A Fungal α-Galactosidase from Tricholoma matsutake with Broad Substrate Specificity and Good Hydrolytic Activity on Raffinose Family Oligosaccharides

Abstract

An acidic α-galactosidase designated as TMG was purified from the fruiting bodies The purification protocol entailed ion exchange chromatography on Q-Sepharose and of Tricholoma matsutake with 136-fold purification and a specific activity of 909 units/mg. Mono-Q and fast protein liquid chromatography on Superdex 75. TMG is a monomeric protein exhibiting a molecular mass of 47 kDa in SDS-PAGE and gel filtration. The purified enzyme was identified by LC-MS/MS and three inner amino acid sequences were obtained. The optimum pH and temperature for TMG with pNPGal as substrate were pH 4.5 and 55 °C, respectively. The α-galactosidase activity was strongly inhibited by K

A Tricholoma matsutake Peptide with Angiotensin Converting Enzyme Inhibitory and Antioxidative Activities and Antihypertensive Effects in Spontaneously Hypertensive Rats

“…The fruiting body of the pine mushroom is commercially important in the Far East as a valuable food, not only because of its attractive flavor but also because of the medicinal effects 18 . Many bioactive molecules have been isolated from T. matsutake such as nuclease 19 , polysaccharide 20 , laccase 21 and α-galactosidase 22 . These substances with diverse biological activities are beneficial to human health and are useful in environmental protection.…”

Influence of chemical reagents and UV irradiation on the activity of Penicillium canescens ?-galactosidase

“…the enzyme by silver and mercury ions evidences for the important role of both sulfhydryl and carboxyl and imidazole groups in activity of P. canescens α-galactosidase. Inactivation of enzymes by the silver and mercury ions is shown for a great many of the studied α-galactosidases [5][6][7][8]13], and herewith most of them demonstrate resistance to the effect of p-ChMB and N-ethylmaleimide [6,7]. Along with the chemical modification, which permits revealing catalytically significant residues of amino acids, the investigations of the effect of physical denaturating factors, including UV-effect, permit establishing the limits of mobility of a protein molecule and revealing the groups responsible both for the display of functional properties and for the preservation of active conformation.…”

“…Nowadays α-galactosidases have been found in a broad range of microorganisms and divided, as based on the structure of the catalytic domain and amino acid sequences, into 7 families (GH4, GH27, GH31, GH36, GH57, GH97, GH110) due to hierarchic classification of glycosyl hydrolases (http:// www.cazy.org/). Various forms of α-galactosidases are described: monomeric enzymes of thermophylic bacteria and tricholoma matsutake [5,6], dimeric Bacillus coagulans α-galactosidase [7], homotrimers of Bacillus megaterium [8], tetramers of Bacillus stearothermophilus [9] and Aspergillus nidulans [10]. In accordance with modern notions [11], the enzymatic hydrolysis with glycosidases is realized as a result of consistent actions of the basic components: nucleophilic agent, activating water and electrophilic activator of the reaction center.…”

“…It is noted (http://www.cazy.org/) that residues of asparaginic acid (GH27, GH31, GH36) and residues of glutamic acid (GH57, GH97) may appear as a donor of protons and nucleophiles. The importance of tryptophan (tricholoma matsutake), methionine, cysteine (Trichoderma reesei) and histidine (Cladosporium cladosporioides) residues [6,12,13] for the manifestation of catalytic properties of α-galactosidases has also been shown.…”

Ectomycorrhization of Tricholoma matsutake with Quercus aquifolioides affects the endophytic microbial community of host plant

“…Its fruiting body, referred to as the pine mushroom, is a commercially valuable food that has medicinal effects and an attractive, aromatic odor . Terpenoids, and polysaccharides isolated from the fruiting body of T. matsutake have shown anti‐tumor, antibacterial, and antioxidant activities . Artificial cultivation of T. matsutake has been unsuccessful attributed to its specific growth requirements and the rarity of appropriate forest habitat.…”