A gE deleted infectious bovine rhinotracheitis marker vaccine for use in improved bovine herpesvirus 1 control programs

Strube, W.; Auer, S.; Block, Wolfgang; Heinen, Ernst; Kretzdorn, D.; Rodenbach, C; Schmeer, N.

doi:10.1016/s0378-1135(96)01246-1

Cited by 49 publications

(36 citation statements)

References 5 publications

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“…Others have shown that gE -bovine herpesviruses may be suitable vaccine viruses in differential vaccines (1,14). Although BoHV isolates are regarded as stable, small antigenic variations may occur and it is possible that autochtonous isolates are more antigenically representative of viruses that are affecting a specific geografical region.…”

Section: Discussionmentioning

confidence: 99%

“…Glycoprotein E is not essential for viral replication, but in vitro growth analysis of viral mutants show a decreased cell-to-cell spread and smaller plaque size for gE negative viruses (11,17). In addition, gE deletion in vivo has been related to a decreased virulence in calves (1,14,15).…”

Section: Introductionmentioning

confidence: 99%

“…An european gE negative (gE -) BoHV-1.1 strain has been used as a differential vaccine for the control of BoHV-1 infections, allowing the differentiation between the immune responses of infected and vaccinated animals (1,14,15). Although BoHV-1 isolates are genetically stable, small antigenic variations may occur.…”

Section: Introductionmentioning

confidence: 99%

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Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil

Franco¹,

Rijsewijk²,

Flores³

et al. 2002

Braz. J. Microbiol.

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This paper describes the construction and characterization of a Brazilian strain of bovine herpesvirus type 1.2a (BoHV-1.2a) with a deletion of the glycoprotein E (gE) gene. The deletion was introduced by cotransfection of a deletion fragment containing the 5´and 3´gE flanking regions and genomic DNA of wild type BoHV-1 into bovine cells. Isolation of gE deletion mutant was performed by immunoperoxidase staining with an anti-gE monoclonal antibody. Viral clones were plaque purified and further examined by restriction endonuclesase digestion and Southern blot hybridization. This gE deletion mutant will be evaluated as a vaccinal virus, in order to determine its potential use for a differential vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil

Franco¹,

Rijsewijk²,

Flores³

et al. 2002

Braz. J. Microbiol.

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“…This correlation may explain the significant difference that was found between 1996 and 1998 in the number of positive dams and the relatively higher risk of calves being P or PP in 1998 (Table 4 and 5). In the beginning of 1998, a compulsory eradication programme for BHV1 was started in the Netherlands, using a gE-negative marker vaccine (10). In this programme entire herds (BHV1 positive as well as negative animals) are vaccinated.…”

Section: Discussionmentioning

confidence: 99%

“…Of the 55 other herds, it was known that they had been infected in the past by a BHV1 field virus. All dams of these infected herds were vaccinated with a marker vaccine that lacks the gE-glycoprotein (10). Because of the infection and/or vaccination, all of these dams were positive in at least one of the serological tests used in this investigation (Table 1).…”

Section: Animalsmentioning

confidence: 99%

The serological BHV1 status of dams determines the precolostral status of their calves

Bosch¹,

Lieshout²,

Wit

et al. 2000

Veterinary Quarterly

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BHV-1: New Molecular Approaches to Control a Common and Widespread Infection

Turin

Russo

Poli

1999

Mol Med

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Background: Herpesviruses are widespread viruses, causing severe infections in both humans and animals. Eradication of herpesviruses is extremely difficult because of their ability to establish latent and lifelong infections. However, latency is only one tool that has evolved in herpesviruses to successfully infect their hosts; such viruses display a wide (and still incompletely known) panoply of genes and proteins that are able to counteract immune responses of their hosts. Envelope glycoproteins and cytokine inhibitors are two examples of such weapons. All of these factors make it difficult to develop diagnostics and vaccines, unless they are based on molecular techniques. Materials and Methods: Animal herpesviruses, because of their striking similarity to human ones, are suitable models to study the molecular biology of herpesviruses and develop strategies aimed at designing neurotropic live vectors for gene therapy as well as engineered attenuated vaccines. Results: BHV-1 is a neurotropic herpesvirus causing infectious rhinotracheitis (IBR) in cattle. It is a major plague in zootechnics and commercial trade, because of its ability to spread through asymptomatic carrier animals, frozen semen, and embryos. Such portals of infections are also important for human herpesviruses, which mainly cause systemic, eye, and genital tract infections, leading even to the development of cancer. Conclusions: This review covers both the genetics and molecular biology of BHV-1 and its related herpesviruses. Epidemiology and diagnostic approaches to herpesvirus infections are presented. The role of herpesviruses in gene therapy and a broad introduction to classic and engineered vaccines against herpesviruses are also provided.

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A gE deleted infectious bovine rhinotracheitis marker vaccine for use in improved bovine herpesvirus 1 control programs

Cited by 49 publications

References 5 publications

Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil

Construction and characterization of a glycoprotein E deletion mutant of bovine herpesvirus type 1.2 strain isolated in Brazil

The serological BHV1 status of dams determines the precolostral status of their calves

BHV-1: New Molecular Approaches to Control a Common and Widespread Infection

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