For a knot in the 3-sphere, its Upsilon invariant is a continuous, piecewise linear function defined on the interval [0,2]. It is a concordance invariant, so vanishes for slice knots. Since it only changes the sign for the mirror image with reversed orientation, it also vanishes for amphicheiral knots. For alternating knots, more generally, quasi-alternating knots, the Upsilon invariant is determined only by the signature. In particular, it vanishes if such a knot has zero signature. On the other hand, it is known that the Upsilon invariant is a non-zero convex function for L-space knots. In this paper, we construct an infinite family of hyperbolic knots, each of which has zero Upsilon invariant, but is chiral, non-slice, non-alternating. To confirm that the Upsilon invariant vanishes, we calculate the full knot Floer complex.