A fungal elicitor enhances the resistance of tomato fruit to Fusarium oxysporum infection by activating the phenylpropanoid metabolic pathway

Troncoso‐Rojas, Rosalba; Sánchez‐Estrada, Alberto; Carvallo, Tania; González-León, Alberto; Ojeda-Contreras, Javier; Aguilar-Valenzuela, Alfonso; Tiznado-Hernández, Martín-Ernesto

doi:10.1007/s12600-012-0271-z

Cited by 22 publications

(16 citation statements)

References 35 publications

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“…Our integrative approach also facilitated the identification of genes and metabolites putatively promoting B. cinerea resistance, and highlighted the role of phenylpropanoids in fruit defence. While the function of phenylpropanoids in tomato fruit response to fungal infection has been previously reported 39,[40][41]42 , the results here suggest that a very specific set of flavonoids is associated with resistance. The naringenin-7-O-glucoside, reported previously by Troncoso-Rojas et al 41 as highly induced upon powdery mildew infection, accumulated in the sensitive lines, while its aglycone naringenin was abundant in the resistant lines.…”

Section: Discussionsupporting

confidence: 55%

“…While the function of phenylpropanoids in tomato fruit response to fungal infection has been previously reported 39,[40][41]42 , the results here suggest that a very specific set of flavonoids is associated with resistance. The naringenin-7-O-glucoside, reported previously by Troncoso-Rojas et al 41 as highly induced upon powdery mildew infection, accumulated in the sensitive lines, while its aglycone naringenin was abundant in the resistant lines. Rutin on the other hand, one of the most abundant flavonoids in tomato reported earlier to accumulate in response to Alternaria solani infection 42 , did not correlate with resistance to B. cinerea infection in our study.…”

Section: Discussionsupporting

confidence: 55%

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Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response

et al. 2020

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Wild tomato species represent a rich gene pool for numerous desirable traits lost during domestication.Here, we exploited an introgression population representing wild desert-adapted species and a domesticated cultivar to establish the genetic basis of gene expression and chemical variation accompanying transfer of wild species-associated fruit traits. Transcriptome and metabolome analysis of 580 lines coupled to pathogen sensitivity assays resulted in the identification of genomic loci significantly linked with levels of hundreds of transcripts and metabolites. These associations occurred in functionally coherent hotspots that represent coordinated perturbation of metabolic pathways and ripening-related processes. We demonstrated the efficacy of our approach by discovering yet undisclosed components of the renowned Solanum alkaloids pathway, identifying new genes and metabolites involved in pathogen defence and linking fungal resistance with changes in the fruit ripening regulatory network. Together, our results outline a framework for understanding metabolism and pathogen resistance during tomato fruit ripening and provide insights into key fruit quality traits.

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Section: Discussionsupporting

confidence: 55%

Section: Discussionsupporting

confidence: 55%

Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response

et al. 2020

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“…The identification and quantification of phenolic compounds were determined according to modified methods described by Mradu et al (2012) and Troncoso-Rojas et al (2013). A 1290 Infinity HPLC system (Agilent Technologies, Santa Clara, California, USA) was used equipped with a quaternary pump and a diode array detector.…”

Section: Hplc Analysismentioning

confidence: 99%

Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance

Tzintzun-Camacho

Hernández-Jiménez

González-Mendoza

et al. 2021

Chil. j. agric. res.

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Winemaking waste contain a high number of bioactive compounds with antimicrobial properties that can be exploited in agriculture. In the present study, hydrolysates from three wine grape (Vitis vinifera L.) marcs were characterized and their antifungal activities against phytopathogenic fungi (Fusarium oxysporum and Alternaria spp.) were evaluated. Wine grape marcs (red, pink and white wine) collected from Ensenada, Baja California, Mexico, were subjected to an acid hydrolysis treatment. Skin hydrolysates of pink and white marcs obtained high concentrations of reducing sugars (5.8 ± 0.1 and 5.5 ± 0.2 g L -1 , respectively). Meanwhile, the highest concentration of total sugars was obtained for skin hydrolysates of white marc (9.7 ± 0.08 g L -1 ). The seed hydrolysates of white marc obtained high concentrations of phenolic compounds (0.52 ± 0.1 mg mL -1 ). In addition, the highest antioxidant activity was found for skin hydrolysates of red marc (96 ± 0.61%). Results of in vitro antifungal assays clearly indicated a marked inhibition of the mycelial growth and spore viability of F. oxysporum (100% inhibition using red and white hydrolysates) rather than Alternaria spp. (58% inhibition exposed to pink hydrolysates), due to high concentration of phenols. According to HPLC analysis, phenolic acids such as gallic acid, hydroxybenzoic acid, vanillic acid and p-coumaric acid were predominant in the hydrolysates. This study demonstrated that the grape marc hydrolysates exhibit a potential antifungal activity, and highlights that the hydrolysates can be exploited in agriculture as a safe alternative of antifungal agents.

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“…Therefore, the resistance induction for controlling carnation vascular wilt has attracted the recent attention of the scientific community, considering the positive environmental impact that its application would have on carnation production [ 17 ]. In other F. oxysporum ( Fox ) - hosting species, such as melon [ 18 ] and tomato [ 19 ], the application of a Fox -derived crude extract generated tissue sensitization and symptom reduction during a subsequent pathogen exposition. Therefore, it has been proposed that these fungal-derived extracts should contain bioactive molecules with defense-eliciting activity [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Mycelium Dispersion from Fusarium oxysporum f. sp. dianthi Elicits a Reduction of Wilt Severity and Influences Phenolic Profiles of Carnation (Dianthus caryophyllus L.) Roots

Santos-Rodríguez

Coy-Barrera

Ardila

2021

Plants

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The fungal pathogen Fusarium oxysporum f. sp. dianthi (Fod) is the causal agent of the vascular wilt of carnation (Dianthus caryophyllus L.) and the most prevalent pathogen in the areas where this flower is grown. For this reason, the development of new control strategies against Fod in carnation has been continuously encouraged, in particular those based on the implementation of plant resistance inducers that can trigger defensive responses to reduce the disease incidence, even at lower economical and environmental cost. In the present study, the effect of the soil supplementation of a biotic elicitor (i.e., ultrasound-assisted dispersion obtained from Fod mycelium) on disease severity and phenolic-based profiles of roots over two carnation cultivars was evaluated. Results suggest that the tested biotic elicitor, namely, eFod, substantially reduced the progress of vascular wilting in a susceptible cultivar (i.e., ‘Mizuki’) after two independent in vivo tests. The LC-MS-derived semi-quantitative levels of phenolic compounds in roots were also affected by eFod, since particular anthranilate derivatives, conjugated benzoic acids, and glycosylated flavonols were upregulated by elicitation after 144 and 240 h post eFod addition. Our findings indicate that the soil-applied eFod has an effect as a resistance inducer, promoting a disease severity reduction and accumulation of particular phenolic-like compounds.

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A fungal elicitor enhances the resistance of tomato fruit to Fusarium oxysporum infection by activating the phenylpropanoid metabolic pathway

Cited by 22 publications

References 35 publications

Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response

Analysis of wild tomato introgression lines elucidates the genetic basis of transcriptome and metabolome variation underlying fruit traits and pathogen response

Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance

Mycelium Dispersion from Fusarium oxysporum f. sp. dianthi Elicits a Reduction of Wilt Severity and Influences Phenolic Profiles of Carnation (Dianthus caryophyllus L.) Roots

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