A functional plasmid-borne rrn operon in soil isolates belonging to the genus Paracoccus

Battermann, Anja; Disse-Krömker, Claudia; Dreiseikelmann, Brigitte

doi:10.1099/mic.0.26608-0

Cited by 16 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These are embedded in contigs that do not contain any additional ribosomal components and are only ~90% similar in sequence to the equivalents in their host strains, so that the detection of these informational genes is unlikely to be due to host chromosome contamination. Their presence therefore confirms previous findings that plasmids can occasionally contain rRNA genes ( 40 , 41 ) and can act as transfer vehicles for genes that are thought to be only infrequently involved in exchange among distantly related organisms ( 42 , 43 ).…”

Section: Resultssupporting

confidence: 89%

Eco-Evolutionary Dynamics of Episomes among Ecologically Cohesive Bacterial Populations

Xue

Cordero

Camas

et al. 2015

mBio

View full text Add to dashboard Cite

Although plasmids and other episomes are recognized as key players in horizontal gene transfer among microbes, their diversity and dynamics among ecologically structured host populations in the wild remain poorly understood. Here, we show that natural populations of marine Vibrionaceae bacteria host large numbers of families of episomes, consisting of plasmids and a surprisingly high fraction of plasmid-like temperate phages. Episomes are unevenly distributed among host populations, and contrary to the notion that high-density communities in biofilms act as hot spots of gene transfer, we identified a strong bias for episomes to occur in free-living as opposed to particle-attached cells. Mapping of episomal families onto host phylogeny shows that, with the exception of all phage and a few plasmid families, most are of recent evolutionary origin and appear to have spread rapidly by horizontal transfer. Such high eco-evolutionary turnover is particularly surprising for plasmids that are, based on previously suggested categorization, putatively nontransmissible, indicating that this type of plasmid is indeed frequently transferred by currently unknown mechanisms. Finally, analysis of recent gene transfer among plasmids reveals a network of extensive exchange connecting nearly all episomes. Genes functioning in plasmid transfer and maintenance are frequently exchanged, suggesting that plasmids can be rapidly transformed from one category to another. The broad distribution of episomes among distantly related hosts and the observed promiscuous recombination patterns show how episomes can offer their hosts rapid assembly and dissemination of novel functions.

show abstract

Section: Resultssupporting

confidence: 89%

Eco-Evolutionary Dynamics of Episomes among Ecologically Cohesive Bacterial Populations

Xue

Cordero

Camas

et al. 2015

mBio

View full text Add to dashboard Cite

show abstract

“…Furthermore, rrn copies may be present in more than one chromosome in bacteria that possess two chromosomes, e.g., in Brucella ( Michaux et al, 1993 ) and Vibrio ( Yamaichi et al, 1999 ). In addition, rrn copies are also found in both chromosomes and plasmids as in B. megaterium ( Kunnimalaiyaan et al, 2001 ) or Paracoccus species ( Battermann et al, 2003 ). A special case is Aureimonas sp.…”

Section: Ribosomal Rna Operons In Bacteriamentioning

confidence: 99%

Multiple Ribosomal RNA Operons in Bacteria; Their Concerted Evolution and Potential Consequences on the Rate of Evolution of Their 16S rRNA

Espejo

Plaza

2018

Front. Microbiol.

View full text Add to dashboard Cite

Bacterial species differ greatly in the number and location of the rRNA operons which may be present in the bacterial chromosomes and plasmids. Most bacterial species contain more than one ribosomal RNA operon copy in their genomes, with some species containing up to 15 such copies. We review the number and location of the rRNA operons and discuss evolution of 16S rRNA (rrs) genes -which are considered as ultimate chronometers for phylogenetic classification- in bacteria with multiple copies of these genes. In these bacterial species, the rrs genes must evolve in concert and sequence changes generated by mutation or horizontal gene transfer must be either erased or spread to every gene copy to avoid divergence, as it occurs when they are present in different species. Analysis of polymorphic sites in intra-genomic rrs copies identifies putative conversion events and demonstrates that sequence conversion is patchy and occurs in small conversion tracts. Sequence conversion probably arises by a non-reciprocal transfer between two or more copies where one copy contributes only a small contiguous segment of DNA, whereas the other copy contributes the rest of the genome in a fairly well understood molecular process. Because concerted evolution implies that a mutation in any of the rrs copies is either eliminated or transferred to every rrs gene in the genome, this process should slow their evolution rate relative to that of single copy genes. However, available data on the rrs genes in bacterial genomes do not show a clear relationship between their evolution rates and the number of their copies in the genome.

show abstract

“…At the genus level, compared with WL, RL significantly decreased the relative abundance of Paracoccus , which was closely related to the digestion and absorption of nitrogen and carbon [ 33 ]. Moreover, green light decreased the relative abundances of Blautia , which led to increased intestinal permeability [ 34 ], as well as that of Staphylococcus , which can damage the intestinal epithelial barrier [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress

et al. 2022

View full text Add to dashboard Cite

Environmental light has an important impact on the growth, development and oxidative stress of chicks. Thus, we investigated the effects of colored lights on microbes and explored the molecular mechanism by which external color light information alters the gut microbiota and induces the cell response in vivo. We raised 96 chicks under 400–700 nm white (WL), 660 nm red (RL), 560 nm green (GL) or 480 nm blue light (BL) for 42 days. We used 16S rRNA high-throughput pyrosequencing and gas chromatography to explore the effect of different monochromatic lights on the jejunal microbiota. We used qRT-PCR, western blotting, immunohistochemistry and Elisa to determine the effect of different monochromatic lights on small intestine development and oxidative stress levels. With consistency in the upregulation of antioxidant enzyme ability and anti-inflammatory cytokine level, the 16S rRNA and gas chromatography results showed that BL significantly increased the diversity and richness of the jejunal microbiota and improved the relative abundances of Faecalibacterium, Ruminiclostridium_9 and metabolite butyrate content compared with WL, RL and GL (p < 0.05). In addition, we observed that BL increased the goblet cell numbers, PCNA cell numbers, villus-length-to-crypt-depth (V/C) ratios, ZO-1, Occludin, and Claudin-1 protein expression; decreased permeability; and enhanced the digestion and absorption capacity in the jejunum (p < 0.05). In the in vitro experiment, we found that butyrate promoted chick small intestinal epithelial cell (CIEC) proliferation and inhibited apoptosis (p < 0.05). These responses were abrogated by the Gi inhibitor, PI3K inhibitor or AKT inhibitor, but were mimicked by GPR43 agonists or the GSK-3β inhibitor (p < 0.05). Overall, these findings suggested that BL increased the relative abundance of Faecalibacterium, Ruminiclostridium_9 and butyrate production. Butyrate may act as one of the signals to mediate blue-light-induced small intestinal development and mucosal barrier integrity enhancement and promote cell proliferation via the GPR43/Gi/PI3K/AKT/p-GSK-3β/β-catenin pathway.

show abstract

A functional plasmid-borne rrn operon in soil isolates belonging to the genus Paracoccus

Cited by 16 publications

References 23 publications

Eco-Evolutionary Dynamics of Episomes among Ecologically Cohesive Bacterial Populations

Eco-Evolutionary Dynamics of Episomes among Ecologically Cohesive Bacterial Populations

Multiple Ribosomal RNA Operons in Bacteria; Their Concerted Evolution and Potential Consequences on the Rate of Evolution of Their 16S rRNA

Blue Light Alters the Composition of the Jejunal Microbiota and Promotes the Development of the Small Intestine by Reducing Oxidative Stress

Contact Info

Product

Resources

About