A genome sequence survey of the filarial nematode Brugia malayi: repeats, gene discovery, and comparative genomics

Whitton, Claire; Daub, Jennifer; Quail, Mike; Hall, Neil; Foster, Jeremy M.; Ware, Jennifer; Ganatra, Mehul B.; Slatko, Barton E.; Barrell, Bart; Blaxter, Mark

doi:10.1016/j.molbiopara.2004.05.013

Cited by 28 publications

(22 citation statements)

References 61 publications

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“…Congruent with intra-chromosomal rearrangement far exceeding interchromosomal translocations, analysis of conserved synteny between B. malayi and C. elegans revealed a preponderance of within-chromosome linkage, but little local synteny (homologs of genes separated w60 kb in B. malayi were separated by a mean of O4 Mb in C. elegans) [28]. B. malayi has five chromosome pairs (Table 1) [28], and comparisons of genes linked in B. malayi but on different chromosomes in C. elegans suggests that the changed chromosome number is the result of a fusion between genomic segments corresponding to C. elegans chromosomes I and III.…”

Section: Box 2 Comparing Full and Partial Genomesmentioning

confidence: 99%

“…B. malayi has five chromosome pairs (Table 1) [28], and comparisons of genes linked in B. malayi but on different chromosomes in C. elegans suggests that the changed chromosome number is the result of a fusion between genomic segments corresponding to C. elegans chromosomes I and III. Because the related filarioid Setaria is reported as having nZ6 [20], the fusion could have occurred in the onchocercine lineage (Clade III).…”

Section: Box 2 Comparing Full and Partial Genomesmentioning

confidence: 99%

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Comparative genomics of nematodes

et al. 2005

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Section: Box 2 Comparing Full and Partial Genomesmentioning

confidence: 99%

Section: Box 2 Comparing Full and Partial Genomesmentioning

confidence: 99%

Comparative genomics of nematodes

et al. 2005

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“…Indeed, 70% (1254/1793) of the D. immitis clusters showed similarities to the B. malayi draft genome assembly (October 2005). The actual number of matches to the genome is likely higher because the current B. malayi assembly contains gaps and has only 11,749 annotated gene models (E. Ghedin, personal communications), while about 18,500 genes have been proposed previously according to a genome sequence survey (Whitton et al, 2004). Furthermore, the majority of the similarities across the two filarial species are much stronger than those detected towards genes from nematodes of other clades (e.g.…”

Section: Similarities To Genes From Other Speciesmentioning

confidence: 99%

Identification and analysis of genes expressed in the adult filarial parasitic nematode Dirofilaria immitis

Yin

Martin

McCarter

et al. 2006

International Journal for Parasitology

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The heartworm Dirofilaria immitis is a filarial parasitic nematode infecting dogs and other mammals worldwide causing fatal complications. Here, we present the first large-scale survey of the adult heartworm transcriptome by generation and analysis of 4005 expressed sequence tags, identifying about 1800 genes and expanding the available sequence information for the parasite significantly. Brugia malayi genomic data offered the most valuable information to interpret heartworm genes, with about 70% of D. immitis genes showing significant similarities to the assembly. Comparative genomic analyses revealed both genes common to metazoans or nematodes and genes specific to filarial parasites that may relate to parasitism. Characterization of abundant transcripts suggested important roles for genes involved in energy generation and antioxidant defense in adults. In particular, we proposed that adult heartworm likely adopted an anaerobic electron transfer-based energy generation system distinct from the aerobic pathway utilized by its mammalian host, making it a promising target in developing next generation macrofilaricides and other treatments. Our survey provided novel insights into the D. immitis transcriptome and laid a foundation for further comparative studies on biology, parasitism and evolution within the phylum Nematoda. q

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“…Surprisingly, a comparison of B. malayi BAC sequences with the C. elegans and C. briggsae genomes identified 2789 new B. malayi protein-coding genes, not present in either C. elegans or C. briggsae, highlighting the diversity within the nematodes (Whitton et al 2004). The 26,000 B. malayi ESTs have been clustered into about 8,000 genes.…”

Section: Introductionmentioning

confidence: 99%

Genes encoding putative biogenic amine receptors in the parasitic nematode Brugia malayi

et al. 2007

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Filarial nematodes, such as Brugia malayi, cause major health problems worldwide. The lack of a vaccine against B. malayi, combined with ineffective chemotherapy against the adult has prompted the examination of biogenic amine receptors (BARs) as possible targets for drug discovery. We employed bioinformatics to identify genes encoding putative B. malayi BARs. Surprisingly, the B. malayi genome contains half of the genes predicted to encode BARs in the genomes of free-living nematodes such as Caenorhabditis elegans or C. briggsae; however, all of the predicted B. malayi receptors have clear orthologues in C. elegans. The B. malayi genes encode each of the major BAR subclasses, including three serotonin, two dopamine and two tyramine/octopamine receptors and the structure of orthologous BAR genes is conserved. We find that potential G-protein coupling and ligand-specificity of individual BARs may be predicted by phylogenetic comparisons. Our results provide a framework for how G-protein coupled receptors may be targeted for drug development in medically important parasitic nematodes.

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A genome sequence survey of the filarial nematode Brugia malayi: repeats, gene discovery, and comparative genomics

Cited by 28 publications

References 61 publications

Comparative genomics of nematodes

Comparative genomics of nematodes

Identification and analysis of genes expressed in the adult filarial parasitic nematode Dirofilaria immitis

Genes encoding putative biogenic amine receptors in the parasitic nematode Brugia malayi

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