A glucose biosensor based on the immobilization of glucose oxidase and Au nanocomposites with polynorepinephrine

Liu, Yang; Xu, Nan; Shi, Wei; Liu, Xin; Zi, He; Sun, Yanan; Ge, Dongtao

doi:10.1039/c9ra02054c

Cited by 37 publications

(22 citation statements)

References 28 publications

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“…Green approaches also have been made for the development of glucose biosensors. Yang et al [ 9 ] developed an enzyme electrode based on AuNPs, PNE, and GOx for glucose detection by a green method. The biosensor presented high sensitivity to glucose and high response of fewer than 3 s. Redox mediator p-benzoquinone was added to enhance the linear detection range and sensitivity…”

Section: Enzyme Based Biosensorsmentioning

confidence: 99%

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Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus

et al. 2021

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The use of sensors in critical areas for human development such as water, food, and health has increased in recent decades. When the sensor uses biological recognition, it is known as a biosensor. Nowadays, the development of biosensors has been increased due to the need for reliable, fast, and sensitive techniques for the detection of multiple analytes. In recent years, with the advancement in nanotechnology within biocatalysis, enzyme-based biosensors have been emerging as reliable, sensitive, and selectively tools. A wide variety of enzyme biosensors has been developed by detecting multiple analytes. In this way, together with technological advances in areas such as biotechnology and materials sciences, different modalities of biosensors have been developed, such as bi-enzymatic biosensors and nanozyme biosensors. Furthermore, the use of more than one enzyme within the same detection system leads to bi-enzymatic biosensors or multi-enzyme sensors. The development and synthesis of new materials with enzyme-like properties have been growing, giving rise to nanozymes, considered a promising tool in the biosensor field due to their multiple advantages. In this review, general views and a comparison describing the advantages and disadvantages of each enzyme-based biosensor modality, their possible trends and the principal reported applications will be presented.

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Section: Enzyme Based Biosensorsmentioning

confidence: 99%

“…They are successfully implemented for early disease identification, toxins, viruses, elevated blood levels, etc. [ 6 , 7 , 8 , 9 , 10 ]. In the food industry, biosensors are used to detect food allergens, food contamination, or antioxidant power [ 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus

et al. 2021

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“…Au [30][31][32] and TiO 2 [24,33,34] have been widely applied as transducers in biosensors. This is due to their high stability, biocompatibility, high surface-to-volume ratio, and strong amplification effect on signals.…”

Section: Role Of Tio 2 Nanomaterials In Biosensormentioning

confidence: 99%

State-of-the-Art on Functional Titanium Dioxide-Integrated Nano-Hybrids in Electrical Biosensors

Nadzirah

Gopinath

Parmin

et al. 2020

Critical Reviews in Analytical Chemistry

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Biosensors operating based on electrical methods are being accelerated toward rapid and efficient detection that improve the performance of the device. Continuous study in nano-and material-sciences has led to the inflection with properties of nanomaterials that fit the trend parallel to the biosensor evolution. Advancements in technology that focuses on nano-hybrid are being used to develop biosensors with better detection strategies. In this sense, titanium dioxide (TiO 2) nanomaterials have attracted extensive interest in the construction of electrical biosensors. The formation of TiO 2 nano-hybrid as an electrical transducing material has revealed good results with high performance. The modification of the sensing portion with a combination (nano-hybrid form) of nanomaterials has produced excellent sensors in terms of stability, reproducibility, and enhanced sensitivity. This review highlights recent research advancements with functional TiO 2 nano-hybrid materials, and their victorious story in the construction of electrical biosensors are discussed. Future research directions with commercialization of these devices and their extensive utilizations are also discussed.

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“…Diabetes is determined when the glucose level is above 126 mg/dL in blood without having had a meal for 8 h. It can also be judged when the glucose level is above 200 mg/dL in blood 2 h after a meal [ 3 ]. Among different monitoring options, the electrochemical biosensor is widely used for glucose monitoring, and the electrochemical glucose sensor technology utilizes the glucose oxidase (GOx) enzyme and an electron transfer mediator such as p–benzoquinone, phenazine ethosulfate, lutetium phthalocyanine, or osmium [ 4 , 5 , 6 , 7 , 8 , 9 ]. Commercial blood glucose monitoring devices on the market mainly utilize a second-generation glucose sensor that consists of a metal mediator and GOx enzyme [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a Glucose Sensor Based on Glucose Dehydrogenase Using Polydopamine-Functionalized Nanotubes

2021

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The electrochemical-based detection of glucose is widely used for diagnostic purposes and is mediated by enzyme-mediated signal transduction mechanisms. For such applications, recent attention has focused on utilizing the oxygen-insensitive glucose dehydrogenase (GDH) enzyme in place of the glucose oxidase (GOx) enzyme, which is sensitive to oxygen levels. Currently used Ru-based redox mediators mainly work with GOx, while Ru(dmo–bpy)2Cl2 has been proposed as a promising mediator that works with GDH. However, there remains an outstanding need to improve Ru(dmo–bpy)2Cl2 attachment to electrode surfaces. Herein, we report the use of polydopamine-functionalized multi-walled carbon nanotubes (PDA-MWCNTs) to effectively attach Ru(dmo–bpy)2Cl2 and GDH onto screen-printed carbon electrodes (SPCEs) without requiring a cross–linker. PDA-MWCNTs were characterized by Fourier transform infrared (FT–IR) spectroscopy, Raman spectroscopy, and thermal gravimetric analysis (TGA), while the fabrication and optimization of Ru(dmo–bpy)2Cl2/PDA-MWCNT/SPCEs were characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) measurements. The experimental results demonstrate a wide linear range of glucose-concentration-dependent responses and the multi-potential step (MPS) technique facilitated the selective detection of glucose in the presence of physiologically relevant interfering species, as well as in biological fluids (e.g., serum). The ease of device fabrication and high detection performance demonstrate a viable pathway to develop glucose sensors based on the GDH enzyme and Ru(dmo–bpy)2Cl2 redox mediator and the sensing strategy is potentially extendable to other bioanalytes as well.

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A glucose biosensor based on the immobilization of glucose oxidase and Au nanocomposites with polynorepinephrine

Abstract: The PNE/GOD/AuNPs@PNE/Au electrode exhibited a low Michaelis–Menten constant, a fast response to glucose, outstanding anti-interference ability and high sensitivity.

Cited by 37 publications

References 28 publications

Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus

Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus

State-of-the-Art on Functional Titanium Dioxide-Integrated Nano-Hybrids in Electrical Biosensors

Development of a Glucose Sensor Based on Glucose Dehydrogenase Using Polydopamine-Functionalized Nanotubes

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