A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation

Wang, Lin-Lin; Zhao, Rui; Liu, Changsheng; Liu, Min; Li, Shanshan; Li, Jiao‐Yong; Jiang, Shenyi; Zhang, Miao; Tian, Zhi-Ling; Wang, Meng; Zhang, Meng-Zhou; Guan, Dawei

doi:10.1016/j.jflm.2016.01.027

Cited by 31 publications

(21 citation statements)

References 34 publications

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“…This reduction in leucocytes was mainly due to a decrease in the blood granulocytes (data not shown). This blood granulocytes reduction may be explained, at least in part, by the expected neutrophils infiltration within the wound healing after the skin incision as based on previous evidence (Wang et al 2016). Hypoglycaemia at the advanced period (approximately 24 h after CLP in mice models) was previously explained by a reduced peripheral response to catecholamines, that is, in the liver (Tang et al 1998), even though circulating catecholamines are found elevated during this period (Hahn et al 1995).…”

Section: Discussionmentioning

confidence: 64%

See 1 more Smart Citation

Glucose homeostasis in two degrees of sepsis lethality induced by caecum ligation and puncture in mice

Ferreira

Santos

Bruxel

et al. 2017

Int J Experimental Path

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Sepsis is associated with high mortality. Both critically ill humans and animal models of sepsis exhibit changes in their glucose homeostasis, that is, hypoglycaemia, with the progression of infection. However, the relationship between basal glycaemia, glucose tolerance and insulin sensitivity is not well understood. Thus, we aimed to evaluate this glucose homeostasis triad at the late stage of sepsis (24 h after surgery) in male Swiss mice subjected to lethal and sublethal sepsis by the caecal ligation and puncture (CLP) model. The percentage of survival 24 h after CLP procedure in the Lethal and Sublethal groups was around 66% and 100% respectively. Both Lethal and Sublethal groups became hypoglycaemic in fasting and fed states 24 h after surgery. The pronounced fed hypoglycaemia in the Lethal group was not due to worsening anorexic behaviour or hepatic inability to deliver glucose in relation to the Sublethal group. Reduction in insulin sensitivity in CLP mice occurred in a lethality-dependent manner and was not associated with glucose intolerance. Analysis of oral and intraperitoneal glucose tolerance tests, as well as the gastrointestinal motility data, indicated that CLP mice had reduced intestinal glucose absorption. Altogether, we suggest cessation of appetite and intestinal glucose malabsorption are key contributors to the hypoglycaemic state observed during experimental severe sepsis.

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Section: Discussionmentioning

confidence: 64%

“…This blood granulocytes reduction may be explained, at least in part, by the expected neutrophils infiltration within the wound healing after the skin incision as based on previous evidence (Wang et al . ).…”

Section: Discussionmentioning

confidence: 97%

Glucose homeostasis in two degrees of sepsis lethality induced by caecum ligation and puncture in mice

Ferreira

Santos

Bruxel

et al. 2017

Int J Experimental Path

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show abstract

“…bFGF has exhibited beneficial effects for reversing regional blood flow and has been shown to improve wound healing in both animal models and clinical studies (Akita, Akino, & Hirano, ; Yun et al, ). KGF, a highly specific cytokine that specifically exerts mitogenic effects on epithelial cells (Chen, Kao, Dong, Jiang, & Guo, ; Werner, ), has been reported to be a key factor in wound healing, as it is weakly expressed in human skin but is strongly upregulated after skin injury (Beer et al, ; Jeschke et al, ; Wang et al, ).…”

Section: Introductionmentioning

confidence: 99%

The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing

Cao

et al. 2018

J Tissue Eng Regen Med

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The major challenges associated with skin regeneration can include hindered vascularization and an insufficient degree of epithelization. In view of the complexity of these processes and the control signals on which they depend, one possible solution to these limitations could be simulating normal skin development and wound repair via the exogenous delivery of multiple cytokines. Here, we report the use of keratinocyte growth factor (KGF or FGF-7) and basic fibroblast growth factor (bFGF or FGF-2) released chemically modified collagen membranes to facilitate skin wound healing. The results from in vitro studies confirmed that this system resulted in higher cellular proliferation and faster cell migration. After transplanting the biomaterial onto an excisional wound healing model, the dual growth factor group, compared with the single growth factor groups and empty control group, showed more highly developed vascular networks and organized epidermal regeneration in the wounds. As a consequence, this experimental group showed mature epidermal coverage. Overall, this novel approach of releasing growth factors from a collagen membrane opens new avenues for fulfilling unmet clinical needs for wound care.

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“…Therefore, in order to obtain a more complete and unequivocal information multiple biomarkers should be used. A recent and interesting study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation (from 12 h to 10 days) was based on neutrophil and macrophage infiltration, fibroblast and fibrocyte accumulation, their myofibroblastic transformation and some wound healing-related molecules [9]. One other classification of biomarkers is based on the number of compounds forming part of the given biomarker.…”

Section: Single and Multiple Biomarkersmentioning

confidence: 99%

“…A number of wound healing-related molecules (e.g., IL-1b, IL-6, TNF-a, IFN-g, MCP-1, CXCL12, VEGF-A, EGF, KGF, pro-col Ia2 and pro-col IIIa1, and also fibroblasts and fibrocyte spartially transformed into contractile myofibroblasts) have provided fundamental information for wound age estimation by comprehensive analysis of these molecules from the perspective of morphometrics, protein and gene expressions. This information can be of great interest in forensic analysis [9]. Transformations of these molecules during the wound healing process undoubtedly produce characteristic metabolites that can provide information on the process by simpler, cheaper and faster analyses.…”

Section: Future Of Metabolomics Biomarkers In Forensicsmentioning

confidence: 99%

Metabolomics in the Context of Forensic Omics Biomarkers

LS¹,

Castro²

2017

IJFP

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A fundamental study on the dynamics of multiple biomarkers in mouse excisional wounds for wound age estimation

Cited by 31 publications

References 34 publications

Glucose homeostasis in two degrees of sepsis lethality induced by caecum ligation and puncture in mice

Glucose homeostasis in two degrees of sepsis lethality induced by caecum ligation and puncture in mice

The dual delivery of KGF and bFGF by collagen membrane to promote skin wound healing

Metabolomics in the Context of Forensic Omics Biomarkers

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