A granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) produced by carrageenin‐induced inflammatory cells of mice

Shikita, Mikio; Tsuneoka, Kazuko; Hagiwara, Satoshi; Tsurufuji, Susumu

doi:10.1002/jcp.1041090118

Cited by 7 publications

(5 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore it is of key interest to investigate whether potential nanofilm coatings are able to suppress activation and the inflammatory response of macrophages upon adherence. Cytokines such as Interleukin 1 (IL-1), colony stimulating factor (CSF), and interferon 1 are known major chemical signals that recruit and activate other leukocytes near the site of implantation 40–43 . Interleukin 6 (IL-6) has also been identified as a useful indicator of inflammation in vivo 44–46 .…”

Section: Resultsmentioning

confidence: 99%

“…Cytokines such as interleukin 1 (IL-1), colony-stimulating factor (CSF), and interferon 1 are known major chemical signals that recruit and activate other leukocytes near the site of implantation. 40 -43 Interleukin 6 (IL-6) has also been identified as a useful indicator of inflammation in vivo. 44 -46 By monitoring these cytokines, we may be able to indicate whether our block copolymer films are capable of inhibiting aspects of the inflammatory response of macrophages in vitro.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Dynamic Cellular Adhesion Mediated by Copolymeric Nanofilm Substrates

Shin

Chen

Daram

et al. 2008

JALA: Journal of the Association for Laboratory Automation

View full text Add to dashboard Cite

Amphiphilic block copolymers are finding increased potential in biological and medical research due to their innate alternating hydrophilic and hydrophilic blocks/segments which can be used to package therapeutics, or coat a broad array of biological interfaces. Some studies are already directed towards utilizing these copolymers’ ability to form micelles or vesicles to develop novel methods of drug delivery to prevent inflammation or pro-cancer activity. Our study, however, aims to investigate the more fundamental cell-block copolymer interaction for use in protective nanofilms to prevent bio-fouling of non-tissue based implantable devices. Block copolymers could potentially fill the demand for biologically inert, highly functionalizable biomaterials desirable for this type of application. Two such polymers used in our study include PMOXA-PDMS-PMOXA triblock copolymer and PEO/PMMA diblock copolymer. Each block copolymer possesses hydrophilic and hydrophobic blocks that enable it to mimic the cell lipid membrane. So far we have shown that triblock copolymer is capable of inhibiting the accumulation of murine macrophages onto glass substrates. Preliminary evidence has suggested that the triblock copolymer has anti-adsorptive as well as non-inflammatory capabilities during short incubation periods (7 days) in vitro. While the diblock copolymer displays minimal anti-adsorptive activities, nanofilms comprised of a mixture of the two copolymers were able to significantly reduce macrophage accumulation onto glass substrates. The disparate behavior seen by macrophages on the different materials may be due to specific inherent properties such as preference for hydrophobic vs. hydrophilic surfaces and/or rough vs. smooth nano-textures. Furthermore, the specific end groups of the two polymers may exhibit varying capacities to resisting non-specific protein adsorption. Continued investigation outlining the physical and chemical properties desirable for an anti-adsorptive nano-film coating will serve as a basis upon which to design durable implant-tissue interfaces that can react to various external stimuli.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Dynamic Cellular Adhesion Mediated by Copolymeric Nanofilm Substrates

Shin

Chen

Daram

et al. 2008

JALA: Journal of the Association for Laboratory Automation

View full text Add to dashboard Cite

show abstract

“…The ability of these cells to respond with extreme rapidity by CSF release followed by increased CSF synthesis provides a very efficient system for ultra-rapid responses to such microorganisms. High local levels of CSF have been noted to occur within foci of inflammation (18).…”

Section: Cellular Sources Of the Csf'smentioning

confidence: 99%

The Role of the Colony‐Stimulating Factors in Resistance to Acute Infections

Metcalf

1987

Immunol Cell Biol

View full text Add to dashboard Cite

Summary.A set of specific glycoproteins, the colony-stimulating factors, has been identified as regulating granulocyte and macrophage production and function. These colony-stimulating factors have now been purified and mass produced by recombinant technology. These versatile regulators are capable of providing the body both with an ultrarapid and sustained system for responding to infections. The granulocytes, macrophages and eosinophils involved in these responses appear likely to be key cell populations ensuring adequate resistance to acute infections and the colony-stimulating factors may prove to be valuable agents in the clinic for increasing resistance to life-threatening infections particularly in immunologically compromised patients.The science of immunology arose out of attempts to understand and improve the manner in which the body responds to and overcomes infections. It was evident from the outset that resistance to infections involved the combined functional activity of a variety of white cells and a set of vaguely characterized reticuloendothelial cells. Granulocytes, macrophages and endothelial cells occupied an important position in early studies on resistance but, for the past forty years, immunology has been dominated by considerations of the mechanism of generation of specific antibodies and the functional activity of T and B lymphocytes. While in no way disputing the importance of antibodies and lymphocytes, particularly in suppressing second infections by the same organism, this preoccupation with lymphocytes led to an unfortunate down-modulation of work on the other cells involved in resistance. Watching the evolution of work on lymphocyte biology as an interested bystander, it seemed to me that the relatively slow proliferative and functional responses of lymphocytes were unlikely to provide the rapid response systems required for the initial control of infections by highly pathogenic organisms and that lymphocyte responses were relatively inefficient in handling certain types of infection, e.g. by fungi. In considering these shortcomings, it seemed more likely that effective early resistance relied heavily on the number, localization and functional activity of granulocytes and macrophages. However, in the absence of any sophisticated methods for influencing granulocyte-macrophage function, these were no more than idle convictions.Work in the past twenty years, and particularly in the last few years, has greatly increased our understanding of the molecular control of granulocyte-macrophage populations. This can best be exemplified by noting that clinical trials will be undertaken in the coming year using recombinant granulocytemacrophage colony-stimulating factors to increase the production and functional activity of granulocytes and macrophages in patients with depressed haemopoiesis and infections.It is useful therefore on this occasion to review briefly the present state of knowledge of the colony-stimulating factors and their actions in vivo in the context of the role these factors may...

show abstract

“…(а) GM-CSF is chemotactic for neutrophils (Gabrilove et al 1986) but also, possibly at higher concentrations, inhibits neutrophil locomotion (Gasson et al 1984). This combination of actions could result in an accumulation of neutrophils at a local inflammatory site, as such sites have been shown to contain high CSF concentrations (Shikita et al 1981).…”

Section: (D) Functional Stimulation Of Mature Cellsmentioning

confidence: 99%

The Wellcome Foundation Lecture, 1986 - The molecular control of normal and leukaemic granulocytes and macrophages

Metcalf

1987

Proc. R. Soc. Lond. B.

View full text Add to dashboard Cite

The development of semisolid culture methods supporting the clonal proliferation and maturation of granulocytes and macrophages led to the discovery of a group of specific glycoproteins, the colony - stimulating factors (CSFs), whose function it is to control the proliferation and functional activity of granulocytes, macrophages and associated blood cells. The four known CSFs in the mouse and man have been purified and complementary DNAs (cDNAs) for each have been cloned. The injection of bacterially synthesized recombinant CSF into mice has demonstrated that these CSFs can function in vivo to regulate granulocyte and macrophage formation. A major physiological role played by these CSFs is to control resistance to invading microorganisms through mechanisms capable of extremely rapid activation. Because the CSFs are the only known proliferative factors for these cells, the CSFs are involved in the initiation and the emergence of myeloid leukaemia but, conversely, at least one of the CSFs, G-CSF, is able to suppress myeloid leukaemic populations because of the ability of the CSFs to initiate differentiation commitment in responding granulocytic and macrophage populations. The CSFs are promising agents for clinical use in the treatment of infections in patients with depressed granulocyte-macrophage formation and possibly in the management of some types of myeloid leukaemia.

show abstract

A granulocyte‐macrophage colony‐stimulating factor (GM‐CSF) produced by carrageenin‐induced inflammatory cells of mice

Cited by 7 publications

References 23 publications

Dynamic Cellular Adhesion Mediated by Copolymeric Nanofilm Substrates

Dynamic Cellular Adhesion Mediated by Copolymeric Nanofilm Substrates

The Role of the Colony‐Stimulating Factors in Resistance to Acute Infections

The Wellcome Foundation Lecture, 1986 - The molecular control of normal and leukaemic granulocytes and macrophages

Contact Info

Product

Resources

About