A Graphene Oxide Based Immuno‐biosensor for Pathogen Detection

Abstract

Rotaviren auf der Spur: Die empfindliche und selektive Detektion von Rotaviren gelingt mithilfe der Photolumineszenz eines Graphenoxid(GO)‐Arrays. Die Zielzelle wurde vom Rotavirus‐spezifischen, auf dem GO‐Array immobilisierten Antikörper eingefangen, und der Bindevorgang wurde anhand der Fluoreszenzlöschung verfolgt, die aus dem resonanten Fluoreszenzenergietransfer (FRET) zwischen GO und Goldnanopartikeln an den Antikörpern resultiert (siehe Bild).

Surface Assembly of Graphene Oxide Nanosheets on SiO2 Particles for the Selective Isolation of Hemoglobin

“…This strategy could also be conveniently extended to the detection of other biomolecules, such as proteins [18] and adenosine triphosphate (ATP), [19] by substituting the dye-labeled DNA or MB with appropriate aptamers. The fluorescence resonance energy transfer between GO and the nanoparticles (NPs), for example, quantum dots (QDs) [20] and Au NPs, [21,22] has been used for the detection of DNA sequences and pathogens. In addition, polyethylene glycol (PEG)-functionalized GO has been recently used for cell imaging due to its intrinsic photoluminescence in the visible to the nearinfrared region.…”

Acetylcholinesterase‐Catalyzed Hydrolysis Allows Ultrasensitive Detection of Pathogens with the Naked Eye

“…Over the past decades, many approaches have been developed for pathogen detection, including the loop-mediated isothermal amplification (LAMP), [2] electrochemistry, [3] and nanotechnology, [4] just to name a few. However, most of the detection systems failed to monitor the pathogens in clinical samples where their concentrations are generally very low.…”

The pH Dependence of the Total Fluorescence of Graphite Oxide

“…It is manifest to assume that the bulk material actually suppresses this luminescence as quantum yields are quite low. Therefore, GO is rather used in quenching applications [7,37]. Figure 3 reveals that the pH dependent fluorescence of GO has two inflection points which are assigned to the carboxy acid and the aromatic hydroxy groups.…”