments that prove valuable in elucidating the mechanisms of drug-resistant epilepsy, as well as in the development and testing of novel therapeutic approaches prior to extensive animal-based trials. Specifically, our focus is on the utility of multi-electrode array (MEA) recording as an in vitro technique for evaluating aberrant electrical activity within neural networks. Real-time MEA recording from neuronal cultures facilitates monitoring of neurotoxicity, dose response, and the efficacy of newly-designed drugs. Additionally, when coupled with emerging techniques such as optogenetics, MEA enables the creation of closed-loop systems for seizure prediction and modulation. These integrated systems contribute to both prospective therapy and the study of intracellular pathways in drug-resistant seizures, shedding light on their impact on neuronal network activity.

show abstract

A functional neuron maturation device provides convenient application on microelectrode array for neural network measurement

Cited by 6 publications

References 57 publications

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

Recent Advances in Aptamer-Based Sensors for Sensitive Detection of Neurotransmitters

Long-term in vitro culture of 3D brain tissue model based on chitosan thermogel

Decoding Epileptic Seizures: Exploring In Vitro Approaches to Unravel Pathophysiology and Propel Future Therapeutic Breakthroughs

Contact Info

Product

Resources

About