A graphene-modified cellulose paper microchip for HIV detection

Abstract

Rapid and inexpensive virus detection and quantification at the point-of-care is of paramount importance for HIV management in resource-limited settings. Here, we report on an easy-to-fabricate, cellulose paperbased microchip with printed graphene-modified electrodes for rapid detection of HIV-1 through electrical sensing. We evaluated the effect of electrode material and geometry on the performance of the microchip to detect serially diluted, electrically conductive samples. We evaluated the optimized microchip with HIVspiked samples.

Recent developments towards portable point-of-care diagnostic devices for pathogen detection

“…209 Viruses have also been detected using SPE-based electrical sensing techniques. For example, anti-gp-120 was immobilized on the surface of a paper microchip, and 10 8 copies mL −1 of HIV was detected with an impedance magnitude of 1 kHz frequency 210 (Fig. 4B).…”

Diagnosis of communicable diseases using papepr microfluidic platforms

“…Electrical sensing using printed graphene-modified electrodes on a cellulose paper has been done by Safavieh et al for the detection of HIV-1 [71]. A schematic of the microchip illustrating the virus capture process and its lysis is given in Figure 13.…”

Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring

“…Graphene-based sensors have also been decorated on flexible materials for HIV diagnostics [41]. In this work, a paper microchip modified with graphene-silver electrodes was designed to capture and detect HIV lysate on-chip.…”