This paper proposes a novel secondary frequency regulation technique for an uncertain islanded micro grid (MG). The major motivation of the work is to integrate the intrinsic robustness of the sliding mode control scheme with the disturbance observer to estimate and alleviate the unknown mismatched uncertainties caused by renewable resources and load variations. To this end, a dynamical sliding manifold is first utilized and then a control law is derived with Lyapunov's method which stabilizes the MG dynamics. Moreover, in order to ensure faster time domain responses of the closed-loop system, we employ a power rate reaching law in our proposed control design. Thereafter, the performances of the introduced control strategy are tested on an islanded MG using MATLAB/Simulink, and robustness analysis is also carried out by considering five different case studies. Further, in contrast to the existing approaches such as robust H ∞ and robust PID control, the proposed strategy renders appealing time domain characteristics such as settling time, peak overshoot, and integral absolute frequency error.