2024
DOI: 10.1101/2024.04.28.590365
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A genetic and microscopy toolkit for live imaging of whole-body regeneration inMacrostomum lignano

R. Nelson Hall,
Hongquan Li,
Chew Chai
et al.

Abstract: Live imaging of regenerative processes can reveal how animals restore their bodies after injury through a cascade of dynamic cellular events. Here, we present a comprehensive toolkit for live imaging of whole-body regeneration in the flatworm Macrostomum lignano, including a high throughput cloning pipeline, targeted cellular ablation, and advanced microscopy solutions. Using tissue-specific reporter expression, we examine how various structures regenerate. Enabled by a custom, low-cost luminescence/fluorescen… Show more

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