A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers

Abstract:The spontaneous emergence among common pea (Pisum sativum L.) cultivars of off-type rogue plants exhibiting leaves with narrower and pointed leaflets and stipules and the non-Mendelian inheritance of this new phenotype were first described in the early 20th century. However, so far, no studies at the molecular level of this first identified case of paramutation have been carried out. In this study, we show for the first time that the pea rogue paramutation is accompanied by alterations in the methylation status of specific genomic sequences. Although, no significant differences were observed in the genome-wide DNA methylation in leaves of non-rogue cv. Onward in comparison to its rogue paramutant line JI2723, 22 DNA sequences were identified by methylation-sensitive amplified fragment length polymorphisms (MS-AFLP) analysis as differentially methylated in the two epigenomes. Mitotically inherited through all leaf tissues, the differential methylation patterns were also found to be meiotically inherited and conserved in pollen grains for 12 out of the 22 sequences. Fourteen of the sequences were successfully amplified in cDNA but none of them exhibited significant differential expression in the two contrasting epigenotypes. The further exploitation of the present research results on the way towards the elucidation of the molecular mechanisms behind this interesting epigenetic phenomenon is discussed.

show abstract

“…PCR amplifications and agarose and polyacrylamide gel electrophoresis were carried out as previously described [28,29].…”

Section: Rapd and Ssr Analysesmentioning

confidence: 99%

The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences

2017

View full text Add to dashboard Cite

show abstract

“…This fruit is cultivated in most tropical and subtropical countries (Carlier, Sousa, Santo, d'Eeckenbrugge & Leitao, 2010). Ranking third among tropical fruits in world production, Ananas comosus is the only member of the family Bromeliaceae that bears edible fruits (Kudom & Kwapong, 2010).…”

Section: Pineapplementioning

confidence: 99%

A Comparative Study of the Antioxidant Properties of Three Pineapple (Ananas comosus L.) Varieties

Yuris

Siow

2014

Journal of Food Studies

View full text Add to dashboard Cite

The objective of this study was to determine the antioxidant activity of different pineapple varieties namely Josephine (Ananas comosus L. var. comosus cv. Josephine), Morris (Ananas comosus L. var. comosus cv. Morris), and Sarawak (Ananas comosus L. var. comosus cv. Sarawak). Different concentrations of methanol, ethanol, and acetone were used to extract the phenolic compounds. Folin Ciocalteu's reagent was used to determine the total phenolic content. The ascorbic acid content of the fruit extracts was determined using the iodine titration method. The antioxidant activity of the pineapples was measured using the 2,2-diphenyl-1-picryhydrzyl (DPPH) radical scavenging assay, the Ferric Reducing Power (FRP) assay and the Ferrous Ion Chelating (FIC) assay. 50% methanol was chosen as the best solvent for extracting the phenolic compounds of the pineapples. Morris pineapple was found to contain the highest TPC (53.8±0.7 mg GAE/100g fruits) and antioxidant activity. This variety was also found to have the highest DPPH scavenging activity (25.2±0.5 mg AA/100g fruits), FRP (29.6 mg GAE/100g fruits) and FIC ability (8%/100g fruits). Despite having a higher TPC than the Sarawak variety, Josephine exhibited a low scavenging activity. Strong positive correlations (R 2 = 0.9943) were observed between the ascorbic acid content and

show abstract

“…A total of 11 (Acom series) and 5 (EST-SSR series) polymorphic SSR markers were derived from Wöhrmann and Weising (2011) and Carlier et al (2012), respectively, in order to evaluate the genetic relationships of the 27 pineapple cultivars and lines in Taiwan. The designed forward primers for the 16 SSR markers were elongated from the M13 (-21) 18-bp sequence (5'-TGTAAAACGACGGCCAGT-3') by fluorescent labeling (Schuelke, 2000).…”

Section: Dna Extraction and Pcr Amplificationmentioning

confidence: 99%

Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers

Lin¹,

Kuan²,

Weng³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The genetic relationships among 27 pineapple [Ananas comosus (L.) Merr.] cultivars and lines were examined using 16 simple sequence repeat (SSR) markers. The number of alleles per locus of the SSR markers ranged from 2 to 6 (average 3.19), for a total of 51 alleles. Similarity coefficients were calculated on the basis of 51 amplified bands. A dendrogram was created according to the 16 SSR markers by the unweighted pair-group method. The banding patterns obtained from the SSR primers allowed most of the cultivars and lines to be distinguished, with the exception of vegetative clones. According to the dendrogram, the 27 pineapple cultivars and lines were clustered into three main clusters and four individual clusters. As expected, the dendrogram showed that derived cultivars and lines are closely related to their parental cultivars; the genetic relationships between pineapple cultivars agree with the genealogy of (2015) their breeding history. In addition, the analysis showed that there is no obvious correlation between SSR markers and morphological characters.In conclusion, SSR analysis is an efficient method for pineapple cultivar identification and can offer valuable informative characters to identify pineapple cultivars in Taiwan.

show abstract

A genetic map of pineapple (Ananas comosus (L.) Merr.) including SCAR, CAPS, SSR and EST-SSR markers

Cited by 27 publications

References 38 publications

The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences

The Pea (Pisum sativum L.) Rogue Paramutation is Accompanied by Alterations in the Methylation Pattern of Specific Genomic Sequences

A Comparative Study of the Antioxidant Properties of Three Pineapple (Ananas comosus L.) Varieties

Cultivar identification and genetic relationship of pineapple (Ananas comosus) cultivars using SSR markers

Contact Info

Product

Resources

About