A glyphosate-based herbicide induces histomorphological and protein expression changes in the liver of the female guppy Poecilia reticulata

Santos, Alex Caetano dos; Rocha, Thiago Lopes; Borges, Clayton Luiz; Bailão, Alexandre Melo; Soares, Célia Maria de Almeida; Sabóia‐Morais, Simone Maria Teixeira de

doi:10.1016/j.chemosphere.2016.10.116

Cited by 47 publications

(17 citation statements)

References 71 publications

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“…All together, these results demonstrate a serious hepatic suffering and are in agreement with those retrieved by studies carried out in fish [38–40]. However, the results achieved in P. siculus liver are sex-independent, whereas in the fish Poecilia reticulata the Gly-induced hepatic diseases are more common in males [41].…”

Section: Discussionsupporting

confidence: 88%

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

Verderame

Scudiero

2019

BioMed Research International

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The potential toxicity of glyphosate, a widely used broad-spectrum herbicide, is currently a great matter of debate. As vertebrate insectivores, lizards protect plants from herbivorous insects increasing plant biomass via the trophic cascade and represent an important link between invertebrates and higher predators. A negative effect of glyphosate on lizards’ survival could have major impacts at the ecological levels. In this study, we investigated the effects of the exposure to low doses of glyphosate on the liver of the wall lizard Podarcis siculus, a suitable bioindicator of soil pollution. Two different doses of pure glyphosate (0.05 and 0.5 μg/kg body weight) were orally administered every other day for 3 weeks to sexually mature males and females. The results demonstrated that both doses, despite being very low, are toxic for the liver that showed clear signs of suffering, regardless of sex. The histological analysis provided a scenario of severe hepatic condition, which degenerated until the appearance of fibrotic formations. The morphological observations were consistent with a loss of liver physiological functions. Immunocytochemical investigations allowed us to detect an involvement of antioxidant/cytoprotective proteins, such as superoxide dismutase 1 (Cu/Zn SOD, known as SOD1), glutathione peroxidase 1 (GPx1), metallothionein (MT), and tumor suppressor protein 53, (p53) suggesting that the liver was trying to react against stress signals and damage induced by glyphosate. Finally, in situ hybridization and Real-Time PCR analysis showed the upregulation of estrogen receptor α and vitellogenin gene expression, thus demonstrating the xenoestrogenic action of glyphosate. The imbalance of the hormonal homeostasis could threaten the lizards’ reproductive fitness and survival, altering the trophic cascade.

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Section: Discussionsupporting

confidence: 88%

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

Verderame

Scudiero

2019

BioMed Research International

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“…Although numerous biomarkers have been used to evaluate the effects of glyphosate on amphibians, new diagnostic tools are needed particularly in relation to the organs that play a vital role in the processes of detoxification such as the liver. In this sense, the effects of glyphosate on the histology of fish (Bawa et al, 2017;dos Santos Rezende et al, 2017;Hued et al, 2012;Jiraungkoorskul et al, 2003;Ne skovi c et al, 1996;Shiogiri et al, 2012) and mammalian (Benedetti et al, 2004;Ça glar and Kolankaya, 2008;Larsen et al, 2012;Malatesta et al, 2008) liver were evaluated, but little information is available in anurans (Perez-Iglesias et al, 2016). The liver plays a fundamental role in the biotransformation processes of xenobiotics, which in ectotherms, involve both hepatocytes and melanomacrophagic cells (MMc) (Fenoglio et al, 2005;Steinel and Bolnick, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of glyphosate and its commercial formulation, Roundup® Ultramax, on liver histology of tadpoles of the neotropical frog, Leptodactylus latrans (amphibia: Anura)

et al. 2018

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In the last years, the agricultural expansion has led to an increased use of pesticides, with glyphosate as the most widely used worldwide. This is also the situation in Argentina, where glyphosate formulations are the most commercialized herbicides. It is known that glyphosate formulations are much more toxic than the active ingredient, and this difference in toxicity can be attributed to the adjuvants present in the formula. In this context, the aim of the present study was to evaluate and compare sub-lethal histological effects of the glyphosate formulation Roundup Ultramax and glyphosate active ingredient on Leptodactylus latrans tadpoles at Gosner-stage 36. Semi-static bioassays were performed using 96 h of exposure with Roundup Ultramax formulation (RU; 0.37-5.25 mg a.e./L), glyphosate (GLY; 3-300 mg/L), and a control group. RU exposure showed an increment in the melanomacrophagic cells (MMc) and melanomacrophagic centers (MMCs) from 0.37 mg a.e./L. GLY exposure showed a significant increment in the number of MMc from 15 mg/L, and of MMCs from 3 mg/L. Also, histopathological lesions were observed in the liver of tadpoles exposed to both, GLY and RU. These lesions included: lipidosis and hepatic congestion, but only RU showed significant differences respect to control, with a LOEC value of 2.22 mg a.e./L for both effects. In sum, this study represents the first evidence of adverse effects of glyphosate and RU formulation on the liver of anuran larvae at concentrations frequently found in the environment.

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“…The fish Poecilia sp., well-known as a guppy, has been used as a model system in ecotoxicological studies due to a high sensitivity towards different pollutants, as well as the small, cheap and easy management in aquariums (Araújo et al, 2001;Dorrington et al, 2012;Sabóia-Moraes et al, 1996;Silva et al, 2002). Recent studies on the GBH toxicity (Rocha et al, 2015;Santos et al, 2017;Souza Filho et al, 2013) together with other ecotoxicological studies (Castro et al, 2004;Rolshausen et al, 2015) chose Poecilia reticulata as the model system. P. reticulata has morphological and physiological differences between genders, such as small size and intense coloring in males compared to females, different digestive enzyme profiles and nutritional response (Thongprajukaew & Kovitvadhi, 2013).…”

Section: Introductionmentioning

confidence: 99%

Gender‐specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

Antunes

Rocha

Pires

et al. 2017

J of Applied Toxicology

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Ecotoxicity of glyphosate (GLY) and its metabolite aminomethylphosphonic acid (AMPA) was investigated in guppies, Poecilia reticulata. We tested the effects of these chemicals on the gills and liver of both male and female guppies using qualitative and quantitative histopathological analyses associated with histopathological condition indexes. Both genders showed similar median lethal concentration (LC ) at 96 h for GLY (68.78 and 70.87 mg l ) and AMPA (180 and 164.32 mg l ). However, the histopathological assessment of both fish organs exposed to sublethal concentrations of GLY (35 mg l ) and AMPA (82 mg l ) for 96 h showed a tissue- and gender-specific histopathological response. In both exposure assays, fish presented mainly progressive changes, such as proliferation of the interlamellar epithelium, partial and total fusion of secondary lamellae. The liver showed mainly regressive changes, such as steatosis, pyknotic nuclei and high distribution of collagen fibers. Unusually large hepatocytes as degenerated cells were also detected. Histopathological changes in gills were similar for the males and females, but the liver response was different between the genders. The hepatic inflammatory changes were more common in males. The increase in the area of hepatocyte vacuoles is gender dependent with higher values in the male compared to the female guppies exposed to GLY and AMPA. Multiparametric analysis indicated that the male guppies are more sensitive than females, particularly in the presence of AMPA. Our study shows that the histopathological assessment associated with gender-specific response can be successfully used in ecotoxicological assessment of GLY and the metabolite AMPA. Copyright © 2017 John Wiley & Sons, Ltd.

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A glyphosate-based herbicide induces histomorphological and protein expression changes in the liver of the female guppy Poecilia reticulata

Cited by 47 publications

References 71 publications

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

How Glyphosate Impairs Liver Condition in the Field Lizard Podarcis siculus (Rafinesque-Schmaltz, 1810): Histological and Molecular Evidence

Effects of glyphosate and its commercial formulation, Roundup® Ultramax, on liver histology of tadpoles of the neotropical frog, Leptodactylus latrans (amphibia: Anura)

Gender‐specific histopathological response in guppies Poecilia reticulata exposed to glyphosate or its metabolite aminomethylphosphonic acid

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