A glucose/mannose binding lectin from litchi ( Litchi chinensis ) seeds: Biochemical and biophysical characterizations

Bose, Partha Pratim; Bhattacharjee, Surajit; Singha, Shuvendu; Mandal, Santanu; Mondal, Gautam; Gupta, Priya; Chatterjee, Bishnu P.

doi:10.1016/j.bbrep.2016.05.001

Cited by 13 publications

(6 citation statements)

References 44 publications

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“…Notably, the role of annexin protein in abiotic stress management is well characterized [ 44 ]; however, its role in biotic stress is yet to be examined. Likewise, upregulations of transcripts encoding the Raf kinase inhibitor family, lipid transfer EARLI 1-like, senescence-induced receptor-like serine threonine-kinases, cathepsin B-like protease 2 and mannose glucose-binding lectin were involved in the innate immunity and PCD [ 28 , 29 , 30 , 45 , 46 ]. This indicates that these transcripts might also play a central role in the conferring resistance to U 4-7-5 against aflatoxin contamination.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, in the model plant Arabidopsis, involvement of myo-inositol metabolism with PCD and basal immunity is well demonstrated. Reduced levels of inositol can induce severe cell death and disease symptoms by suppressing/knockdown of inositol-3-phosphate synthase [ 47 ] and myo-inositol-1-phosphate synthase [ 46 ]. Wild type phenotype can be restored by feeding these inositol deficient mutants or transgenics with inositol/galactinol [ 47 , 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Soni

Nayak

Kumar

et al. 2020

JoF

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Aflatoxin-affected groundnut or peanut presents a major global health issue to both commercial and subsistence farming. Therefore, understanding the genetic and molecular mechanisms associated with resistance to aflatoxin production during host–pathogen interactions is crucial for breeding groundnut cultivars with minimal level of aflatoxin contamination. Here, we performed gene expression profiling to better understand the mechanisms involved in reduction and prevention of aflatoxin contamination resulting from Aspergillus flavus infection in groundnut seeds. RNA sequencing (RNA-Seq) of 16 samples from different time points during infection (24 h, 48 h, 72 h and the 7th day after inoculation) in U 4-7-5 (resistant) and JL 24 (susceptible) genotypes yielded 840.5 million raw reads with an average of 52.5 million reads per sample. A total of 1779 unique differentially expressed genes (DEGs) were identified. Furthermore, comprehensive analysis revealed several pathways, such as disease resistance, hormone biosynthetic signaling, flavonoid biosynthesis, reactive oxygen species (ROS) detoxifying, cell wall metabolism and catabolizing and seed germination. We also detected several highly upregulated transcription factors, such as ARF, DBB, MYB, NAC and C2H2 in the resistant genotype in comparison to the susceptible genotype after inoculation. Moreover, RNA-Seq analysis suggested the occurrence of coordinated control of key pathways controlling cellular physiology and metabolism upon A. flavus infection, resulting in reduced aflatoxin production.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Soni

Nayak

Kumar

et al. 2020

JoF

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“…ex Baker Zingiberaceae Rhizome [77] Non-specified HIV-1, HCV, HCMV [78] Lathyrus sativus L. Fabaceae Seed [79] Mannose Not reported Litchi chinensis Sonn. Sapindaceae Seed [80] Glucose/mannose HSV-1 [81] Mangifera indica L. Anacardiaceae Fruit seed [82] Non-specified HSV, influenza virus [83] , [84] Momordica charantia L Cucurbitaceae Seed [85] Galactose/ N-Acetylgalactosamine HIV, HSV-1, H1N1, H3N2, H5N1 [86] , [87] , [88] Mucuna pruriens (L.) DC. Fabaceae Seed [89] Mannose HCV [90] Musa paradisiaca L. Musaceae Ripe fruit pulp [91] Mannose HSV-1, HSV-2 [91] Oryza sativa L. Poaceae – [82] N-Acetylglucosamine CMV, HSV [92] , [93] Phaseolus vulgaris L. Fabaceae Seed [94] , [95] Galactose HIV-1 RT [94] , [95] Psidium guajava L. Myrtaceae <...>…”

Section: Introductionmentioning

confidence: 99%

Plant lectins as prospective antiviral biomolecules in the search for COVID-19 eradication strategies

Ahmed

Jahan

Nissapatorn

et al. 2022

Biomedicine & Pharmacotherapy

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Lectins or clusters of carbohydrate-binding proteins of non-immune origin are distributed chiefly in the Plantae. Lectins have potent anti-infectivity properties for several RNA viruses including SARS-CoV-2. The primary purpose of this review is to review the ability of lectins mediated potential biotherapeutic and bioprophylactic strategy against coronavirus causing COVID-19. Lectins have binding affinity to the glycans of SARS-COV-2 Spike glycoprotein that has N-glycosylation sites. Apart from this, the complement lectin pathway is a “first line host defense” against the viral infection that is activated by mannose-binding lectins. Mannose-binding lectins deficiency in serum influences innate immunity of the host and facilitates infectious diseases including COVID-19. Our accumulated evidence obtained from scientific databases particularly PubMed and Google Scholar databases indicate that mannose-specific/mannose-binding lectins (MBL) have potent efficacies like anti-infectivity, complement cascade induction, immunoadjuvants, DC-SIGN antagonists, or glycomimetic approach, which can prove useful in the strategy of COVID-19 combat along with the glycobiological aspects of SARS-CoV-2 infections and antiviral immunity. For example, plant-derived mannose-specific lectins BanLac, FRIL, Lentil, and GRFT from red algae can inhibit and neutralize SARS-CoV-2 infectivity, as confirmed with in-vitro , in-vivo , and in-silico assessments. Furthermore, Bangladesh has a noteworthy resource of antiviral medicinal plants as well as plant lectins. Intensifying research on the antiviral plant lectins, adopting a glyco-biotechnological approach, and with deeper insights into the “glycovirological” aspects may result in the designing of alternative and potent blueprints against the 21st century's biological pandemic of SARS-CoV-2 causing COVID-19

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“…The ability of lectins to selectively agglutinate red blood cells is utilized for human and animal blood group typing and subtyping [14–16]. Hemagglutination (HA) or yeast agglutination assay (YA) is used to search for new lectins of various organisms [17, 18] as well as for the rapid testing of lectin activity [19]. The agglutination of microbial cells often serves for studying the interactions between lectins and pathogens [20].…”

Section: Introductionmentioning

confidence: 99%

“…Human red blood cells and yeast cells were used in agglutination assays, because their cell surface glycans contain terminal l -fucose and d -mannose, respectively. Both types of cells are commonly utilized for the determination of lectin specificity [19, 27] and are readily available. In addition, a simple portable bright-field microscope can be used for the observation of agglutinates.…”

Section: Introductionmentioning

confidence: 99%

Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins

2019

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Lectins are a group of ubiquitous proteins which specifically recognize and reversibly bind sugar moieties of glycoprotein and glycolipid constituents on cell surfaces. The mutagenesis approach is often employed to characterize lectin binding properties. As lectins are not enzymes, it is not easy to perform a rapid specificity screening of mutants using chromogenic substrates. It is necessary to use different binding assays such as isothermal titration calorimetry (ITC), surface plasmon resonance (SPR), microscale thermophoresis (MST), enzyme-linked lectin assays (ELLA), or glycan arrays for their characterization. These methods often require fluorescently labeled proteins (MST), highly purified proteins (SPR) or high protein concentrations (ITC). Mutant proteins may often exhibit problematic behaviour, such as poor solubility or low stability. Lectin-based cell agglutination is a simple and low-cost technique which can overcome most of these problems. In this work, a modified method of the agglutination of human erythrocytes and yeast cells with microscopy detection was successfully used for a specificity study of the newly prepared mutant lectin RS-IIL_A22S, which experimentally completed studies on sugar preferences of lectins in the PA-IIL family. Results showed that the sensitivity of this method is comparable with ITC, is able to determine subtle differences in lectin specificity, and works directly in cell lysates. The agglutination method with microscopy detection was validated by comparison of the results with results obtained by agglutination assay in standard 96-well microtiter plate format. In contrast to this assay, the microscopic method can clearly distinguish between hemagglutination and hemolysis. Therefore, this method is suitable for examination of lectins with known hemolytic activity as well as mutant or uncharacterized lectins, which could damage red blood cells. This is due to the experimental arrangement, which includes very short sample incubation time in combination with microscopic detection of agglutinates, that are easily observed by a small portable microscope.

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A glucose/mannose binding lectin from litchi ( Litchi chinensis ) seeds: Biochemical and biophysical characterizations

Cited by 13 publications

References 44 publications

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism upon Aspergillus flavus Infection Resulting in Reduced Aflatoxin Production in Groundnut

Plant lectins as prospective antiviral biomolecules in the search for COVID-19 eradication strategies

Microscopy examination of red blood and yeast cell agglutination induced by bacterial lectins

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