A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction

Wang, Fu-Peng; Guan, Yan; Liu, Jia-Wen; Cheng, Huan; Hu, Rong

doi:10.1039/d3an01122d

Cited by 1 publication

(1 citation statement)

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“…27−30 Especially, HCR-assembled DNA nanowire has attracted researchers' attention in low-expressed RNA imaging owing to its simple preparation and stable structure. 31,32 resonance energy transfer and HCR to construct a DNA nanowire for visualization of cancer-related mRNA in living cells. 33 Jiang et al developed an electrostatic DNA nanoassembly in which target mRNA initiated HCR to produce long DNA nanowires for efficient intracellular mRNA imaging.…”

Section: ■ Introductionmentioning

confidence: 99%

Dual-Function DNA Nanowires with Self-Feedback Amplification and Efficient Signal Transduction for Intracellular Imaging of MicroRNA-155

Liu,

Zhao,

Wei

et al. 2024

Anal. Chem.

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Intracellular detection and imaging of microRNAs (miRNAs) with low expression usually face the problem of unsatisfactory sensitivity. Herein, a novel dual-function DNA nanowire (DDN) with self-feedback amplification and efficient signal transduction was developed for the sensitive detection and intracellular imaging of microRNA-155 (miRNA-155). Target miRNA-155 triggered catalytic hairpin assembly (CHA) to generate plenty of double-stranded DNA (dsDNA), and a trigger primer exposed in dsDNA initiated a hybridization chain reaction (HCR) between four well-designed hairpins to produce DDN, which was encoded with massive target sequences and DNAzyme. On the one hand, target sequences in DDN acted as self-feedback amplifiers to reactivate cascaded CHA and HCR, achieving exponential signal amplification. On the other hand, DNAzyme encoded in DDN acted as signal transducers, successively cleaving Cy5 and BHQ-2 labeled substrate S to obtain a significantly enhanced fluorescence signal. This efficient signal transduction coupling self-feedback amplification greatly improved the detection sensitivity with a limit of detection of 160 aM for miRNA-155, enabling ultrasensitive imaging of low-abundance miRNA-155 in living cells. The constructed DDN creates a promising fluorescence detection and intracellular imaging platform for low-expressed biomarkers, exhibiting tremendous potential in biomedical studies and clinical diagnosis of diseases.

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Section: ■ Introductionmentioning

confidence: 99%

Dual-Function DNA Nanowires with Self-Feedback Amplification and Efficient Signal Transduction for Intracellular Imaging of MicroRNA-155

Liu,

Zhao,

Wei

et al. 2024

Anal. Chem.

View full text Add to dashboard Cite

show abstract

A functional nucleic acid-based fluorescence sensing platform based on DNA supersandwich nanowires and cation exchange reaction

Abstract: Accurate and sensitive analysis of p53 DNA is important for early diagnosis of cancer. In this work, a fluorescence sensing system based on DNA supersandwich nanowires and cation exchange (CX)-triggered...

Cited by 1 publication

References 38 publications

Dual-Function DNA Nanowires with Self-Feedback Amplification and Efficient Signal Transduction for Intracellular Imaging of MicroRNA-155

Dual-Function DNA Nanowires with Self-Feedback Amplification and Efficient Signal Transduction for Intracellular Imaging of MicroRNA-155

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