A genome-wide association and gene–environment interaction study for serum triglycerides levels in a healthy Chinese male population

Tan, Aihua; Sun, Jielin; Xia, Ning; Qin, Xue; Hu, Yanling; Zhang, Shijun; Tao, Sha; Gao, Yong; Yang, Xiaobo; Zhang, Haiying; Kim, Seong-Tae; Peng, Tao; Lin, Xiaoling; Li, Li; Mo, Linjian; Liang, Zhengjia; Shi, Deyi; Zhang, Huang; Huang, Xianghua; Liu, Ming; Ding, Qiang; Trent, Jeffrey M.; Zheng, S. Lilly; Mo, Zengnan; Xu, Jianfeng

doi:10.1093/hmg/ddr587

Cited by 70 publications

(62 citation statements)

References 22 publications

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“…18 www.nature.com/gene between the two stages in age distribution (37.54 versus 37.31 years, P ¼ 0.54), body mass index (23.31 versus 23.46 kg m À 2 , P ¼ 0.18) and smoking behavior (P ¼ 0.66), excepting for alcohol consumption (P ¼ 0.02). According to the results of independent samples t-test (Table 1), we found that there were significant differences on serum IgG level between drinkers and non-drinkers in the first stage (Po0.001), whereas no significant difference was detected in the second stage (P ¼ 0.158).…”

Section: Resultsmentioning

confidence: 99%

“…18 Briefly, about 10 ml overnight fasting venous blood specimens were collected between 0800 and 1100 h in the morning and were transported frozen to the testing center of Department of Clinical Laboratory at the First Affiliated Hospital of Guangxi Medical University in Nanning in two hours, which were centrifuged within 15 to 25 min and stored at À 80 until analysis. IgG was measured with electrochemiluminescence immunoassay on COBAS 6000 system E601 (Elecsys module) immunoassay analyzer (Roche Diagnostics, GmbH, Mannheim, Germany) with the same batch of reagents, and the inter-assay coefficient of variation was 4.97%.…”

Section: Measurement Of Iggmentioning

confidence: 99%

“…The design of this two-stage GWAS had been described in detail elsewhere. 18 Both smoking and drinking in two stages were assessed on the basis of a selfadministered life-style questionnaire according to the same protocol. Respondents that reported smoking currently (daily smoking 46 moths) were coded as smokers, and those reported drinking any beverage 'more than once a year' were coded as drinkers, whereas others were nondrinkers.…”

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confidence: 99%

“…Respondents that reported smoking currently (daily smoking 46 moths) were coded as smokers, and those reported drinking any beverage 'more than once a year' were coded as drinkers, whereas others were nondrinkers. 18,35 Our study research protocol was approved by the local Ethics Committee and Informed consent of each participant was obtained with written documentation. …”

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confidence: 99%

“…18,35 Our study research protocol was approved by the local Ethics Committee and Informed consent of each participant was obtained with written documentation.…”

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Genome-wide association study identifies common variants at TNFRSF13B associated with IgG level in a healthy Chinese male population

Liao

Zhang

et al. 2012

Genes Immun

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IgG has a crucial role in humoral immune response. Serum IgG level is mainly determined under genetic control. To explore the genetic influence on serum IgG levels, a two-stage genome-wide association study (GWAS) was performed in a healthy Chinese population of 3495 men, including 1999 unrelated subjects in the first stage and 1496 independent individuals in the second stage. Three single-nucleotide polymorphisms (SNPs) located in TNFRSF13B on 17p11.2 or nearby were significantly associated with IgG level: rs4792800 in the intron (combined P-value ¼ 1.45 Â 10 À 12 ), rs12603708 in the intron (combined P-value ¼ 1.82 Â 10 À 8 ) and rs3751987 at B65 kb downstream of the 5 0 -UTR region of TNFRSF13B (combined P-value ¼ 3.67 Â 10 À 9 ). Additionally, smoking was identified to be associated with IgG level in both stages (Po0.001), but there was no significant interaction between smoking and the identified SNPs (P40.05). The strong association between variants at TNFRSF13B and IgG level may be helpful to further explore the biological mechanism by which the serum IgG is affected by transmembrane activator, calcium modulator and cyclophilin ligand interactor encoded by TNFRSF13B.Genes and Immunity (2012) 13, 509-513; doi:10.1038/gene.2012.26; published online 7 June 2012Keywords: IgG; genome-wide association studies; TNFRSF13B; TACI; smoking INTRODUCTION IgG, constituting some 80% of total serum immunoglobulin, is the most abundant class of antibodies (Ab) in serum and extracellular fluid. It has an important role in the defense against various pathogens, toxins and even cancers. 1 As the only isotype that can pass through the human placenta, IgG is able to protect the fetus in utero. 2 Meanwhile, IgG gets involved in chronic inflammatory processes contributed to the destruction of healthy tissues in autoimmune diseases, such as arthritis and systemic lupus erythematosus. 3 Serum IgG level varies considerably from one person to another, 4 but little when testing the same person repeatedly. 5 This variation in the serum IgG concentration reflects the balance among the rates of synthesis and catabolism. 6 Multitudinous environmental factors including infections, smoking and chemical compounds can contribute to this variation. 7-9 Reports of pedigree studies or twin studies 10,11 have shown that genetic factors are more important than environmental exposures in determining serum immunoglobulin level in human. 12,13 The values of genetic heritability was h 2 ¼ 0.617 þ 0.020 for IgG. 6 Besides, several studies in a population of common variable immunodeficiency patients or IgA deficiency patients implied that there might be an association between some gene loci and serum IgG level. 14,15 However, to the best of our knowledge, no study has ever been conducted to identify the immune loci directly involved in the regulation of the IgG level.Genome-wide association study (GWAS) is a powerful and unbiased tool for the identification of common genetic variants associated with complex traits. 16 This hypothesis-free approach base...

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Section: Resultsmentioning

confidence: 99%

Section: Measurement Of Iggmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

“…18,35 Our study research protocol was approved by the local Ethics Committee and Informed consent of each participant was obtained with written documentation.…”

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confidence: 99%

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