A Glycosyltransferase from Arabidopsis thaliana Enables the Efficient Enzymatic Synthesis of Gastrodin

Xia, Hui; Zhang, Zihan; Ding, Jingzhong; Jiang, Keji; Xue, Feng

doi:10.1007/s10562-023-04406-y

Cited by 2 publications

(1 citation statement)

References 34 publications

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“…coli) and Saccharomyces cerevisiae (S. cerevisiae), have been used to produce gastrodin. − Notably, the Liu group has established a novel microbial pathway for gastrodin synthesis from glucose by incorporating Nocardia carboxylic acid reductase (CAR), Bacillus subtilis phosphopantetheinyl transferase (SFP), endogenous alcohol dehydrogenases (ADHs), and Rhodiola glycosyltransferase UGT73B6. Finally, after genetic modification via metabolic and protein engineering, the engineered E.…”

Section: Introductionmentioning

confidence: 99%

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Wu,

Li,

Sun

et al. 2024

ACS Synth. Biol.

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Gastrodin, 4-hydroxybenzyl alcohol-4-O-β-D-glucopyranoside, has been widely used in the treatment of neurogenic and cardiovascular diseases. Currently, gastrodin biosynthesis is being achieved in model microorganisms. However, the production levels are insufficient for industrial applications. In this study, we successfully engineered a Yarrowia lipolytica strain to overproduce gastrodin through metabolic engineering. Initially, the engineered strain expressing the heterologous gastrodin biosynthetic pathway, which comprises chorismate lyase, carboxylic acid reductase, phosphopantetheinyl transferase, endogenous alcohol dehydrogenases, and a UDP-glucosyltransferase, produced 1.05 g/L gastrodin from glucose in a shaking flask. Then, the production was further enhanced to 6.68 g/L with a productivity of 2.23 g/L/day by overexpressing the key node DAHP synthases of the shikimate pathway and alleviating the native tryptophan and phenylalanine biosynthetic pathways. Finally, the best strain, Gd07, produced 13.22 g/L gastrodin in a 5 L fermenter. This represents the highest reported production of gastrodin in an engineered microorganism to date, marking the first successful de novo production of gastrodin using Y. lipolytica.

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Section: Introductionmentioning

confidence: 99%

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Wu,

Li,

Sun

et al. 2024

ACS Synth. Biol.

View full text Add to dashboard Cite

show abstract

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Wu,

Li,

Sun

et al. 2024

Preprint

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Gastrodin, 4-hydroxybenzyl alcohol-4-O-β-D-glucopyranoside, has been widely used in the treatment of neurogenic and cardiovascular diseases. Currently, gastrodin biosynthesis has been achieved in model microorganisms. However, the production levels are insufficient for industrial applications. In this study, we successfully engineered a Yarrowia lipolytica strain to overproduce gastrodin through metabolic engineering. Initially, the engineered strain expressing the heterologous gastrodin biosynthetic pathway, which comprises chorismate lyase, carboxylic acid reductase, phosphopantetheinyl transferase, endogenous alcohol dehydrogenases, and a UDP-glucose dependent glucosyltransferase, produced 1.05 g/L of gastrodin from glucose in a shaking flask. Then, the production was further enhanced to 6.68 g/L with a productivity of 2.23 g/L/day by over-expressing the key node DAHP synthases of the shikimate pathway and alleviating the native tryptophan and phenylalanine biosynthetic pathways. Finally, the best strain, Gd07, produced 13.22 g/L of gastrodin in a 5-L fermenter. This represents the highest reported production of gastrodin in an engineered microorganism to date, marking the first successful de novo production of gastrodin using Y. lipolytica.

show abstract

A Glycosyltransferase from Arabidopsis thaliana Enables the Efficient Enzymatic Synthesis of Gastrodin

Cited by 2 publications

References 34 publications

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

Enhancing Gastrodin Production in Yarrowia lipolytica by Metabolic Engineering

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