A general liquid chromatography tandem mass spectrometry method for the quantitative determination of diquaternary ammonium gemini surfactant drug delivery agents in mouse keratinocytes’ cellular lysate

Buse, Joshua; Badea, Ildikó; Verrall, R. E.; El‐Aneed, Anas

doi:10.1016/j.chroma.2013.04.031

Cited by 18 publications

(43 citation statements)

References 32 publications

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“…One inherent challenge in the ESI-MS/MS is matrix effect and the inconsistency in ion current response when using ESI source [29]. To account for such variations, the use of internal standard that has similar physicochemical properties to the analyte and elutes at the same time can ensure the validity of the quantitative data [21]. Therefore, we used deuterated metformin that bears 6 deuterium atoms, which will ensure that no cross talk will occur between the analyte transitions and the internal standard.…”

Section: Methods Developmentmentioning

confidence: 99%

“…In addition, additives are usually added to the mobile phase to enhance the LC separation and peak shape so that quantitative data can be obtained. Such additives may in some occasions interfere in MS analysis by competing with the target analytes during ionization resulting in undesired ion suppression [21].…”

Section: Introductionmentioning

confidence: 99%

“…However, Nanita et al quantified six pesticides extracted from agricultural products without the need for any column prior to injecting the sample into the mass spectrometry [25]. While generally speaking, matrix effects are expected to increase once the analytical column is removed, Buse et al [26] showed that the removal of the analytical column was beneficial as tri-ethanol amine (TEA), used in LC-MS/MS as ion pairing reagent [21], was no longer needed. The use of TEA resulted in undesirable ion suppression [21] which was eliminated when the analytical column was removed [26].…”

Section: Introductionmentioning

confidence: 99%

“…While generally speaking, matrix effects are expected to increase once the analytical column is removed, Buse et al [26] showed that the removal of the analytical column was beneficial as tri-ethanol amine (TEA), used in LC-MS/MS as ion pairing reagent [21], was no longer needed. The use of TEA resulted in undesirable ion suppression [21] which was eliminated when the analytical column was removed [26]. However, to achieve quantitative data, method development was needed including changes within the mobile phase.…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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A simple, fast and sensitive quantification method for the drug metformin in dog serum was developed using flow injection analysis (FIA)-tandem mass spectrometry (MS/MS). The method was fully validated according to industry standards. It is the first time that FIA-MS/MS for metformin was developed surpassing all existing methods in terms of time of analysis. The quantification method was dependent on the formation of [M+H] + using electrospray ionization (ESI) and employing multiple reaction monitoring (MRM) using quadrupole-linear ion trap (4000 QTRAP ® ) instrument. A deuterated internal standard (IS) of metformin bearing six deuterium atoms was used to compensate for matrix effects and for variation in ion current within the ESI source. The ion transitions that were monitored were m/z 130.1 m/z 71.0 and m/z 130.1 m/z 60.1 for metformin and m/z 136.0 m/z 77.0 for the internal standard. A linear response (r = 0.9966) was established for a range of concentrations of 5-2340 ng/ml. The inter-and intra-day variations were within the acceptable criteria for all quality control samples. The method was successfully applied for measurement of serum metformin concentration in dogs after intravenous injection.

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Section: Methods Developmentmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Michel

Gaunt

Arnason

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

Self Cite

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“…For example, nano-ESI (40) is one approach in which flow rates are substantially reduced, allowing fewer analyte and nonvolatile compounds to be injected into the source while enhancing the desolvation process of the droplets (36,37). Another approach that employs a suitable internal standard (IS) is used, in particular, an isotopically labeled IS that exhibits similar structural and physical properties to the analyte to compensate the matrix effect rather than eliminating it (36,37,41). The latter approach is probably the most widely applied strategy because it addresses not only the variations in ion currents from run to run commonly observed with an ESI source but also the variations in the pre-MS experimental work such as extraction (36).…”

Section: Electrospray Ionizationmentioning

confidence: 99%

Mass Spectrometry, Review of the Basics: Ionization

Awad

Khamis

El‐Aneed

2014

Applied Spectroscopy Reviews

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126

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Mass spectrometry (MS) has become an integral tool in life sciences. The first step in MS analysis is ion formation (ionization). Many ionization methods currently exist; electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) are the most commonly used. ESI relies on the formation of charged droplets releasing ions from the surface (ion evaporation model) or via complete solvent evaporation (charge residual model). MALDI ionization, however, is facilitated via laser energy and the use of a matrix. Despite wide use, ESI cannot efficiently ionize nonpolar compounds. Atmospheric pressure chemical ionization (APCI) and atmospheric pressure photo ionization (APPI) are better suited for such tasks. APPI requires photon energy and a dopant, whereas APCI is similar to chemical ionization. In 2004, ambient MS was introduced in which ionization occurs at the sample in its native form. Desorption electrospray ionization (DESI) and direct analysis in real time (DART) are the most widely used methods. In this mini-review, we provide an overview of the main ionization methods and the mechanisms of ion formation. This article is educational and intended for students/researchers who are not very familiar with MS and would like to learn the basics; it is not for MS experts.

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The development and assessment of high-throughput mass spectrometry-based methods for the quantification of a nanoparticle drug delivery agent in cellular lysate

et al. 2014

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The safe use of lipid-based drug delivery agents requires fast and sensitive qualitative and quantitative assessment of their cellular interactions. Many mass spectrometry (MS) based analytical platforms can achieve such task with varying capabilities. Therefore, four novel high-throughput MS-based quantitative methods were evaluated for the analysis of a small organic gene delivery agent: N,N-bis(dimethylhexadecyl)-1,3-propane-diammonium dibromide (G16-3). Analysis utilized MS instruments that detect analytes using low-resolution tandem MS (MS/MS) analysis (i.e. QTRAP or linear ion trap in this work) or high-resolution MS analysis (i.e. time of flight (ToF) or Orbitrap). Our results indicate that the validated fast chromatography (FC)-QTRAP-MS/MS, FC- LTQ-Orbitrap-MS, desorption electrospray ionization-collision-induced dissociation (CID)-MS/MS and matrix assisted laser desorption ionization-ToF/ToF-MS MS methods were superior in the area of method development and sample analysis time to a previously developed liquid chromatography (LC)-CID-MS/MS. To our knowledge, this is the first evaluation of the abilities of five MS-based quantitative methods that target a single pharmaceutical analyte. Our findings indicate that, in comparison to conventional LC-CID-MS/MS, the new MS-based methods resulted in a (1) substantial reduction in the analysis time, (2) reduction in the time required for method development and (3) production of either superior or comparable quantitative data. The four new high-throughput MS methods, therefore, were faster, more efficient and less expensive than a conventional LC-CID-MS/MS for the quantification of the G16-3 analyte within tissue culture. When applied to cellular lysate, no significant change in the concentration of G16-3 gemini surfactant within PAM212 cells was observed between 5 and 53 h, suggesting the absence of any metabolism/excretion from PAM212 cells.

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A general liquid chromatography tandem mass spectrometry method for the quantitative determination of diquaternary ammonium gemini surfactant drug delivery agents in mouse keratinocytes’ cellular lysate

Cited by 18 publications

References 32 publications

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Development and validation of fast and simple flow injection analysis–tandem mass spectrometry (FIA–MS/MS) for the determination of metformin in dog serum

Mass Spectrometry, Review of the Basics: Ionization

The development and assessment of high-throughput mass spectrometry-based methods for the quantification of a nanoparticle drug delivery agent in cellular lysate

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