A Fusobacterium mortiferum strain produces a bacteriocin-like substance(s) inhibiting Salmonella enteritidis

Portrait, Véronique; Cottenceau, Gilles; Pons, Anne-Marie

doi:10.1046/j.1365-2672.2000.00774.x

Cited by 29 publications

(24 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is also possible that this trend illustrates that there is a degree of active selection for ampicillin-resistant E. coli within the growing host. Such active selection of resistant strains could be due to either interbacterial competition causing alterations in the population structure of the enteric flora (6,24) or the direct result of bacterial-host communication (28). Alternatively, the resistance genes themselves may be conferring other properties, besides drug resistance, on those bacteria that carry them.…”

Section: Discussionmentioning

confidence: 99%

Sources of Variation in the Ampicillin-Resistant Escherichia coli Concentration in the Feces of Organic Broiler Chickens

Pleydell

Brown

Woodward

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Currently, there are limited published data for the population dynamics of antimicrobial-resistant commensal bacteria. This study was designed to evaluate both the proportions of the Escherichia coli populations that are resistant to ampicillin at the level of the individual chicken on commercial broiler farms and the feasibility of obtaining repeated measures of fecal E. coli concentrations. Short-term temporal variation in the concentration of fecal E. coli was investigated, and a preliminary assessment was made of potential factors involved in the shedding of high numbers of ampicillin-resistant E. coli by growing birds in the absence of the use of antimicrobial drugs. Multilevel linear regression modeling revealed that the largest component of random variation in log-transformed fecal E. coli concentrations was seen between sampling occasions for individual birds. The incorporation of fixed effects into the model demonstrated that the older, heavier birds in the study were significantly more likely (P ‫؍‬ 0.0003) to shed higher numbers of ampicillin-resistant E. coli. This association between increasing weight and high shedding was not seen for the total fecal E. coli population (P ‫؍‬ 0.71). This implies that, in the absence of the administration of antimicrobial drugs, the proportion of fecal E. coli that was resistant to ampicillin increased as the birds grew. This study has shown that it is possible to collect quantitative microbiological data on broiler farms and that such data could make valuable contributions to risk assessments concerning the transfer of resistant bacteria between animal and human populations.

show abstract

Section: Discussionmentioning

confidence: 99%

Sources of Variation in the Ampicillin-Resistant Escherichia coli Concentration in the Feces of Organic Broiler Chickens

Pleydell

Brown

Woodward

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…For instance, young chickens are very sensitive to enteropathogen infections because their intestinal micro-organisms are not fully established (Nurmi & Rantala, 1973). In 2000, searching for bacteria that produce anti-enteropathogen molecules, Portrait et al (2000) isolated an anaerobic strain (FM1025 T ) from the caecum of a duck. This strain was preliminarily identified as Fusobacterium mortiferum using API galleries.…”

mentioning

confidence: 99%

Megamonas rupellensis sp. nov., an anaerobe isolated from the caecum of a duck

Chevrot

Carlotti²,

Sopena

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

We report here the identification, characterization and culture of a Gram-negative to Gramvariable, rod-shaped, non-spore-forming anaerobic bacterium (strain FM1025 T ) isolated from the caecum of a duck. Phylogenetic analysis based on comparative 16S rRNA gene sequencing showed that this strain clustered with species of the family 'Acidaminococcaceae', with 94.9 % similarity to Megamonas hypermegale DSM 1672 T and less than 91 % similarity with type strains of Pectinatus species. Sequence similarities of at least 98-99 % were observed with numerous sequences deposited in GenBank of uncultured strains from human and chicken caecal contents, but this strain is the first isolate of this taxon to be cultivated and described. On the basis of morphological, physiological and phylogenetic features, this strain should be assigned to a novel species in the genus Megamonas, for which the name Megamonas rupellensis sp. nov. is proposed. The type strain is strain FM1025 T (5DSM 19944 T 5CIP 109788 T ).The microbial composition of the chicken intestine evolves with age and varies in its different sections. It is estimated that 60-90 % of the intestinal bacterial community can not currently be cultivated (Lu et al., 2003). The dominant micro-organisms in the gut include bacteria related to Clostridium, Lactobacillus, Bacteroides and Megamonas (Lan et al., 2002;Zhu et al., 2002). This complex microflora is known to play a key role in the health of birds. For instance, young chickens are very sensitive to enteropathogen infections because their intestinal micro-organisms are not fully established (Nurmi & Rantala, 1973). In 2000, searching for bacteria that produce anti-enteropathogen molecules, Portrait et al. (2000) isolated an anaerobic strain (FM1025 T ) from the caecum of a duck. This strain was preliminarily identified as Fusobacterium mortiferum using API galleries. In the present work, we have characterized this bacterium further and, using 16S rRNA gene sequence analysis, we have shown that strain FM1025 T represents a novel species belonging to the genus Megamonas (Shah & Collins, 1982). Strain FM1025 T was grown routinely at 37 u C on TGY broth (w/v; 3 % tryptone, 2 % yeast extract, 0.5 % glucose, 0.05 % L-cysteine hydrochloride) using either anaerobic jars (AnaeroGen; Oxoid) or an anaerobic chamber containing 10 % H 2 , 10 % CO 2 and 80 % N 2 (MACS DG500; Don Whitley Scientific). Strain FM1025 T was restreaked weekly onto TGY agar [TGY broth solidified with 1.2 % (w/v) agar] and could be stored for several years after mixing 0.5 ml of an overnight culture with 0.5 ml 30 % (w/v) glycerol. Unless otherwise specified, growth of strain FM1025 T was tested in 250 ml TGY broth incubated at 37 u C under continuous stirring (160 r.p.m.) in the anaerobic chamber. Strain FM1025 T grew optimally at 37 uC and the pH range for growth was pH 5-9 with an optimum at pH 7. On solid M63 minimal medium (Miller, 1972) supplemented with the reducing amino acid L-cysteine hydrochloride (0.05 % w/v), no colonies were observed after 72 h ...

show abstract

“…1998). Besides that study, we are unaware of any other investigation on bacteriocin production by fusobacteria except for the report by Portrait et al. (2000).…”

Section: Discussionmentioning

confidence: 99%

Purification and partial characterization of a bacteriocin produced by an oralFusobacterium nucleatumisolate

Ribeiro-Ribas

Carvalho

Vieira

et al. 2009

Journal of Applied Microbiology

View full text Add to dashboard Cite

Aims: To purify and partially characterize a bacteriocin produced by a Fusobacterium nucleatum strain. Methods and Results: Following protein precipitation the effect of different treatments on a bacteriocin produced by a F. nucleatum strain named P12.2 isolated from a patient with periodontitis was evaluated. The antagonistic activity of the intracellular fraction obtained at 80% ammonium sulphate was preserved at pH values from 6·0 to 9·0 and showed to be sensitive to high temperatures and to treatment with proteases. The fraction was submitted to sequential steps of gel filtration, ion exchange, and reverse phase chromatography, and SDS‐PAGE. Data obtained by mass spectrometry revealed that the molecular mass of the protein was 27296 Da. Conclusions: For the first time a bacteriocin produced by a F. nucleatum strain was purified and characterized. Significance and Impact of the Study: This is the first description on characterization of a bacteriocin produced by F. nucleatum. It is possible that the bacteriocin plays a role in the regulation of population levels of periodontopathic organisms.

show abstract

A Fusobacterium mortiferum strain produces a bacteriocin-like substance(s) inhibiting Salmonella enteritidis

Cited by 29 publications

References 11 publications

Sources of Variation in the Ampicillin-Resistant Escherichia coli Concentration in the Feces of Organic Broiler Chickens

Sources of Variation in the Ampicillin-Resistant Escherichia coli Concentration in the Feces of Organic Broiler Chickens

Megamonas rupellensis sp. nov., an anaerobe isolated from the caecum of a duck

Purification and partial characterization of a bacteriocin produced by an oralFusobacterium nucleatumisolate

Contact Info

Product

Resources

About