A glimpse into the effect of sulfur supply on metabolite profiling, glutathione and phytochelatins in Panicum maximum cv. Massai exposed to cadmium

Rabêlo, Flávio Henrique Silveira; Fernie, Alisdair R.; Navazas, Alejandro; Borgo, Lucélia; Keunen, Els; Alcântara, Berenice Kussumoto de; Cuypers, Ann

doi:10.1016/j.envexpbot.2018.04.003

Cited by 39 publications

(31 citation statements)

References 74 publications

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“…An alternative mechanism to embed heavy metal chelates in plant cells via high-affinity groups is adopted by plants to detoxify heavy metals. Several thiol-containing groups, including NPT, PCs, and cysteine are critical high-affinity groups that play a vital role in the detoxification and homeostasis of heavy metals in plants 36,37 . Particularly PCs, which chelate Cd in the cytosol as a low molecular weight complex that is transported into the vacuole where they ultimately form a more stable storage state of high molecular weight Cd and PC complexes 21 .…”

Section: Discussionmentioning

confidence: 99%

Effects of exogenous sulfur on alleviating cadmium stress in tartary buckwheat

Wang

et al. 2019

Sci Rep

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Supplying exogenous sulfur-rich compounds increases the content of glutathione(GSH) and phytochelatins(PCs) in plant tissues, enabling plants to enhance their cellular defense capacity and/or compartmentalize Cadmium(Cd) into vacuoles. However, the mechanism by which surplus S modulates tolerance to Cd stress in different tissues need further investigation. In the present study, we found that supplementing the tartary buckwheat( Fagopyrum tararicum ) exposed to Cd with surplus S reversed Cd induced adverse effects, and increased Cd concentrations in roots, but decreased in leaves. Further analysis revealed that exogenous S significantly mitigated Cd-induced oxidative stress with the aids of antioxidant enzymes and agents both in leaves and roots, including peroxidase(POD), ascorbate peroxidase(APX), glutathione peroxidase(GPX), glutathione S-transferase(GST), ascorbic acid(AsA), and GSH, but not superoxide dismutase(SOD) and catalase(CAT). The increased Cd uptake in root vacuoles and decreased translocation in leaves of exogenous S treated plants could be ascribed to the increasing Cd binding on cell walls, chelation and vacuolar sequestration with helps of non-protein thiols(NPT), PCs and heavy metal ATPase 3(FtHMA3) in roots, and inhibiting expression of FtHMA2 , a transporter that helps Cd translocation from roots to shoots. Results provide the fundamental information for the application of exogenous S in reversal of heavy metal stress.

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Section: Discussionmentioning

confidence: 99%

Effects of exogenous sulfur on alleviating cadmium stress in tartary buckwheat

Wang

et al. 2019

Sci Rep

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“…peptides involved in Cd chelation and its transport from the cytosol to the vacuole (Seth et al 2012). Phytochelatin‐mediated Cd storage in the vacuole was reported as an efficient defense strategy in non‐hyperaccumulator plants (Jia et al 2020), as also observed by Santos et al (2011) in B. decumbens and by Rabêlo et al (2018c) in P. maximum . Jia et al (2018) described that defense mechanisms involved in Cd tolerance in Pinus sylvestris were less efficient at ambient compared to higher temperature, despite accumulating more Cd in the roots at the elevated temperature.…”

Section: Introductionmentioning

confidence: 53%

“…cv. Massai tolerated very high Cd concentrations in hydroponics (Kopittke et al 2010, Santos et al 2011, Rabêlo et al 2017a, 2017b, 2017c, 2018b, 2018c, 2018d, 2019, 2020). However, the mechanisms that allow them to cope with Cd‐induced stress are still poorly understood, especially under environmentally realistic conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The most important enzymes involved in antioxidative defense are superoxide dismutases (SOD, EC 1.15.1.1) which dismutate superoxide (O 2 •‐ ) into hydrogen peroxide (H 2 O 2 ) and H 2 O; ascorbate peroxidases (APX, EC 1.11.1.11), catalases (CAT, EC 1.11.1.6) and guaiacol peroxidases (GPX, EC 1.11.1.7) that are involved in reducing H 2 O 2 into H 2 O; and glutathione reductases (GR, EC 1.6.4.2) which convert GSSG into GSH (Szopiński et al 2019, Soares et al 2019a). Although antioxidative enzymes act on ROS scavenging, SODs, CATs, APXs and GPXs apparently are not as important as GSH for mitigating the Cd‐induced oxidative stress in P. maximum (Rabêlo et al 2017b, 2018c, 2018d). Therefore, symptoms of oxidative stress in this plant species can be consequence of GSH depletion due to its oxidation, direct Cd binding or its use for synthesis of PCs (Clemens 2006, Jia et al 2020).…”

Section: Introductionmentioning

confidence: 99%

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Unraveling the mechanisms controlling Cd accumulation and Cd‐tolerance in Brachiaria decumbens and Panicum maximum under summer and winter weather conditions

et al. 2020

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We evaluated the mechanisms that control Cd accumulation and distribution, and the mechanisms that protect the photosynthetic apparatus of Brachiaria decumbens Stapf. cv. Basilisk and Panicum maximum Jacq. cv. Massai from Cd‐induced oxidative stress, as well as the effects of simulated summer or winter conditions on these mechanisms. Both grasses were grown in unpolluted and Cd‐polluted Oxisol (0.63 and 3.6 mg Cd kg−1 soil, respectively) at summer and winter conditions. Grasses grown in the Cd‐polluted Oxisol presented higher Cd concentration in their tissues in the winter conditions, but the shoot biomass production of both grasses was not affected by the experimental conditions. Cadmium was more accumulated in the root apoplast than the root symplast, contributing to increase the diameter and cell layers of the cambial region of both grasses. Roots of B. decumbens were more susceptible to disturbed nutrients uptake and nitrogen metabolism than roots of P. maximum. Both grasses translocated high amounts of Cd to their shoots resulting in oxidative stress. Oxidative stress in the leaves of both grasses was higher in summer than winter, but only in P. maximum superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities were increased. However, CO2 assimilation was not affected due to the protection provided by reduced glutathione (GSH) and phytochelatins (PCs) that were more synthesized in shoots than roots. In summary, the root apoplast was not sufficiently effective to prevent Cd translocation from roots to shoot, but GSH and PCs provided good protection for the photosynthetic apparatus of both grasses.

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“…In addition, the overexpression of serine acetyltransferase and cysteine synthase can result in an increased level of cysteine and γ-glutamylcysteine (γ-EC) in tobacco, thereby ultimately leading to the synthesis of PCs to chelate Cd [36]. Research on Massai grass has shown that the synthesis of GSH and PCs is increased under proper S supply, which confers the tolerance of this plant to Cd [37]. Furthermore, after synthesis, (h)PCs can bind Cd to form (h)PC-Cd complexes and then be transported to vacuoles by ATP-binding-cassette (ABC) transporters [20,38].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

et al. 2020

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Background: Hydrogen gas (H 2) is hypothesised to play a role in plants that are coping with stresses by regulating signal transduction and gene expression. Although the beneficial role of H 2 in plant tolerance to cadmium (Cd) has been investigated previously, the corresponding mechanism has not been elucidated. In this report, the transcriptomes of alfalfa seedling roots under Cd and/or hydrogen-rich water (HRW) treatment were first analysed. Then, the sulfur metabolism pathways were focused on and further investigated by pharmacological and genetic approaches. Results: A total of 1968 differentially expressed genes (DEGs) in alfalfa seedling roots under Cd and/or HRW treatment were identified by RNA-Seq. The DEGs were classified into many clusters, including glutathione (GSH) metabolism, oxidative stress, and ATP-binding cassette (ABC) transporters. The results validated by RT-qPCR showed that the levels of relevant genes involved in sulfur metabolism were enhanced by HRW under Cd treatment, especially the genes involved in (homo)glutathione metabolism. Additional experiments carried out with a glutathione synthesis inhibitor and Arabidopsis thaliana cad2-1 mutant plants suggested the prominent role of glutathione in HRW-induced Cd tolerance. These results were in accordance with the effects of HRW on the contents of (homo)glutathione and (homo)phytochelatins and in alleviating oxidative stress under Cd stress. In addition, the HRW-induced alleviation of Cd toxicity might also be caused by a decrease in available Cd in seedling roots, achieved through ABC transportermediated secretion. Conclusions: Taken together, the results of our study indicate that H 2 regulated the expression of genes relevant to sulfur and glutathione metabolism and enhanced glutathione metabolism which resulted in Cd tolerance by activating antioxidation and Cd chelation. These results may help to elucidate the mechanism governing H 2-induced Cd tolerance in alfalfa.

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A glimpse into the effect of sulfur supply on metabolite profiling, glutathione and phytochelatins in Panicum maximum cv. Massai exposed to cadmium

Cited by 39 publications

References 74 publications

Effects of exogenous sulfur on alleviating cadmium stress in tartary buckwheat

Effects of exogenous sulfur on alleviating cadmium stress in tartary buckwheat

Unraveling the mechanisms controlling Cd accumulation and Cd‐tolerance in Brachiaria decumbens and Panicum maximum under summer and winter weather conditions

Transcriptome analysis reveals insight into molecular hydrogen-induced cadmium tolerance in alfalfa: the prominent role of sulfur and (homo)glutathione metabolism

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