A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers

Casasoli, M.; Mattioni, Claudia; Cherubini, M.; Villani, F.

doi:10.1007/s00122-001-0553-1

Cited by 101 publications

(91 citation statements)

References 55 publications

(69 reference statements)

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“…(Kubisiak et al 1997;Casasoli et al 2001;Sisco et al 2005) and a substantial advance in Fagaceae genomics. The previous Castanea genetic maps, largely composed of anonymous genetic markers (e.g., RAPDs, ISSRs, and AFLPs), were limited in their usefulness for comparative genomics and applications in molecular breeding.…”

Section: Genetic Maps and Comparative Analysismentioning

confidence: 99%

“…Kubisiak et al (1997) proposed a three QTL model that explained about 70 % of the phenotypic variance for resistance to blight. Genetic maps constructed for ecologically diverse parents of the European chestnut, Castanea sativa (Casasoli et al 2001), have been used to identify QTLs for various adaptive traits such as bud flush, growth, and carbon isotope discrimination .…”

Section: Introductionmentioning

confidence: 99%

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A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

Kubisiak

Nelson

Staton

et al. 2012

Tree Genetics & Genomes

126

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The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) throughout its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the introgression of Chinese chestnut blight resistance genes into American chestnut. Two mapping populations were established with three Chinese chestnut parents, 'Mahogany', 'Nanking', and 'Vanuxem', totaling 337 progeny. The transcriptome-based genetic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most comprehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut consensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus persica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. dentata) F 2 mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci.

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Section: Genetic Maps and Comparative Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

Kubisiak

Nelson

Staton

et al. 2012

Tree Genetics & Genomes

126

View full text Add to dashboard Cite

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“…For its advantages of simple procedure, low-cost, good stability and high reproducibility, ISSR markers has been successfully used in genetic mapping (Casaoli et al 2001;Cekic et al 2001;Tanyolac 2003), germplasm identification (Nagaoka and Ogihara 1997;Potter et al 2002) and genetic diversity analysis (Ash et al 2003;Wu et al 2005). Until now, few studies have been carried on the genetic variations of C. tinctorius L. using ISSR markers (Amiri et al 2001;Guo et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

Panahi

Neghab

2013

Physiol Mol Biol Plants

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Safflower (Carthamus tinctorious L.) is valued as a source of high quality vegetable oil. 20 ISSR primers were used to assess the genetic diversity of 18 accessions of safflower collected from different geographical regions of Iran. The ISSR primers combinations revealed 57.6 % polymorphism, among 338 genetic loci amplified from the accessions. The sum of effective number of alleles and observed number of alleles were 29.76 and 36.77, respectively. To understand genetic relationships among these cultivars, Jacquards' similarity coefficient and UPGMA clustering algorithm were applied to the ISSR marker data set. ISSR markers grouped accessions into two main clusters and four sub clusters. Also, the principal coordinate analysis (PCoA) supported the cluster analysis results. The results showed these genotypes have high genetic diversity, and can be used for alternative safflower breeding program.

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“…Because of these reasons, ISSR are being used for population authentication and population molecular ecology studies. [8][9][10][11][12][13][14][15][16] The present study was undertaken with the objective of establishing specific molecular markers using ISSR for authenticating eight wild D. officinale populations. …”

mentioning

confidence: 99%

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

Shen

Ding

Liu

et al. 2006

Biological & Pharmaceutical Bulletin

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(previous name: Dendrobium candidum WALL. ex LINDL. 2)) is an endangered species in China and ranked "the first of the nine Chinese fairy herbs." The stems of D. officinale have been used as a traditional Chinese tonic medicine called Tiepi Fengdou 3) for hundreds of years. It can benefit human health in many aspects, such as nourishing yin and clearing away unhealthy heat, benefiting the stomach, promoting the production of body fluid, resisting cancer and prolonging life 1,3) This treasured herb is in severe shortage, making it expensive; the highest grade costs ¥12000-30000 per kg. 4)In our previous studies, rDNA ITS regions of D. officinale and other four adulterant species were sequenced. A pair of allele-specific diagnostic primers was designed to authenticate D. officinale from the other species based on rDNA ITS sequences of D. officinale and the other 37 species of Dendrobium.5,6) Recently, we have focused on the authentication of D. officinale populations using DNA molecular markers, which will lay the foundation for the selection of high-quality population resources.Intersimple sequence repeats (ISSR) have become a good DNA molecular marker for research on populations of the same species, a technique established based on PCR by Zietkiewicz et al. in 1994. 7) The main advantages of ISSR are: no need for DNA sequence information prior to amplification, low cost, simple operation, high stability, and abundance of genomic information. Because of these reasons, ISSR are being used for population authentication and population molecular ecology studies. [8][9][10][11][12][13][14][15][16] The present study was undertaken with the objective of establishing specific molecular markers using ISSR for authenticating eight wild D. officinale populations. MATERIALS AND METHODS Plant MaterialsAll materials used in this study were collected in China. Voucher samples were identified by Dr. Xiaoyu Ding and preserved by means of tissue culture in the College of Life Sciences, Nanjing Normal University. Wherever possible, three to five individuals were sampled from each population. The details are shown in Table 1.DNA Extraction Complete genomic DNA samples were extracted from fresh leaves using Dneasy Plant Mini Kits (QIAGEN), the concentrations of the DNA samples were measured spectrophotometrically, and each sample was diluted to 20 ng ml Ϫ1 with TE buffer for PCR amplification. Selection of Primers To select suitable primers for the study of populations of D. officinale, 76 ISSR primers, which were all purchased from Sangon Co., Ltd., China, were screened using two DNA samples. From the preliminary screening, 32 primers that could amplify visible bands were selected for further examination. Eventually, 10 ISSR primers that produced clear and reproducible bands were selected for the amplification of all DNA samples (Table 2).ISSR Profiling ISSR amplifications were carried out in a 25 ml volume containing Tris-HCl 10 mM, KCl 10 mM, (NH 4 ) 2 SO 4 8 mM (pH 9.0), MgCl 2 1.2-1.8 mM, Taq DNA polymerase1.5 U, dNTP 80 mM, pri...

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A genetic linkage map of European chestnut (Castanea sativa Mill.) based on RAPD, ISSR and isozyme markers

Cited by 101 publications

References 55 publications

A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)

Genetic characterization of Iranian safflower (Carthamus tinctorius) using inter simple sequence repeats (ISSR) markers

Intersimple Sequence Repeats (ISSR) Molecular Fingerprinting Markers for Authenticating Populations of Dendrobium officinale KIMURA et MIGO

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