A Gold Nanoparticle Bouquet held on plasma membrane: An ultrasensitive dark-field imaging approach for Cancer Cell Analysis

Abstract

Rational: p53 is suppressing tumor protein correlated with the cell cycle factors and apoptosis. Here, a gold nanoparticle bouquet is designed for an ultrasensitive dark-field imaging approach for cancer cell analysis. Methods: AuNP60/APBA is functionalized by a gold nanoparticle bouquet-plasmonic 60 nm gold nanoparticles. And consistent APBA can be held on the plasma membrane. After 13 nm gold nanoparticles are functionalized with mannose (AuNP13/MN), the AuNP60/APBA gold nanoparticles are captured. The absorption spectrum of aggregation gold nanoparticles (AuNPs) shifts to near-infrared (NIR) region which can be observed under dark-field microscopy (DFM) and is treated the subsequent with photothermal therapy. Results:The results that MCF-7 cells were successfully destroyed under the near-infrared (NIR) irradiation and the intracellular WTp53 increased while the MTp53 decreased. These results indicated that p53 is the key molecule in the apoptosis signaling pathway. Photothermal therapy can stimulate the MTp53 in the cell signal conductive pathway. Conclusion: This work offers a new method for intracellular p53 analysis and a potential targeted cancer treatment.

Protein-coated nanoparticles exhibit Lévy flights on a suspended lipid bilayer

“…Depending on NP motion, they can cluster on the cell surface, be internalized, or return to the host phase. 10…”

“…Depending on NP motion, they can cluster on the cell surface, be internalized, or return to the host phase. 10 With recent developments in microscopy techniques, it is now possible to visualize individual NPs and track the diffusion of these NPs at the surface of bilayers. 11 The resulting trajectories can occasionally be characterized by a classical two-dimensional random walk, 12 but are often reported as anomalous diffusion, where their diffusive behaviour evolves over time.…”

Noninvasive Imaging of Cancer Immunotherapy

“…Recent nanotheranostic therapeutic advancements include the development of a new liquid brachytherapy approach via novel cationic micelle and liposome formulations 175 , and the use of cross-linked iron oxide nanoparticles conjugated to azademethylcolchicine (CLIOT-ICT) as a method to eradicate a subpopulation of quiescent glioblastoma initiating cells 176 . Another recent theranostic advancement is the analysis of cancer cells by ultrasensitive dark-field imaging using gold nanoparticle bouquets 177 . With a realistic mind regarding a timeline for clinical translation, the above-mentioned approaches may need some additional time to achieve regulatory approval, but represent examples of the next generation of multi-functional agents that are certainly in the pipeline, most of which also make use at least in part of radioimaging or radiotherapy.…”

Recent Advances in Noble Metal Nanoparticles for Cancer Nanotheranostics

“…Breast cancer suppression using NIR photothermal therapy (PTT) in the presence of a bouquet of 13 nm AuNPs linked with mannose-polysaccharide over 60 nm AuNPs was studied. The uptake of AuNPs by MCF-7 cells in vitro and BALB/c nude tumor mice in vivo model resulted in an increase in WTp53 protein and tumor cell death when exposed to NIR 680 nm laser for photothermal therapy and monitored by dark-field imaging [28]. In another study on a photothermal therapeutic approach for breast cancer, an AuNPs-aptamer-graphene oxide hybridized system modulated heat shock protein expression in selectively targeted MCF-7 cells in vitro [29].…”