One of the problems encountered in the design and implementation of a flexible transfer line (FTL) is the layout of the FTL in a restricted area. The layout of the FTL has an important impact on production cost. In this paper we propose efficient FTL layout design procedures for a FTL layout in an asymmetrical and restricted plant area. In order to find the layout of the FTL including the buffer size between each pair of FTL machines, an efficient FTL layout design procedure called a One by One Layout Method (OOLM) in conjunction with genetic algorithm (GA) is proposed. The OOLM generates an efficient solution for a set of irregularly shaped machines through a restricted plant area. A CAD system is linked to the OOLM to draw the FTL layout. The OOLM is not limited to a single static environment, but is highly flexible within the plant structure. An application example was developed, and after a number of operations based on OOLM, an efficient FTL layout design could be found.