A genetic linkage map for the domesticated silkworm,<i>Bombyx mori</i>, based on restriction fragment length polymorphisms

Shi, Jinrui; Heckel, David G.; Goldsmith, Marian R.

doi:10.1017/s0016672300034467

Cited by 77 publications

(65 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Linkage analysis: Linkage analysis was performed by taking advantage of the lack of crossing over in female Lepidoptera and the method of forbidden recombinants (Shi et al 1995). The inheritance pattern of each gene was matched to a known ''chromosome print'' from brood 33 that had previously been developed using AFLPs, microsatellites, and genes for each of the 21…”

Section: Methodsmentioning

confidence: 99%

Synteny and Chromosome Evolution in the Lepidoptera: Evidence From Mapping in Heliconius melpomene

et al. 2007

View full text Add to dashboard Cite

The extent of conservation of synteny and gene order in the Lepidoptera has been investigated previously only by comparing a small subset of linkage groups between the moth Bombyx mori and the butterfly Heliconius melpomene. Here we report the mapping of 64 additional conserved genes in H. melpomene, which contributed 47 markers to a comparative framework of 72 orthologous loci spanning all 21 H. melpomene chromosomes and 27 of the 28 B. mori chromosomes. Comparison of the maps revealed conserved synteny across all chromosomes for the 72 loci, as well as evidence for six cases of chromosome fusion in the Heliconius lineage that contributed to the derived 21-chromosome karyotype. Comparisons of gene order on these fused chromosomes revealed two instances of colinearity between H. melpomene and B. mori, but also one instance of likely chromosomal rearrangement. B. mori is the first lepidopteran species to have its genome sequenced, and the finding that there is conserved synteny and gene order among Lepidoptera indicates that the genomic tools developed in B. mori will be broadly useful in other species.

show abstract

Section: Methodsmentioning

confidence: 99%

Synteny and Chromosome Evolution in the Lepidoptera: Evidence From Mapping in Heliconius melpomene

et al. 2007

View full text Add to dashboard Cite

show abstract

“…5 and 6) covering 900.2 cM. In 1995, molecular linkage maps were constructed employing random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphic (RFLP) markers (10,11). After a high-density RAPD map with a marker interval of Ϸ2 cM (Ϸ500 kb) was reported (12), an amplified fragment length polymorphism (AFLP) genetic linkage map was constructed with a logarithm of odds score of 3.0 (13), and more recently a RFLP map with nearly 200 ESTs (14).…”

mentioning

confidence: 99%

“…RFLPs are usually codominant, but require relatively large amounts of progeny DNA, and are not suitable for high throughput screening. Because of the disadvantageous characteristics of these markers, the molecular genetic maps contained a large number of linkage groups with only a few assignments integrated into the ELG map (11,16).…”

mentioning

confidence: 99%

Simple sequence repeat-based consensus linkage map of Bombyx mori

Xing

Xub

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

111

119

View full text Add to dashboard Cite

We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. A survey of six representative silkworm strains using 2,500 (CA)nand (CT)n-based SSR markers revealed 17-24% polymorphism, indicating a high degree of homozygosity resulting from a long history of inbreeding. Twenty-nine SSR linkage groups were established in well characterized Dazao and C108 strains based on genotyping of 189 backcross progeny derived from an F1 male mated with a C108 female. The clustering was further focused to 28 groups by genotyping 22 backcross progeny derived from an F1 female mated with a C108 male. This set of SSR linkage groups was further assigned to the 28 chromosomes (established linkage groups) of silkworm aided by visible mutations and cleaved amplified polymorphic sequence markers developed from previously mapped genes, cDNA sequences, and cloned random amplified polymorphic DNAs. By integrating a visible mutation p (plain, larval marking) and 29 well conserved genes of insects onto this SSRbased linkage map, a second generation consensus silkworm genetic map with a range of 7-40 markers per linkage group and a total map length of Ϸ3431.9 cM was constructed and its high efficiency for genotyping and potential application for synteny studies of Lepidoptera and other insects was demonstrated.silkworm ͉ microsatellite

show abstract

“…In addition, several groups have engaged in the construction of molecular linkage maps in the silkworm by using a variety of markers, with the aims of providing a framework for positional cloning of specific genes and mutations, large-scale physical map construction, analysis of quantitative trait loci, and comparative genomics. To date, Ϸ1,500 markers based on random amplified polymorphic DNAs (3,4), restriction fragment length polymorphisms (5,6), amplified fragment length polymorphisms (7), and microsatellites (8) are available for the construction of molecular linkage maps, which now cover all 28 Bombyx chromosomes at an average spacing of 2 cM, which is equivalent to Ϸ500 kb (4). The extensive genetic resources for B. mori make it an ideal reference for the Lepidoptera, where comparative genetics and genomics can work together to elucidate conserved evolutionary pathways and their diversification, identify new genes and gene systems as targets for transgenesis, and provide basic research leading toward new genome-based approaches for the control of pest species (9).…”

mentioning

confidence: 99%