Difficulties in direct monitoring of nitrate balance in agricultural fields reveal the importance of modeling and quantifying the affecting parameters on nitrate balance. We constructed meta-models for APSIMX-Sugarcane using the treed gaussian process and conducted a global sensitivity analysis for nitrate uptake and leaching under three conditions: (1) bare land (BL) to examine the influence of soil hydraulic characteristics, (2) N-free treatment under radiation use efficiency (RUE) ranges (i) 1.2–1.8 [N-free(a)] and (ii) 1.8–2.5 [N-free(b)], and (3) urea conditions to examine the influence of plant growth. Generated meta-models showed good accuracy (for all conditions: R2 > 0.70; NRMSE < 16%; AI > 0.90). The most influential parameters (sensitivity indices ≥ 0.02) were as follows: for leached NO3−N in BL: the parameter rerated to saturated flow-proportion of water between saturation and field capacity (SWCON) of all soil layers; for NO3− uptake and leached NO3−N in N-free(a) and urea: RUE of the phenological stage (PS) 3 (RUE3) and 4, tt_emerg_to_begcane, green_leaf_no, and y_n_conc_crit_leaf of PS 4 (NCL4); in N-free(b): RUE3, NCL4, and SWCON of soil layers 0–15 cm; 15–30 cm, which confirmed that influential parameters were depended on N-stress. The outcomes of this study are useful for enhancing the accuracy and efficiency of crop modeling.