A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells

Feng, Lingyan; Chen, Yong; Ren, Jinsong; Qu, Xiaogang

doi:10.1016/j.biomaterials.2011.01.002

Cited by 458 publications

(269 citation statements)

References 53 publications

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“…Owing to the semiselective nature of the interaction of AMPs with pathogenic bacteria, differentiation of multiple species of pathogenic bacteria has not been achieved. Future work will involve exploring strategies to improve this selectivity via investigations into multi-ligand 53,54 and aptamer-based capture agents 26,55 , and antibody-based biorecognition molecules with improved stability 56 to provide stringent discrimination between species of pathogenic bacteria. Alternative strategies for covalent and non-covalent functionalization of graphene sensors will also be explored 57 .…”

Section: Discussionmentioning

confidence: 99%

Graphene-based wireless bacteria detection on tooth enamel

et al. 2012

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Direct interfacing of nanosensors onto biomaterials could impact health quality monitoring and adaptive threat detection. Graphene is capable of highly sensitive analyte detection due to its nanoscale nature. Here we show that graphene can be printed onto water-soluble silk. This in turn permits intimate biotransfer of graphene nanosensors onto biomaterials, including tooth enamel. The result is a fully biointerfaced sensing platform, which can be tuned to detect target analytes. For example, via self-assembly of antimicrobial peptides onto graphene, we show bioselective detection of bacteria at single-cell levels. Incorporation of a resonant coil eliminates the need for onboard power and external connections. Combining these elements yields two-tiered interfacing of peptide-graphene nanosensors with biomaterials. In particular, we demonstrate integration onto a tooth for remote monitoring of respiration and bacteria detection in saliva. overall, this strategy of interfacing graphene nanosensors with biomaterials represents a versatile approach for ubiquitous detection of biochemical targets.

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Section: Discussionmentioning

confidence: 99%

Graphene-based wireless bacteria detection on tooth enamel

et al. 2012

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“…[12] The broad sensing potential of graphene can only be unlocked by the introduction of sensitizer (bio)molecules and structures, e.g. various inorganic groups, [23][24][25][81][82][83][84][85][86][87][88][89][90] organic or organometallic molecules, [37,[91][92][93][94][95][96] DNAs, [97][98][99][100][101] proteins, [102] peptides, [30,31,103,104] nanoparticles, [105,106,107] and 2D heterostructure. [51,52,61,108] These molecules are able to respond chemically or physically to their nearby environment, whose responses could then be transduced into an appreciable change in the conductivity of the carbon-based honeycomb scaffold.…”

Section: Meeting the Challenges In Chemical Functionalization Of Grapmentioning

confidence: 99%

“…Functionalizations of these defects with electrochemical catalysts lead to further improved sensitivity and selectivity for the detection of a wide range of molecules, namely glucose, [220] cholesterol, [221] DNA, [222,223] proteins, [224] and even living cells. [103,225] To functionalize graphene, most typical catalysts are composed of enzymes, [226] metal nanoparticles, [227] and polymers, [228] to name a few. In fact, to a large extent the functionalization of a GEC is similar to that of a GFET.…”

Section: Graphene-based Electrochemical (Gec) Biosensorsmentioning

confidence: 99%

Sensing at the Surface of Graphene Field‐Effect Transistors

et al. 2016

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Abstract. 10Recent research trends now offer new opportunities for developing the next generations of label-free biochemical sensors using graphene and other two-dimensional materials. While the physics of graphene transistors operated in electrolyte is well grounded, important chemical challenges still remain to be addressed, namely the impact of the chemical functionalizations of graphene on the key electrical parameters and the sensing performances. In fact, graphene -at least ideal graphene -is highly chemically inert. The 15 functionalizations and chemical alterations of the graphene surface -both covalently and non-covalently -are crucial steps that define the sensitivity of graphene. The presence, reactivity, adsorption of gas and ions, proteins, DNA, cells and tissues on graphene have been successfully monitored with graphene. This review aims to unify most of the work done so far on biochemical sensing at the surface of a (chemically functionalized) graphene field-effect transistor and the challenges that lie ahead. We are convinced that graphene biochemical sensors 20 hold great promises to meet the ever-increasing demand for sensitivity, especially looking at the recent progresses suggesting that the obstacle of Debye screening can be overcome.2

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“…The majority of structure-switching G4 electrochemical aptasensors were developed using gold electrodes, although, more recently, other electrochemical transducers have been employed, such as gold disk microelectrode arrays [70], modified platinum [71] or carbon electrodes [63,[71][72][73][74][75].…”

Section: Structure-switching G4 Electrochemical Aptasensormentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells

Cited by 458 publications

References 53 publications

Graphene-based wireless bacteria detection on tooth enamel

Graphene-based wireless bacteria detection on tooth enamel

Sensing at the Surface of Graphene Field‐Effect Transistors

Guanine Quadruplex Electrochemical Aptasensors

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