A gradient four-layered gelatin methacrylate/agarose construct as an injectable scaffold for mimicking osteochondral tissue

Mokhtarzade, Ali; Imani, Rana; Shokrollahi, Parvin

doi:10.1007/s10853-023-08374-x

Cited by 8 publications

(4 citation statements)

References 98 publications

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“…Moreover, the layers displayed suitable injectability. Cytotoxicity assessments demonstrated the viability of cells exceeding 91% and 86% after 1 and 7 d, respectively [153].…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Pramanik,

Alhomrani,

Alamri

et al. 2024

Biomed. Mater.

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Gelatin methacryloyl (GelMA) hydrogels have gained significant recognition as versatile biomaterials in the biomedical domain. GelMA hydrogels emulate vital characteristics of the innate extracellular matrix (ECM) by integrating cell-adhering and matrix metalloproteinase-responsive peptide motifs. These features enable cellular proliferation and spreading within GelMA-based hydrogel scaffolds. Moreover, GelMA displays flexibility in processing, as it experiences crosslinking when exposed to light irradiation, supporting the development of hydrogels with adjustable mechanical characteristics. The drug delivery landscape has been reshaped by GelMA hydrogels, offering a favorable platform for the controlled and sustained release of therapeutic actives. The tunable physicochemical characteristics of GelMA enable precise modulation of the kinetics of drug release, ensuring optimal therapeutic effectiveness. In tissue engineering, GelMA hydrogels perform an essential role in the design of the scaffold, providing a biomimetic environment conducive to cell adhesion, proliferation, and differentiation. Incorporating GelMA in three-dimensional printing further improves its applicability in drug delivery and developing complicated tissue constructs with spatial precision. Wound healing applications showcase GelMA hydrogels as bioactive dressings, fostering a conducive microenvironment for tissue regeneration. The inherent biocompatibility and tunable mechanical characteristics of GelMA provide its efficiency in the closure of wounds and tissue repair.GelMA hydrogels stand at the forefront of biomedical innovation, offering a versatile platform for addressing diverse challenges in drug delivery, tissue engineering, and wound healing. This review provides a comprehensive overview, fostering an in-depth understanding of GelMA hydrogel’s potential impact on progressing biomedical sciences.

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“…Moreover, the layers displayed suitable injectability. Cytotoxicity assessments demonstrated the viability of cells exceeding 91% and 86% after 1 and 7 d, respectively [153].…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Pramanik,

Alhomrani,

Alamri

et al. 2024

Biomed. Mater.

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show abstract

“…The developed scaffold manufacturing techniques have helped researchers to fabricate multilayered scaffolds (compared to the single or bilayered ones), in which an intermediate layer exists between the bony and chondral layers 14,15 to resemble the role of native calcified cartilage. Such multilayered structures are anticipated to closely mimic the OC tissue microstructure and endure the joint shear forces after implantation 16 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of rabbit adipose derived stem cells fate in perfused multilayered silk fibroin composite scaffold for Osteochondral repair

Zadegan,

Vahidi,

Nourmohammadi

et al. 2024

J Biomed Mater Res

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Development of osteochondral tissue engineering approaches using scaffolds seeded with stem cells in association with mechanical stimulations has been recently considered as a promising technique for the repair of this tissue. In this study, an integrated and biomimetic trilayered silk fibroin (SF) scaffold containing SF nanofibers in each layer was fabricated. The osteogenesis and chondrogenesis of stem cells seeded on the fabricated scaffolds were investigated under a perfusion flow. 3‐Dimethylthiazol‐2,5‐diphenyltetrazolium bromide assay showed that the perfusion flow significantly enhanced cell viability and proliferation. Analysis of gene expression by stem cells revealed that perfusion flow had significantly upregulated the expression of osteogenic and chondrogenic genes in the bone and cartilage layers and downregulated the hypertrophic gene expression in the intermediate layer of the scaffold. In conclusion, applying flow perfusion on the prepared integrated trilayered SF‐based scaffold can support osteogenic and chondrogenic differentiation for repairing osteochondral defects.

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“…Nevertheless, these chemical cross-linking agents are toxic to cells. Another effective approach is to modify gelatin with a methacrylamide group i.e., GelMA 22 – 24 , which can form a thermo-irreversible hydrogel under ultraviolet irradiation. Furthermore, composite hydrogels can be obtained by blending GelMA with other polymers, which possess much higher viscosity than GelMA alone.…”

Section: Introductionmentioning

confidence: 99%

A dual-crosslinking electroactive hydrogel based on gelatin methacrylate and dibenzaldehyde-terminated telechelic polyethylene glycol for 3D bio-printing

Wang,

Yang,

Cai

et al. 2024

Sci Rep

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Gelatin was widely used as scaffold materials in 3D bio-printing due to its excellent bioactivity and availability and especially that their arginine–glycine–aspartic acid (RGD) sequences could efficiently promote cell adhesion and proliferation. In this study, an electroactive and 3D bio-printable hydrogel was prepared through a two-step chemical cross-linking process. Specifically, residual free amino groups of methacrylated gelatin (GelMA) were cross-linked with the aldehyde groups of dibenzaldehyde-terminated telechelic polyethylene glycol (DF-PEG) via Schiff base bonds, forming a gel at 37 °C. During the subsequent 3D bio-printing process, GelMA underwent UV curing, forming a secondary cross-linked network to the mechanical strength and stability of the printed structure. The uniform dispersion of carbon nanotubes (CNTs) in the GelMA/DF-PEG composite hydrogel significantly increased its conductivity. The optimized GelMA/DF-PEG composite hydrogel, i.e., 30% GelMA and 25% DF-PEG (G30D25-CNTs), exhibited superior bio-printability. When the content of CNTs was above 4%, the conductivity of G30D25-CNTs hydrogel exceeded 10–2 S/m, which satisfied the needs of cells for micro-current stimulation. Furthermore, the pore microstructures, swelling behavior, degradation ability and cell toxicity of G30D25-CNTs electroactive hydrogels were thoroughly evaluated. Thus, the G30D25-CNTs hydrogel with 4% MWCNTs could be considered for further application in electrical stimulation of tissue regeneration such as muscle and cardiac nerve tissue repair.

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A gradient four-layered gelatin methacrylate/agarose construct as an injectable scaffold for mimicking osteochondral tissue

Cited by 8 publications

References 98 publications

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Unveiling the versatility of gelatin methacryloyl hydrogels: a comprehensive journey into biomedical applications

Evaluation of rabbit adipose derived stem cells fate in perfused multilayered silk fibroin composite scaffold for Osteochondral repair

A dual-crosslinking electroactive hydrogel based on gelatin methacrylate and dibenzaldehyde-terminated telechelic polyethylene glycol for 3D bio-printing

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