A genetic linkage map of the laboratory rat, Rattus norvegicus

Jacob, Howard J.; Brown, Donna M.; Bunker, Ruth K.; Daly, Mark J.; Dzau, Victor J.; A, Goodman; Koike, George; Křen, Vladimı́r; Kurtz, Theodore W.; Lernmark, Åke; Levan, Göran; Mao, Yi pei; Pettersson, Anna; Pravenec, Michal; Simon, Jason S.; Szpirer, Claude; Szpirer, Josiane; Trolliet, Maria R; Winer, Eric S.; Lander, Eric S.

doi:10.1038/ng0195-63

Cited by 433 publications

(216 citation statements)

References 39 publications

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“…Unlike mouse inbred strains, little is known about relationships among rat strains (Festing 1979). The Allele Characterization Project (ACP; lgr.mcw-edu/ research/lgr_acp.html) was initiated to guide the identification of individual strains, ensure purity of strains used for specific research purposes, and provide a means of mapping genes based on linkage (Jacob et al 1995;Brown et al 1998; Steen et al 1999). The ACP genotyped 48 commonly used rat inbred strains by using over 4800 microsatellite markers with known genomic locations.…”

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Phylogenetics of rat inbred strains

et al. 2003

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Phylogenetics of rat inbred strains

et al. 2003

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“…We purchased a set of over 600 microsatellite markers [2,4] (Research Genetics, Inc., Huntsville, AL), and chose a set of 188 markers which were polymorphic in OLETF and F344 rats. We genotyped 98 (OLETF female × F344 male) F 2 progeny and the parental strains by PCR amplification of the polymorphic markers.…”

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confidence: 99%

“…Furthermore, the human chromosomal region homologous to the rat genomic interval for the chromosome 20 QTL is 6p21 [1,4,14]. This comparative map suggests the glucagon-like peptide-1 receptor (GLP-1R) locus [10] as positional candidate gene, which is physiologically involved in β-cell function.…”

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Identification of a Quantitative Trait Locus Influencing Plasma Insulin Levels after 70% Pancreatectomy Using the OLETF Rat.

Moralejo

Ogino

Zhu

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. The Otsuka Long-Evans Tokushima Fatty (OLETF) rat is an animal model for obese-type non-insulin-dependent diabetes mellitus (NIDDM) in humans. The OLETF rat has poor capacity for proliferation of pancreatic β-cells after partial pancreatectomy, which may be the critical pathogenetic event in NIDDM development. The poor pancreatic β-cell proliferation in this model is characterized by reduction in β-cell mass and decrease in insulin content in the remnant pancreas. Our investigation was designed to identify quantitative trait loci (QTLs) responsible for β-cell mass and plasma insulin levels after partial pancreatectomy by performing a genome-wide scan in an F 2 intercross obtained by mating the OLETF and the Fischer-344 (F344) rats. We have identified a suggestive QTL for the plasma insulin levels, near D20Mgh5 on rat chromosome 20, with a maximum lod score of 3.75 which accounts for 20% of the total variance, while no QTLs were detected for β-cell mass. This chromosome 20 QTL, whose OLETF allele is associated with low plasma insulin levels through acting in an incompletely recessive manner, may affect insulin secretion itself rather than β-cell proliferation. -KEY WORDS: NIDDM, OLETF rat, QTL.J. Vet. Med. Sci. 60(10): 1157-1160, 1998 Hirashima and Kawano, and inbred Fischer-344 (F344) rats were obtained from Charles River Japan, Inc. (Yokohama, Japan). We arranged crosses between female OLETF and male F344 rats to produce 98 male F 2 progeny. Only male rats at 6 weeks of age at the beginning of the study were subjected to 70% pancreatectomy for genetic studies. After an overnight fast, animals were anesthetized with ether and given additional ether if needed during surgery. All pancreatic tissue was removed by gentle abrasion with cotton applicators, except for an anatomically well-defined remnant bordered by the branch of the hepatic portal vein and the first portion of the duodenal loop. After surgery, the rats had food and water available ad libitum. Nonfasting plasma insulin concentrations were measured at 28 days after surgery. Blood samples were obtained by tail snipping, and non-fasting insulin concentrations in the plasma were determined by a commercially available kit (Eiken Kagaku, Tokyo, Japan) based on radioimmunoassay with rat insulin (Novo, Bagsvared, Denmark) as a standard. Pancreas was excised and cleared of extraneous lymph nodes and fat at 28 days after surgery. The pancreas was weighed and cassetted with the same anatomic orientation, placed in Bouin's fixative, and processed for paraffin embedding using a standard protocol. For the measurement of β-cell mass, two sets of three serial sections (3-5 µm thick) were obtained at intervals of ~250 mm. The sections were first deparaffined and then immunostained for insulin by using ABC kit (Amersham, UK). The primary antibody used was polyclonal guinea pig anti-porcine insulin antibody (1:400, Dako Carpinteria). Using Weibel's point-counting morphometrics [13], the relative volume of β-cells was quantitated at 200 × magnification using a 9...

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“…Ten loci were amplified in R. rattus (D11Mgh5, D16Rat81, D5Rat83, D9Rat13, D7Rat13, D10Rat20, RfgC2, RfgD6, RfgL1, RfgL4) using published primers (Jacob et al. 1995; Hinten et al. 1999).…”

Section: Methodsmentioning

confidence: 99%

Cryptic diversity in Black rats Rattus rattus of the Galápagos Islands, Ecuador

Willows‐Munro

Dowler

Jarcho

et al. 2016

Ecology and Evolution

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Human activity has facilitated the introduction of a number of alien mammal species to the Galápagos Archipelago. Understanding the phylogeographic history and population genetics of invasive species on the Archipelago is an important step in predicting future spread and designing effective management strategies. In this study, we describe the invasion pathway of Rattus rattus across the Galápagos using microsatellite data, coupled with historical knowledge. Microsatellite genotypes were generated for 581 R. rattus sampled from 15 islands in the archipelago. The genetic data suggest that there are at least three genetic lineages of R. rattus present on the Galápagos Islands. The spatial distributions of these lineages correspond to the main centers of human settlement in the archipelago. There was limited admixture among these three lineages, and these finding coupled with low rates of gene flow among island populations suggests that interisland movement of R. rattus is rare. The low migration among islands recorded for the species will have a positive impact on future eradication efforts.

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A genetic linkage map of the laboratory rat, Rattus norvegicus

Cited by 433 publications

References 39 publications

Phylogenetics of rat inbred strains

Phylogenetics of rat inbred strains

Identification of a Quantitative Trait Locus Influencing Plasma Insulin Levels after 70% Pancreatectomy Using the OLETF Rat.

Cryptic diversity in Black rats Rattus rattus of the Galápagos Islands, Ecuador

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