A Golgi study of the neuronal population of the marginal zone (lamina I) of the rat spinal cord

Lima, Deolinda; Coimbra, Antonio

doi:10.1002/cne.902440105

Cited by 163 publications

(177 citation statements)

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“…The dendrites could be followed easily for up to 400 m or more. Consistent with previous observations in rat, cat, and monkey (Gobel, 1978;Price et al, 1978;Beal et al, 1981;Lima and Coimbra, 1986;, CTb-labeled lamina I STT cells in the monkey arborize in the horizontal plane. Most cells were oriented longitudinally, and their primary dendritic arbors were nearly always restricted to lamina I; few cells were observed with a dendrite extending into lamina II.…”

Section: Resultssupporting

confidence: 91%

“…First, previous retrograde labeling studies in cat and rat have produced conflicting results. Even though Golgi studies in cat (Gobel, 1978) and rat (Lima and Coimbra, 1986) both described similar cell types in lamina I (fusiform, pyramidal, and multipolar), a retrograde labeling study of lamina I STT cells in the rat reported that they were mostly pyramidal cells (Lima and Coimbra, 1988), whereas a comparable study in the cat found fusiform, pyramidal, and multipolar lamina I STT cells . Both studies utilized cholera toxin subunit b (CTb), which provides excellent retrograde morphological definition (Ericson and Blomqvist, 1988).…”

mentioning

confidence: 98%

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Morphology and Distribution of Spinothalamic Lamina I Neurons in the Monkey

Zhang

Craig

1997

J. Neurosci.

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Lamina I spinothalamic tract (STT) neurons were identified by retrograde labeling with cholera toxin subunit b (CTb) in monkeys. On the basis of the criteria of somatal shape and dendritic orientation in horizontal sections used in prior work in the cat, three distinct morphological types were recognized: fusiform (F) cells with spindle-shaped somata and two main longitudinal dendritic arbors; pyramidal (P) cells with triangular somata and three main dendrites oriented primarily longitudinally; and multipolar (M) cells with polygonal somata and four or more dendrites directed longitudinally and mediolaterally. Some cells had transitional shapes, but cells with indeterminate shapes and a few with small round, unipolar, or eccentric somata were grouped as unclassified (U). Greater variation appeared in the monkey than had been seen in the cat, and more subtypes were noted. The overall proportions of these cell types were: 47% F, 27% P, 22% M, and 5% U. Differential longitudinal distributions were found over the length of the spinal cord (from the second cervical through the first coccygeal segments). Pyramidal and multipolar cells together predominated in the enlargements, whereas fusiform cells predominated in thoracic segments. We conclude that three distinct morphological types of lamina I STT cells are present in the monkey as in the cat. Considered with other recent findings, the present results support the possibility that these three cell types may correspond to distinct physiological classes of nociceptive and thermoreceptive lamina I STT cells.

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Section: Resultssupporting

confidence: 91%

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confidence: 98%

Morphology and Distribution of Spinothalamic Lamina I Neurons in the Monkey

Zhang

Craig

1997

J. Neurosci.

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“…The rest of the cells were next to the lateral edge of lamina I, among the superficial fibres of the DLF (n=18; cells 6-23) or within the LSN (n=9; cells 24-32). While neurons within lamina I could be classified according to the system introduced by Lima and Coimbra (1986), neurons located more laterally, outside the dorsal grey matter and in the LSN, with the exception of three cells (Table 1) presented very different somatodendritic features. We compared basic morphometric parameters of these neurons with a pooled sample of 3-D reconstructed PNs and LCNs (n=4 for each, L-I in Fig.…”

Section: General Somatodendritic Features Of Neurons Outside the Dorsmentioning

confidence: 99%

“…position soma / dendrite (Lima and Coimbra, 1986) midline Table 1 dorsolateral funiculus; DF, dorsal funiculus; L-I-X, lamina I-X; 3-D, neuron reconstructed in three-dimensions with Neurolucida; DCT, dorsal collateral type projection neuron (see Szucs et al, 2010); MCT, mixed collateral type projection neuron (see Szucs et al, 2010);…”

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confidence: 99%

Neurons in the lateral part of the lumbar spinal cord show distinct novel axon trajectories and are excited by short propriospinal ascending inputs

et al. 2015

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Order of Authors Secondary Information:The role of spinal dorsal horn propriospinal connections in nociceptive processing is not yet established. Recently described, rostrocaudally oriented axon collaterals of lamina I projection and local-circuit neurons (PNs and LCNs) running in the dorsolateral funiculus (DLF) may serve as the anatomical substrate for intersegmental processing. Putative targets of these axons include lateral dendrites of superficial dorsal horn neurons, including PNs, and also neurons in the lateral spinal nucleus (LSN) that are thought to be important integrator units receiving, among others, visceral sensory information. Here we used an intact spinal cord preparation to study intersegmental connections within the lateral part of the superficial dorsal horn. We detected brief monosynaptic and prolonged polysynaptic excitation of lamina I and LSN neurons when stimulating individual dorsal horn neurons located caudally, even in neighbouring spinal cord segments. These connections, however, were infrequent. We also revealed that some projection neurons outside the dorsal grey matter and in the LSN have distinct, previously undescribed course of their projection axon. Our findings indicate that axon collaterals of lamina I PNs and LCNs in the DLF rarely form functional connections with other lamina I and LSN neurons and that the majority of their targets are on other elements of the dorsal horn. The unique axon trajectories of neurons in the dorsolateral aspect of the spinal cord, including the LSN do not fit our present understanding of midline axon guidance and suggest that their function and development differ from the neurons inside lamina I. These findings emphasize the importance of understanding the connectivity matrix of the superficial dorsal horn in order to decipher spinal sensory information processing. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporation Answers to Reviewer 1First of all, the authors would like to thank Reviewer 1 for his constructive comments and suggestions that helped us improving the manuscript. Below we give detailed answers to all major and minor concerns raised. Major issues 1)We completely agree with the reviewer that the statistical analysis would have helped to make our conclusions on morphometric parameters stronger. The reason why we decided to talk about the tendencies only without statistical tests was the low number of the reconstructed neurons in the groups. However, since we observed and wanted to demonstrate the difference between neurons within and outside lamina I (in the adjacent lateral white matter), in this revised version we pooled projection neurons (PNs) and localcircuit neurons (LCNs) from our previous reports to form a group of cells in lamina I called "L-I" whereas neurons lateral to the edge of the dorsal horn and in the LSN are now referred to as "OUT/LSN". Statistical analyses were performed between these two groups and showed significant differences in the morphometric parameters investigated, with the...

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“…Morphological and functional studies have revealed a tremendous diversity of dorsal horn neurons (Christensen and Perl, 1970;Lima and Coimbra, 1986;Todd and Spike, 1993;Han et al, 1998;Grudt and Perl, 2002;Todd and Koerber, 2006). Diversity of the dorsal horn neurons is further suggested by the expression of neuropeptides, including the opioid-like peptides Dynorphin (DYN) and enkephalin (ENK), the anti-opioid peptide cholecystokinin (CCK), the tachykinin peptides Substance P (SP) and Neurokinin B (NKB), somatostatin (SOM), and others (Marti et al, 1987;Todd and Spike, 1993;Todd and Koerber, 2006;Polgar et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Tlx1 and Tlx3 Coordinate Specification of Dorsal Horn Pain-Modulatory Peptidergic Neurons

Xu¹,

Lopes²,

Qian³

et al. 2008

J. Neurosci.

113

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The dorsal spinal cord synthesizes a variety of neuropeptides that modulate the transmission of nociceptive sensory information. Here, we used genetic fate mapping to show that Tlx3 ϩ spinal cord neurons and their derivatives represent a heterogeneous population of neurons, marked by partially overlapping expression of a set of neuropeptide genes, including those encoding the anti-opioid peptide cholecystokinin, pronociceptive Substance P (SP), Neurokinin B, and a late wave of somatostatin. Mutations of Tlx3 and Tlx1 result in a loss of expression of these peptide genes. Brn3a, a homeobox transcription factor, the expression of which is partly dependent on Tlx3, is required specifically for the early wave of SP expression. These studies suggest that Tlx1 and Tlx3 operate high in the regulatory hierarchy that coordinates specification of dorsal horn pain-modulatory peptidergic neurons.

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A Golgi study of the neuronal population of the marginal zone (lamina I) of the rat spinal cord

Cited by 163 publications

References 45 publications

Morphology and Distribution of Spinothalamic Lamina I Neurons in the Monkey

Morphology and Distribution of Spinothalamic Lamina I Neurons in the Monkey

Neurons in the lateral part of the lumbar spinal cord show distinct novel axon trajectories and are excited by short propriospinal ascending inputs

Tlx1 and Tlx3 Coordinate Specification of Dorsal Horn Pain-Modulatory Peptidergic Neurons

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