A Gammaherpesvirus Establishes Persistent Infection in Neuroblastoma Cells

Cho, Hye Jeong; Song, Mee Hyun

doi:10.14348/molcells.2014.0024

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…HHV4, also known as Epstein-Barr virus (EBV), prevalent in 90 to 95% of the human population, is also clinically associated with various neurological diseases such as primary central nervous system lymphoma, multiple sclerosis, Alzheimer's disease, cerebellar ataxia, and encephalitis (Ali and Lawthom, 2013;Corssmit et al, 1997;Davies et al, 2016;Fallo et al, 2005). Human herpesvirus 8 (HHV8), also known as Kaposi's sarcoma-neurotropic and can infect neuronal cell lines, Sh-Sy5y and Ntera2 cells, as well as human primary neurons (Cho and Song, 2014). MHV-68, also called murid gammaherpesvirus 4, was originally isolated from a bank vole by passage through mouse brain (Terry et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Identification of gammaherpesvirus infection in free-ranging black bears (Ursus americanus)

et al. 2019

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A B S T R A C THerpesvirus infection was investigated in black bears (Ursus americanus) with neurological signs and brain lesions of nonsuppurative encephalitis of unknown cause. Visible cytopathic effects (CPE) could only be observed on days 3-5 post-infection in HrT-18G cell line inoculated with bear tissue extracts. The observed CPE in HrT-18G cells included syncytia, intranuclear inclusions, and cell detachments seen in herpesvirus infection in vitro. Herpesvirus-like particles were observed in viral culture supernatant under the electron microscope, however, capsids ranging from 60 nm to 100 nm in size were often observed in viral cultures within the first two passages of propagation. Herpesvirus infection in the bear tissues and tissue cultures were detected by PCR using degenerate primers specific to the DNA polymerase gene (DPOL) and glycoprotein B gene (gB). DNA sequencing of the amplicon revealed that the detected herpesvirus has 94-95% identity to Ursid gammaherpesvirus 1 (UrHV-1) DNA sequences of DPOL. Phylogenetic analysis of DPOL sequences indicates that black bear herpesviruses and UrHV-1 are closely related and have small distances to members of Rhadinovirus. Interestingly, black bear herpesvirus infections were also found in bears without neurological signs. The DPOL DNA sequence of black bear herpesviruses detected in neurological bears were similar to the those detected in the non-neurological bears. However, the gB DNA sequence detected from the neurological bear is different from non-neurological bear and has only 64.5%-70% identity to each other. It is possible that at least two different types of gammaherpesviruses are present in the U. americanus population or several gammaherpesviruses exist in ursine species. phoma, primary effusion lymphoma in human (Moore et al., 1996;Wong and Damania, 2005). However, both HHV-4 and HHV8 are

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Section: Introductionmentioning

confidence: 99%

Identification of gammaherpesvirus infection in free-ranging black bears (Ursus americanus)

et al. 2019

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“…When the concentration of HDB was 50 µM, the cell growth was significantly inhibited (P<0.01); while at the concentration less than 0.1 µM, HDB did not showsignificant effect on SH-SY5Y cells (P>0.05). SH-SY5Y cells have dopamine-β-hydroxylase activity, acetylcholinergic, glutamatergic and adenosinergic characteristics, so they are often used as in vitro models of neuronal function and differentiation (Cho, Song, 2014;Ma et al, 2015). Meanwhile, SH-SY5Y cells also maintain their cancerous nature (Zhang et al, 2014a), and are often used as a human neuroblastoma cell line for anti-tumor research.…”

Section: Effect Of Hdb On the Proliferation Of Sh-sy5y Cellsmentioning

confidence: 99%

Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract

Tao

Gao

et al. 2017

Braz. J. Pharm. Sci.

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Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra-and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%.

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Differentiated Human SH-SY5Y Cells Provide a Reductionist Model of Herpes Simplex Virus 1 Neurotropism

Shipley

Mangold

Kuny

et al. 2017

J Virol

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Neuron-virus interactions that occur during herpes simplex virus (HSV) infection are not fully understood. Neurons are the site of lifelong latency and are a crucial target for long-term suppressive therapy or viral clearance. A reproducible neuronal model of human origin would facilitate studies of HSV and other neurotropic viruses. Current neuronal models in the herpesvirus field vary widely and have caveats, including incomplete differentiation, nonhuman origins, or the use of dividing cells that have neuropotential but lack neuronal morphology. In this study, we used a robust approach to differentiate human SH-SY5Y neuroblastoma cells over 2.5 weeks, producing a uniform population of mature human neuronal cells. We demonstrate that terminally differentiated SH-SY5Y cells have neuronal morphology and express proteins with subcellular localization indicative of mature neurons. These neuronal cells are able to support a productive HSV-1 infection, with kinetics and overall titers similar to those seen in undifferentiated SH-SY5Y cells and the related SK-N-SH cell line. However, terminally differentiated, neuronal SH-SY5Y cells release significantly less extracellular HSV-1 by 24 h postinfection (hpi), suggesting a unique neuronal response to viral infection. With this model, we are able to distinguish differences in neuronal spread between two strains of HSV-1. We also show expression of the antiviral protein cyclic GMP-AMP synthase (cGAS) in neuronal SH-SY5Y cells, which is the first demonstration of the presence of this protein in nonepithelial cells. These data provide a model for studying neuron-virus interactions at the single-cell level as well as via bulk biochemistry and will be advantageous for the study of neurotropic viruses Herpes simplex virus (HSV) affects millions of people worldwide, causing painful oral and genital lesions, in addition to a multitude of more severe symptoms such as eye disease, neonatal infection, and, in rare cases, encephalitis. Presently, there is no cure available to treat those infected or prevent future transmission. Due to the ability of HSV to cause a persistent, lifelong infection in the peripheral nervous system, the virus remains within the host for life. To better understand the basis of virus-neuron interactions that allow HSV to persist within the host peripheral nervous system, improved neuronal models are required. Here we describe a cost-effective and scalable human neuronal model system that can be used to study many neurotropic viruses, such as HSV, Zika virus, dengue virus, and rabies virus.

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A Gammaherpesvirus Establishes Persistent Infection in Neuroblastoma Cells

Cited by 3 publications

References 24 publications

Identification of gammaherpesvirus infection in free-ranging black bears (Ursus americanus)

Identification of gammaherpesvirus infection in free-ranging black bears (Ursus americanus)

Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract

Differentiated Human SH-SY5Y Cells Provide a Reductionist Model of Herpes Simplex Virus 1 Neurotropism

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