A Gold Nanoparticles Enhanced Surface Plasmon Resonance Immunosensor for Highly Sensitive Detection of Ischemia-Modified Albumin

Abstract

In this study a novel sensitive nanogold particle sensor enhancement based on mixed self-assembled monolayers was explored and used to construct a Surface Plasmon Resonance (SPR) immunosensor to detect Ischemia Modified Albumin (IMA). Compared with a direct binding SPR assay at a limit of detection (LOD) of 100 ng/L, gold nanoparticles (AuNPs) of 10 nm dramatically improved the LOD of IMA to 10 ng/L. Meanwhile, no interfering substance that may lead to false positive results was identified. These results suggested that the SPR biosensor presented superior properties, and provided a simple label-free strategy to increase assay sensitivity for further acute coronary syndrome (ACS) diagnosis.

Measuring ultra-low levels of nucleotide biomarkers using quartz crystal microbalance and SPR microarray imaging methods: A comparative analysis

“…The use of gold nanoparticles (AuNPs) has been shown to increase the reflectivity changes of a hybridization reaction due to surface plasmon signal enhancement. 25,26,27 …”

SPR detection of cardiac troponin T for acute myocardial infarction

“…The binding response with subsequent injection of the AuNP modified detector antibody showed an approximate three fold signal amplification. The use of AuNPs conjugated to antibodies to enhance the response has been widely used and reported [34][35][36][37][38]. The actual cTnT detection antibody binding responses were obtained after subtraction of the non-specific binding response obtained for the control antibody (Fig.…”

Comparative evaluation of healing biomarkers in skin wound exudates using a nanobiosensor and histological analysis of full‐thickness skin wounds grafted with multidirectional or unidirectional artificial dermis

“…The gold surface of CM5 chips is coated with carboxymethylated dextran, which in spite of increasing the immobilization of the ligand can adsorb organic compounds that lead to non‐specific binding during the quantification of molecules that are part of biological fluids or media isolated from cultured cells (Battaglia et al, ; GE Healthcare, , accessed June 8, 2018). A carboxymethylated dextran matrix can also enlarge the distance between the analyte and the ligand attached to the chip surface weakening the interactions between them and leading to a decrease in signal (Li et al, ). For that reason, the use of CM5 chips in the analysis of complex biological fluids—such as wound exudates—remains a challenge (Battaglia et al, ; Chou, Chuang, & Wu, ; Nguyen et al, ; Wink, van Zuilen, Bult, & van Bennekom, ) and requires standardizing a very sensitive SPR methodology.…”