A genomics approach towards salt stress tolerance

Bohnert, Hans J.; Ayoubi, Patricia; Borchert, Chris; Bressan, Ray A.; Burnap, Robert L.; Cushman, John C.; Cushman, Mary Ann; Deyholos, Michael K.; Fischer, Reinhard; Galbraith, David W.; Hasegawa, Paul M.; Jenks, M. Harley; Kawasaki, Shinji; Koiwa, Hisashi; Kore-eda, Shin; Lee, Byeong-ha; Michalowski, Christine B.; Misawa, E.A.; Nomura, Masatoshi; Öztürk, Neslihan Z.; Postier, Bradley L.; Prade, Rolf A.; Song, Chun‐Peng; Tanaka, Yukinori; Wang, Hong; Zhu, Jian

doi:10.1016/s0981-9428(00)01237-7

Cited by 176 publications

(89 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Upon this stress, algae exhibit a wide range of adaptations at the molecular, cellular and organism level (Hare & Cress, 1997;Bohnert et al, 2001). To counteract the negative effects of osmotic stress on Correspondence to: Bettina Scholz.…”

Section: Introductionmentioning

confidence: 99%

Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities

Scholz

Liebezeit

2012

European Journal of Phycology

View full text Add to dashboard Cite

Diatoms inhabiting intertidal flats are subject to strongly changing salinities due to exposure to rain and desiccation at low tide. In order to determine the physiological responses of marine benthic diatoms to salinity changes, cultures of Navicula phyllepta, Achnanthes delicatula subsp. hauckiana and Nitzschia constricta, isolated from the Soltho¨rn tidal flat (lower Saxony, southern North Sea), were used to study the effect on growth rates of different salinities (0.5, 10, 20, 30, 40, 50). During short (1, 3, 10, 60 min) and long exposures (30 days), the composition of free amino acids and the accumulation of polyols, saccharides, quaternary ammonium compounds and -dimethylsulphoniopropionate (DMSP) were determined. The growth rates of N. phyllepta were not affected by salinity in the tested range, synthesizing and accumulating proline as the only response to salinity increases in the growth medium. The growth responses of A. delicatula subsp. hauckiana were significantly weaker at the lowest and highest salinities in comparison to the control treatment (at a salinity of 30), producing proline, glycine betaine, glycerol and the quaternary ammonium compound homarine. In contrast, N. constricta showed the highest growth rate in the low-salinity treatment and the lowest proline concentrations in the high-salinity assay in comparison to the other species. In addition, N. constricta was the only species that synthesized the tertiary sulphonium compound DMSP. Furthermore, results from the salinity treatments showed particular similarities in compound accumulation. While quaternary ammonium compounds as well as DMSP were synthesized during high-salinity treatments, polyols and carbohydrates were found predominantly in the low-salinity experiments. Enzyme activities specific to proline metabolism in A. delicatula and N. constricta indicated that the high salinity-induced free proline accumulation may be due to a stimulation of synthesis via the ornithine pathway, whereas in N. phyllepta the glutamate pathway may contribute significantly to the free proline accumulation. In conclusion, significant differences in osmolyte compositions were found in the three diatom species during exposure to different salinities, suggesting specific intracellular acclimation processes that provide possible explanations of the species' insensitivity towards environmental short-time salinity variations.

show abstract

Section: Introductionmentioning

confidence: 99%

Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities

Scholz

Liebezeit

2012

European Journal of Phycology

View full text Add to dashboard Cite

show abstract

“…A significant number (nearly half) of the salt-stress-regulated genes encode proteins whose roles cannot be predicted from their primary amino acid sequences (Aubourg & Rouze 2001;Bohnert et al . 2001).…”

Section: Introductionmentioning

confidence: 99%

Over‐expression of the water and salt stress‐regulated Asr1 gene confers an increased salt tolerance

Kalifa

Perlson

Gilad

et al. 2004

Plant Cell & Environment

107

View full text Add to dashboard Cite

ASR1 is a plant-specific, highly charged, low molecular weight polypeptide. Purified ASR1 was shown to posses sequence specific Zn 2+ + + + -dependent DNA binding activity (Kalifa et al . Biochemical Journal 381, 373-378, 2004). Steady-state levels of tomato Asr1 mRNA and protein are transiently increased following exposure of plants to polyethylene glycol, NaCl or abscisic acid. The biological role of ASR1 could not be deduced from sequence analyses or sequence homologies. Tobacco plants over-expressing tomato ASR1 have a decreased rate of water loss and improved salt tolerance. Upon exposure to salt, ASR1-over-expressing plants accumulate less Na + + + + and proline than wild-type plants, and also results in increased steadystate levels of other gene products under non-stressed plant growth conditions. Therefore, ASR1 is probably involved in the regulation of water-or salt-stress-modulated gene expression.

show abstract

“…The resulted matches were annotated. Classification of annotated sequences was according to the method described in [33].…”

Section: Sequence Analysismentioning

confidence: 99%

“…Those commonly expressed cDNAs were excluded for analysis. After searching against the GenBank by BlastX, the gene functions of the cDNA fragments were classified according to Bohnert et al [33]. Among them, 28 cDNA clones were preferentially expressed in the wild type flower buds, and 24 expressed in the peloric mutants (Fig.…”

Section: Morphology Of Peloric Mutant Flowersmentioning

confidence: 99%

Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture

Chen

Tsai

et al. 2005

Cell Res

View full text Add to dashboard Cite

A genomics approach towards salt stress tolerance

Cited by 176 publications

References 47 publications

Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities

Compatible solutes in three marine intertidal microphytobenthic Wadden Sea diatoms exposed to different salinities

Over‐expression of the water and salt stress‐regulated Asr1 gene confers an increased salt tolerance

Transcription analysis of peloric mutants of Phalaenopsis orchids derived from tissue culture

Contact Info

Product

Resources

About