A global modulatory role for the Yersinia enterocolitica H-NS protein

Baños, Rosa C.; Pons, José I.; Madrid, Cristina; Juárez, Antonio

doi:10.1099/mic.0.2007/015610-0

Cited by 49 publications

(41 citation statements)

References 46 publications

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“…H-NS family members appear to be essential in several different bacteria (5,18,24,37). In Salmonella, in which the ability of H-NS to repress the expression of genes on pathogenicity islands is key to the fitness of the cell, H-NS does not appear to associate with prophage DNA, presumably because the AT content of these regions is similar to that of the rest of the genome and, thus, not sufficiently high (33).…”

Section: Discussionmentioning

confidence: 99%

Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa

Castang

Dove

2012

J. Bacteriol.

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ABSTRACTMembers of the histone-like nucleoid-structuring (H-NS) family of proteins have been shown to play important roles in silencing gene expression and in nucleoid compaction. InPseudomonas aeruginosa, the two H-NS family members MvaT and MvaU are thought to bind the same AT-rich regions of the chromosome and function coordinately to control a common set of genes. Here we present evidence that the loss of both MvaT and MvaU cannot be tolerated because it results in the production of Pf4 phage that superinfect and kill cells or inhibit their growth. Using a ClpXP-based protein depletion system in combination with transposon mutagenesis, we identify mutants ofP. aeruginosathat can tolerate the depletion of MvaT in an ΔmvaUmutant background. Many of these mutants contain insertions in genes encoding components, assembly factors, or regulators of type IV pili or contain insertions in genes of the prophage Pf4. We demonstrate that cells that no longer produce type IV pili or that no longer produce the replicative form of the Pf4 genome can tolerate the loss of both MvaT and MvaU. Furthermore, we show that the loss of both MvaT and MvaU results in an increase in expression of Pf4 genes and that cells that cannot produce type IV pili are resistant to infection by Pf4 phage. Our findings suggest that type IV pili are the receptors for Pf4 phage and that the essential activities of MvaT and MvaU are to repress the expression of Pf4 genes.

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Section: Discussionmentioning

confidence: 99%

Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa

Castang

Dove

2012

J. Bacteriol.

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“…It was proved that this process is significantly influenced by temperature, but the mechanism is not entirely clear. It is supposed that YmoA is the key regulator of gene expression by environmental factors, including temperature (Madrid et al 2007, Banos et al 2008. It was also proved that the YmoA protein inhibits the expression of the inv gene encoding invasion -an important virulence factor responsible for the transport of bacteria cells through M cells (Ellison et al 2003) and participating in the temperature-dependent production of Yersinia outer proteins (Yops) and Yersinia adhesin (YadA) -plasmid virulence markers (Cornelis et al 1991).…”

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confidence: 99%

“…World literature points to the role of YmoA and related proteins (H-NS) in the regulation of gene expression in various microorganisms, e.g. E. coli, whereas there are only few reports on Y. enterocolitica (Cornelis et al 1991, Ellison et al 2003, Brzostek et al 2007, Banos et al 2008, Fang et al 2008.…”

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confidence: 99%

Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs

Bancerz‐Kisiel¹,

Szczerba‐Turek²,

Platt-Samoraj³

et al. 2012

Polish Journal of Veterinary Sciences

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Yersinia (Y.) enterocolitica is the third etiological agent of human diarrhea in terms of the number of confirmed clinical cases. One of the important virulence markers is the yst gene which encodes the production of enterotoxins Yst (Yersinia stable toxins). However, not all strains with yst genes produce enterotoxins, what seems to be caused by the ymoA gene encoding the production of the YmoA protein inhibiting the expression of various genes. The purpose of our study was to evaluate the distribution of the ymoA and ystA genes and Yst production by Y. enterocolitica isolated from humans and pigs. All the studied strains obtained from pigs had the ystA gene which indicates that they belong to the group of strains commonly regarded as pathogenic, but the ability to produce YstA was detected in only 14 out of 96 examined strains. The fragments of ystA gene were also detected in all Y. enterocolitica strains isolated from human cases of diarrhea. Amplification of a fragment of the ymoA gene was detected in all the studied strains, both from humans and pigs, based on the presence of a 330 bp band. Thus no correlation was identified between the occurrence of the ymoA and ystA genes and the production of a specific type of enterotoxin.

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“…The function of H-NS has been most intensively studied in Salmonella and Escherichia coli, where its ability to bind to intrinsically curved AT-rich DNA has been associated with its role as a global gene regulator (19,21) and more recently as a specific repressor of horizontally acquired genes with atypically low GC content (36,39,42). Most H-NS-like proteins share a conserved C-terminal DNA-binding domain (6), and there is evidence that H-NS-like proteins in other bacteria have a role in global transcriptional control (3,57). In Salmonella and E. coli, H-NS is known to regulate hundreds of genes (36,39,42), whereas in other species, the number of regulated genes may be more restricted (58).…”

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confidence: 99%

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

Bossé

Sinha

et al. 2010

J Bacteriol

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Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-␤-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on the surfaces of polystyrene plates, is lost following serial passage in broth culture, and repeated passage of the nonadherent variants on solid media does not result in reversion to the adherent colony phenotype. In order to investigate the regulation of PGA expression and biofilm formation in A. pleuropneumoniae, we screened a bank of transposon mutants of the nonadherent serovar 1 strain S4074T and identified mutations in two genes, rseA and hns, which resulted in the formation of the adherent colony phenotype. In other bacteria, including the Enterobacteriaceae, H-NS acts as a global gene regulator, and RseA is a negative regulator of the extracytoplasmic stress response sigma factor E . Transcription profiling of A. pleuropneumoniae rseA and hns mutants revealed that both E and H-NS independently regulate expression of the pga operon. Transcription of the pga operon is initiated from a E promoter site in the absence of H-NS, and upregulation of E is sufficient to displace H-NS, allowing transcription to proceed. In A. pleuropneumoniae, H-NS does not act as a global gene regulator but rather specifically regulates biofilm formation via repression of the pga operon. Positive regulation of the pga operon by E indicates that biofilm formation is part of the extracytoplasmic stress response in A. pleuropneumoniae.

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A global modulatory role for the Yersinia enterocolitica H-NS protein

Cited by 49 publications

References 46 publications

Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa

Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa

Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS

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A global modulatory role for the Yersinia enterocolitica H-NS protein

Cited by 49 publications

References 46 publications

Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa

Basis for the Essentiality of H-NS Family Members in Pseudomonas aeruginosa

Distribution of the ymoA and ystA genes and enterotoxins Yst production by Yersinia enterocolitica strains isolated from humans and pigs

Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ E and H-NS

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Regulation of pga Operon Expression and Biofilm Formation in Actinobacillus pleuropneumoniae by σ ^E and H-NS