A Further Simplified Procedure for the Detection of Pseudomonas aeruginosa in Contaminated Aqueous Substrata

Abstract

A simple method for the detection of Pseudomonas aeruginosa in bottled water and aqueous pharmaceutical and cosmetic preparations is described. Subsequent to enrichment in Thom et al.'s (1971) nitrofurantoin broth at 42 °C, streaking positive cultures on to King et al.'s (1954) agar with nitrofurantoin added and incubating at 42 °C. Stabbing colonies into single, three 2 cm layer tubes (violet red bile dextrose agar/plain agar/SIM medium) and identifying strains by their non‐fermentative attack on glucose, motility, oxidase reaction and failure to produce H2S or indole.

Isolation and Characterization of Cellulolytic Bacteria from Activated Sludge

“…Purified strains were subjected to morphological and biochemical tests using standard methods (Stanier et al 1966;Skerman 1967). Gram negative isolates were studied according to Mossel et al (1976). Electron microscopy was performed on cells grown on starch agar.…”

Comparison of two methods for assessing the bacteriological condition of the water supply in slaughter houses

“…This procedure therefore allows the assessment of the presence or absence of at least six taxa in the water sample under examination: (i) Enterobacteriaceae as a group, if required examined further for the presence of the coli-aerogenes group ; (ii) Aeromonas (Schubert, 1975;Mossel et al, 1979); (iii) E. coli (Mossel et al, 1980); (iv) Lancefield group D streptococci (Mossel et al, 1978a); (v) Ps. aeruginosa (Mossel et al, 1976); (vi) Gram-negative organisms not currently looked for but yet of occasional significance (Allen et al, 1983).…”