A genetically related response to iron deficiency stress in muskmelon

Jolley, D. Von; Brown, J. C.; Nugent, Perry E.

doi:10.1007/978-94-011-3294-7_14

Cited by 10 publications

(12 citation statements)

References 7 publications

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“…Hurley et al (1986) proposed that different species may exhibit the Fe-stress response to varying degrees and this has been shown to be true in several species (Marschner et al, 1986). Jolley et al (1991) showed that muskmelon (Cucumis meló L.) did not exhibit reductant release typical to most dicotyledonous species and that H + release was high compared to other species. Thus, species and even cultivars within species differ in the magnitude of exhibition of the various parts of the Fe-deficiency stress response as well as which individual response in each cultivar or species dominates.…”

Section: Resultsmentioning

confidence: 96%

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Use of hydrogen release or a combination of hydrogen release and iron reduction for selecting iron‐efficient dry bean and soybean cultivars

Ellsworth

Jolley

Nuland

et al. 1998

Journal of Plant Nutrition

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1998) Use of hydrogen release or a combination of hydrogen release and iron reduction for selecting iron-efficient dry bean and soybean cultivarsABSTRACT Iron (Fe) chlorosis continues to be a problem in the world where crops are grown in calcareous soils. Although it is possible to remedy this problem with fertilizer application, selection of cultivars resistant to Fe-deficiency chlorosis is a more economical and practical solution. In addition to field chlorosis rating studies, several methods have been developed to screen cultivars. One of the most promising is quantifying individual plant response mechanisms induced during Fe-deficiency stress, such as Fe reduction or hydrogen ion (H+) extrusion by the roots. Eight dry bean (Phaseolus vulgaris L.) and 11 soybean (Glycine max L.) cultivars of varying resistance to Fe-deficiency chlorosis were grown in solutions of low (0.05 mg L -1 ) and no (0 mg L -1 ) Fe, 2639 ELLSWORTH ET AL.respectively. Beginning at day 2 after imposition of low Fe treatments, plant roots were incubated for one hour in a solution to collect H + ions. This solution was then titrated with NaOH to the pH of the control solutions (no plants grown in solution, but air bubbled through it for one hr). The results of six consecutive days were summed and correlated with field chlorosis scores. In dry bean, there was a statistically significant positive relationship between H + release and field chlorosis scores, which is opposite of the theoretical relationship, i.e., dry bean cultivars with high chlorosis scores released the largest quantity of H + ion and vice versa. Soybean exhibited a statistically significant negative relationship between quantity of H + release and field chlorosis scores, but Fe reduction measurements provided better screening ability than H + ion release. Adjusting the measurements for fresh root weight did not alter these relationships; thus, eliminating the need to weigh roots in a breeding nursery. Combining the effects of H + release and Fe reduction using standardized scores did not improve the selection of Fe efficient cultivars over Fe reduction alone in either dry bean or soybean. Hydrogen ion quantification was time consuming, tedious, and gave conflicting results in the two species studied. Thus, it is not recommended as a screening technique in either dry bean or soybean.

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Section: Resultsmentioning

confidence: 96%

“…Hurley et al (1986) suggested that each species may respond to Fe deficiency in varying ways and degrees. Jolley and Brown (1987) and Jolley et al (1991 ) also found that species vary in the level of activity of the several mechanisms induced by Fe-deficiency stress.…”

Section: Introductionmentioning

confidence: 91%

Use of hydrogen release or a combination of hydrogen release and iron reduction for selecting iron‐efficient dry bean and soybean cultivars

Ellsworth

Jolley

Nuland

et al. 1998

Journal of Plant Nutrition

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“…In the case of H. niger root cultures, the minimum pH was between 4.4~4.6 [36]. In sugar beet [34], pH reached approximately 3.7 after 15days, and pH was maintained at 3.5~4.0 in muskmelon [10]. In H. albus hairy roots, the minimum pH was around 4.0 when a single root tip was subjected to iron deficiency and around 4.5 when propagated roots did.…”

Section: Discussionmentioning

confidence: 98%

Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency

Higa

Miyamoto

Rahman

et al. 2008

Plant Physiology and Biochemistry

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SummaryHyoscyamus albus hairy roots with/without an exogenous gene (11 clones) were established by inoculation of Agrobacterium rhizogenes. All clones cultured under iron deficient condition secreted riboflavin from root tips into the culture medium and the productivity depended on the number and size of root tips among the clones, although the addition of sucrose was essential for riboflavin production. A decline of pH was observed before riboflavin production and root development using either a root tip or propagated roots: propagated roots were employed for further work due to their lesser variation.Additions of proton-pump inhibitors, N,N'-dicyclohexylcarbodiimide (DCCD) at 100 and 10 μM and erythrosine B at 100 μM, suppressed the pH decline at 100 and 10 μM accompanied by inhibition of riboflavin secretion and root growth; at 10 μM of erythrosine B, pH decline occurred with a moderate delay, but both growth and riboflavin efflux were inhibited. Neither inhibition of the pH decline nor riboflavin production was observed at 1 μM. To examine the necessity of acidification and riboflavin secretion by the roots themselves, artificial pH reduction of culture medium with organic acids and the addition of exogenous riboflavin with/without pH reduction were performed. When hairy roots were cultured in iron-deficient medium acidified with citric acid (pH 4.0) or malic acid (pH 3.7), pH increased rapidly to around 5 overnight, following which riboflavin production and root growth occurred. Addition of riboflavin did not affect riboflavin secretion by the roots, but acidification with citric acid (pH 4.0) helped achiever greater riboflavin production and earlier pH elevation. These results indicate that riboflavin efflux does not directly connected to active pH reduction, and more significantly active riboflavin secretion occurs by internal requirement in H. albus hairy roots under iron deficiency.

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“…Thus, differences in phytosiderophore-mediated Fe uptake among the Fe-inefficient muskmelon, tomato, and soybean cannot be explained by currently understood Fe-acquisition mechanisms because: a) nutrient solution pH was buffered at 6.5 to 7.5 and none of these cultivars possess the Fe-stress response mechanism of enhanced H+ release, b) none of these cultivars exude reductants to combat Fe-deficiency stress, and c) reported Fe 3+ reduction at the root plasmalemma when chelated Fe is present is similarly low for fefe muskmelon,T3238fer tomato and T203 soybean (3,14).…”

Section: Phytosiderophore-mediated Iron Uptake In Dicotsmentioning

confidence: 93%

Plant utilization of iron solubilized by oat phytosiderophore

Hopkins¹,

Jolley²,

Brown³

1992

Journal of Plant Nutrition

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Responses to Fe-deficiency stress have been classified as Strategies I and II. "Iron-efficient" cultivare within each strategy exhibit one or more of a combination of specific physiological responses to Fe-deficiency stress, while "Feinefficient" cultivars do not. Iron-efficient Coker 227 oat (Avena byzantina C. Koch.) is a Strategy II plant which produces an Fe-chelating compound (phytosiderophore) in response to Fe-deficiency stress. The objective of this paper is to summarize the results of studies in which the abilities of several plant species to utilize Fe solubilized by oat phytosiderophore were determined. Experiments were conducted in which Fe-deficiency stressed oat plants were grown in hydroponic solutions (1.2 mg Fe L -1 unchelated) in combination with three different species of both monocotyledonous and dicotyledonous plants which represented a wide range of Fe-efficiency. The cultivars varied in response to the oat phytosiderophore as follows: (a) WF9 maize (Zea mays L.), TAM 0-312 oat, 'Redlan' B-line sorghum (Sorghum bicolor (L.) Moench), fefe muskmelon (Cucumis melo L.), and T3238fer tomato (Lycopersicon esculentum Mill.) plants obtained more Fe when grown in combination with Coker 227 oat than when grown alone, (b) ys 1 (ys 1 /ys 1 ) maize was unable to obtain Fe whether grown in the presence or absence of oat, and (c) A7, Dawson, Corsoy 79, Pride B-216, and T203 (PI-54619-5-1) soybean (Glycine max (L.) Merr.) did not obtain more Fe when grown in combination with oat-instead phytosiderophore prevented Fe 1599

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A genetically related response to iron deficiency stress in muskmelon

Cited by 10 publications

References 7 publications

Use of hydrogen release or a combination of hydrogen release and iron reduction for selecting iron‐efficient dry bean and soybean cultivars

Use of hydrogen release or a combination of hydrogen release and iron reduction for selecting iron‐efficient dry bean and soybean cultivars

Root tip-dependent, active riboflavin secretion by Hyoscyamus albus hairy roots under iron deficiency

Plant utilization of iron solubilized by oat phytosiderophore

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