A gene expression-based comparison of cell adhesion to extracellular matrix and RGD-terminated monolayers

Abstract

This work uses global gene expression analysis to compare the extent to which model substrates presenting peptide adhesion motifs mimic the use of conventional extracellular matrix protein coated substrates for cell culture. We compared the transcriptional activities of genes in cells that were cultured on matrix-coated substrates with those cultured on self-assembled monolayers presenting either a linear or cyclic RGD peptide. Cells adherent to cyclic RGD were most similar to those cultured on native ECM, while cells cultured on monolayers presenting the linear RGD peptide had transcriptional activities that were more similar to cells cultured on the uncoated substrates. This study suggests that biomaterials presenting the cyclic RGD peptide are substantially better mimics of extracellular matrix than are uncoated materials or materials presenting the common linear RGD peptide.

Multivalency in Heteroternary Complexes on Cucurbit[8]uril‐Functionalized Surfaces: Self‐assembly, Patterning, and Exchange Processes

“…The first type of surface consists of a mixed SAM of thiols on gold (surface type 1, Scheme , top). Type 1 surfaces were fabricated by incubation of gold sensors with a mixture of antifouling oligo(ethylene glycol) alkanethiols, 1 % of which contained a maleimide group . Surfaces of type 2 were fabricated on cyclic olefin polymer (COP) platforms coated with a SAM of poly‐L‐lysine grafted with 20 % oligo(ethylene glycol) and 3 % maleimide‐oligo(ethylene glycol) (PLL‐OEG‐Mal).…”

“…Type 1 surfaces were fabricated by incubation of gold sensors with a mixture of antifouling oligo(ethylene glycol) alkanethiols, 1 % of which contained a maleimide group. [39,40] Surfaces of type 2 were fabricated on cyclic olefin polymer (COP) platforms coated with a SAM of poly-L-lysine grafted with 20 % oligo (ethylene glycol) and 3 % maleimide-oligo(ethylene glycol) (PLL-OEG-Mal). For COP substrates (surface type 2), the surface was exposed to oxygen plasma to assure good surface wettability and electrostatic adhesion of PLL-OEG-Mal to the COP substrates [41] (see Scheme 1 ii).…”

Tissue and organ decellularization in regenerative medicine

“…Peptide motifs from collagen I (GTPGPQGIAGQRGVV), collagen IV (MNYYSNS), fibronectin (PHSRN), laminin (YIGSR) can enhance cell attachment . Arginine‐glycine‐aspartic acid (RGD), a peptide sequence found in fibronectin, vitronectin, laminin, collagen types I and IV, has a high affinity for integrin‐mediated cell attachment and spreading, functionality and tissue development . The RGD sequence co‐immobilized with the SVVYGLR sequence was used to enhance the adhesion of endothelial cells .…”

“…124,125 Arginine-glycineaspartic acid (RGD), a peptide sequence found in fibronectin, vitronectin, laminin, collagen types I and IV, has a high affinity for integrin-mediated cell attachment and spreading, functionality and tissue development. [126][127][128][129][130] The RGD sequence co-immobilized with the SVVYGLR sequence was used to enhance the adhesion of endothelial cells. 123 Three ECMderived peptides, laminin (IKLLI and PDSGR) and cadherin (HAVDI) supported the adhesion and survival of insulincontaining cultures for 5 days.…”

Cucurbit[8]uril ternary complexes for biomolecular assemblies in solution and at interfaces

“…68 Mixed self-assembled monolayers were prepared incubating gold coated surfaces with the symmetric tetra(ethylene glycol)-terminated undecyl disulfide as the backfilling component and with the asymmetric maleimide and tri(ethylene glycol)-terminated undecyl disulfide as the reactive component. 69 A…”